Application of a composition and test kit thereof in detecting glycocholic acid
A kit and a technique for glycocholic acid, which are applied in the field of medical detection, can solve problems such as cumbersome processes, and achieve the effects of avoiding cumbersome processes, simple preparation procedures and high sensitivity.
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preparation example Construction
[0096] In some embodiments, a preparation method of glycocholic acid detection kit is provided, comprising:
[0097] 1) Preparation of glycocholic acid-biotin
[0098] The preparation principle of glycocholic acid-biotin is as follows:
[0099]
[0100] Raw materials include glycocholic acid; NH 2 -PEG-Biotin is an amino PEG biotin derivative, the molecular weight of PEG can be 300-3000; condensing agents include but are not limited to N,N'-dicyclohexylcarbodiimide, 1-ethyl-(3-dimethyl Aminopropyl)carbodiimide hydrochloride, N,N'-diisopropylcarbodiimide, O-(7-azabenzotriazol-1-yl)-N,N,N', At least one of N'-tetramethylurea hexafluorophosphate and benzotriazole-N,N,N',N'-tetramethylurea hexafluorophosphate; catalysts include but are not limited to 4-difluorophosphate At least one of methylaminopyridine, 1-hydroxybenzotriazole, and 4-pyrrolidinopyridine; solvents include but are not limited to dichloromethane, tetrahydrofuran, ether, ethyl acetate, dimethyl sulfoxide, N,N ...
Embodiment 1
[0113] In this example, the reagents used for pH adjustment were 2.0M hydrochloric acid and 2.0M sodium hydroxide aqueous solution.
[0114] In the present embodiment, the source of each raw material and product information are as follows:
[0115] Manufacturer of polystyrene carboxyl microspheres: JSR (Beijing Bormaier agent); product name: latex microspheres; product number: P0113.
[0116] Manufacturer of Glycocholic Acid: Aladdin; Product Name: Glycocholic Acid; Product Number: G131002.
[0117] Antibody: Manufacturer: Tansheng Technology; Product Name: CG Rabbit Polyclonal Antibody; Product Number: FAB-F005.
[0118] NH 2 -PEG-Biotin: Manufacturer: Shanghai Tuoyang; Product Name: Biotin Polyethylene Glycol Amino; Product Number: P002009.
[0119] 1. Preparation of glycocholic acid reagent
[0120] 1.1 Preparation of glycolic acid-biotin derivatives
[0121] To a dry round bottom flask was added glycocholic acid (46.5 mg, 0.10 mmol), NH 2-PEG-Biotin (PEG molecular we...
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