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Methods and materials for treating cancer

A technology for cancer and liver cancer, which is used in the field of cancer treatment and materials, and can solve problems such as ineffectiveness

Pending Publication Date: 2021-11-26
PENN STATE RES FOUND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Treatment for liver cancer often includes surgery and ablation, but this treatment is often ineffective in patients with advanced or end-stage disease

Method used

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  • Methods and materials for treating cancer
  • Methods and materials for treating cancer
  • Methods and materials for treating cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0092] Example 1: Transformation of human glioblastoma cells into neurons

[0093] GBM is the most prevalent and aggressive adult primary cancer of the central nervous system (CNS). The current standard GBM therapy is surgery followed by radiotherapy or chemotherapy, but due to the heterogeneity and highly aggressive nature of GBM, there has been little progress in treatment.

[0094] This example provides an alternative approach to treating GBM by reprogramming the transcription factors of malignant GBM cells (eg, Neurog2, NeuroD1 and / or Ascl1 reprogramming) into non-proliferative neurons.

[0095] cell culture

[0096] Human GBM cell lines were purchased from Sigma (U251) or ATCC (U118). U251 cells were cultured in GBM medium consisting of MEM (GIBCO), 0.2% penicillin / streptomycin (GIBCO), 10% FBS (GIBCO), 1 mM sodium pyruvate (GIBCO), 1% non-essential amino acids (NEAA, GIBCO) and 1x GlutMAX (GIBCO). U118 cells were cultured in a medium comprising DMEM (GIBCO), 10% FBS ...

Embodiment 2

[0125] Example 2: Transformation of liver cancer cells into non-cancerous liver cells

[0126] This example demonstrates that liver transcription factors (eg, GATA4, Foxa2, and / or HNF4A) can be used to mediate tumor cell reprogramming and transform tumor cells into normal-like cells, thereby establishing new strategies for treating liver cancer or other types of cancer.

[0127] cell line

[0128] Human hepatoma cell lines HepG2 and HEK293T (obtained from ATCC) were maintained in DMEM supplemented with 10% FBS and 1% penicillin / streptomycin. All cell lines are routinely used with MycoSolutions TM (AKRON) to detect mycoplasma contamination.

[0129] Antibody

[0130] Chicken polyclonal or mouse monoclonal antibodies specific for GFP were purchased from Abcam. Goat polyclonal antibodies against GATA4, Foxa2 and HNF4A proteins were obtained from R&D Systems. Mouse β-actin monoclonal antibody and goat albumin polyclonal antibody were from Santa Cruz, and rabbit GAPDH polyclon...

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Abstract

This document relates to methods and materials for treating a mammal having cancer. For example, methods and materials for converting one or more cancer cells present in a mammal with cancer into non-cancerous cells are provided.

Description

[0001] Cross References to Related Applications [0002] This application claims the benefit of U.S. Patent Application Serial No. 62 / 823,702, filed March 26, 2019. The disclosure of the prior application is considered part of the disclosure of the present application (and is incorporated herein by reference). technical field [0003] This document relates to methods and materials for treating mammals with cancer. For example, provided herein are methods and materials for converting one or more cancer cells present in a mammal with cancer to non-cancerous cells. Background technique [0004] Cancer is a major public health problem. In the United States alone, more than 1.7 million new cases were diagnosed in 2019 (National Cancer Institute, “Cancer Stat Facts: Cancer of Any Site”, “https:seer.cancer.gov / statfacts / html / all.html”. [0005] Glioblastoma (GBM), a tumor type arising from the unchecked proliferation of glial cells, accounts for half of all malignant brain tumor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K48/00A61P35/00C07K14/47A61K31/713C12N15/63C12N15/52
CPCA61K31/713A61P35/00C07K14/47A61K48/005C12N2740/16043
Inventor 陈功王欣裴子飞
Owner PENN STATE RES FOUND