Cis-platinum drug-resistant lung cancer organoid, culture method thereof, medium used for culture of cis-platinum drug-resistant lung cancer organoid and application of cis-platinum drug-resistant lung cancer organoid

Abstract

The disclosure relates to a method for culture of a cis-platinum drug-resistant lung cancer organoid. The method comprises the following steps: mixing cis-platinum drug-resistant lung cancer tissue cells with the medium and matrix gel to obtain a to-be-cultured substance, wherein the medium contains matrix metalloproteinase-10, and the concentration of the matrix metalloproteinase-10 is 50-200nM based on the medium; and carrying out culture amplification on the to-be-cultured substance to obtain the cis-platinum drug-resistant lung cancer organoid. According to the culture method for the cis-platinum drug-resistant lung cancer organoid provided by the invention, since the employed medium contains the matrix metalloproteinase-10 with specific concentration, and the matrix metalloproteinase-10 with the specific concentration can promote in-vitro growth of the cis-platinum drug-resistant lung cancer organoid, the success rate of the culture is high when the method provided by the disclosure is used for the culture of the cis-platinum drug-resistant lung cancer organoid.

Description

technical field [0001] The disclosure relates to the technical field of biomedicine, and in particular to a method for cultivating cisplatin-resistant lung cancer organoids, a medium for culturing cisplatin-resistant lung cancer organoids, cisplatin-resistant lung cancer organoids and their use in drugs. filter in the application. Background technique [0002] Cisplatin, also known as cis-dichlorodiammine platinum, is a widely used anti-cancer chemotherapy drug in clinical practice. It has broad-spectrum anti-tumor properties. It has a good therapeutic effect in the treatment of tumor diseases such as gastric cancer and bladder cancer. However, in the process of using cisplatin for chemotherapy, cisplatin resistance will occur, which will easily lead to the failure of chemotherapy, which greatly limits the use of cisplatin in tumor chemotherapy. [0003] Lung cancer is a common malignant tumor in the world, and its death toll accounts for about 18.4% of all cancer deaths. ...

AI Technical Summary

Problems solved by technology

Drug concentration escalation and continuous action method usually takes 6 months or more to induce drug resistance, which takes a long time, and the mode of action of its chemotherapy drugs is significantly different from the principle of high-dose and short-course chemotherapy in clinical practice

The high-dose drug intermittent induction method induces drug resistance when the drug concentration is high, and the cells are often difficult to tolerate the sudden change of the culture environment, the induction success rate is low, and the cell state is difficult to control, and the drug resistance is unstable.

In addition, the drug-resistant cell lines induced by these two methods have obvious differences in cell morphology and biological characteristics, and belong to different cell sublines, resulting in the data obtained from drug screening in the two drug-resistant cell lines are often very different.