A liquid injection device for pcr system with self-replacing needles

Abstract

The invention relates to a PCR system liquid injection device for self-replacing needles, comprising a support plate assembly, a pipetting box assembly, a pipetting needle assembly and a pipetting pump assembly, the pipetting box assembly is installed on the support plate assembly, the The pipette box assembly is used to hold different types of pipette needles, and the pipette box assembly moves horizontally on the support plate assembly; the pipette needle assembly is located above the pipette box assembly, and the pipette needle assembly moves vertically Moving up and down in the vertical direction, the pipetting needle assembly is used for pipetting and replacing the pipetting needle; the pipetting pump assembly is located behind the pipetting needle assembly, and is used to communicate with the pipetting needle and drive the pipetting needle to perform suction and discharge operations , the device uses the joint movement of the pipetting needle holder and the pipetting needle push plate to realize the withdrawal and replacement of the pipetting needle in batches, and the pipetting needle push plate operates smoothly during the needle withdrawal process. The liquid process is batched and automated, which greatly improves the pipetting speed and improves the pipetting efficiency.

Description

technical field [0001] The invention belongs to the technical field of in vitro diagnostic analysis instruments, and relates to a liquid injection device for a PCR system with self-replacing needles. Background technique [0002] Polymerase chain reaction (PCR) is a molecular biology technique used to amplify specific DNA fragments. It can be regarded as a special DNA replication outside the body. The biggest feature of PCR is that it can greatly increase the amount of trace DNA. , in order to apply PCR technology to a large extent, the instruments and equipment specially used for PCR experiments have been applied and developed accordingly. PCR equipment can be well controlled between denaturation temperature, annealing temperature and extension temperature, so that specific DNA fragments can be copied in large quantities increase, repeated pipetting is essential during the course of the experiment. [0003] The traditional pipetting method is to use a single-channel or mul...

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Problems solved by technology

[0004] Chinese patent document CN109142709A discloses a puncture pipetting device of a time-resolved fluorescence immunoassay analyzer. On the back of the frame, the output shaft of the piercing pipetting stepping motor faces upwards, and is connected to the screw drive in the piercing pipetting screw drive assembly through the piercing liquid pipetting synchronous belt assembly; the nut in the piercing pipetting screw drive assembly is installed on the large sliding plate On the back, the puncture pipetting stepping motor drives the screw to rotate, and the nut matched with the screw drives the large sliding plate to slide up and down, together with the pipetting ejection assembly and the needle ejection selection assembly to move up and down; due to the pipetting ejection on the large sliding plate The assembly is interspersed with a needle ejection head selection assembly, which realizes both puncture and liquid transfer in the same mechanism. However, the needle ejection process in this device is cumbersome, and the needle ejection and replacement processes of pipetting needles cannot be performed in batches. This patent still cannot solve the problem. The pipetting process is fast and the batch automatic pipetting process, the pipetting efficiency is low

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