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Anti-varicella-zoster virus siRNAs and application thereof

A technology of herpes zoster virus and chickenpox, which is applied in the field of biomedicine, can solve the problems of limited population for neurovirulence, retention of vaccines, and no effective therapeutic drugs, and achieves inhibition of virus replication, inhibition of replication, and inhibition of cell-to-cell transmission. Effect

Active Publication Date: 2021-12-07
NORTHWESTERN POLYTECHNICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are still major challenges in the prevention and treatment of VZV. There is no specific treatment drug, and the existing vaccines also have problems such as the risk of neurovirulence and the limitation of the applicable population.

Method used

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  • Anti-varicella-zoster virus siRNAs and application thereof
  • Anti-varicella-zoster virus siRNAs and application thereof
  • Anti-varicella-zoster virus siRNAs and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] Example 1: Regulatory effect of si-ORF7 and si-ORF68 on target genes (ORF7, ORF68)

[0068] 1. qPCR detection of the knockdown effect of the target gene

[0069] (1) siRNAs transfection and virus infection

[0070] Human retinal pigment epithelial cells (ARPE19) were divided into 1×10 5 Inoculate into a 12-well plate, and after the cells adhere to the wall, replace the medium with blank DMEM / F12, add si-ORF7 and si-ORF68 to a certain amount of blank DMEM / F12 medium, and add transfection reagent lipo2000 Incubate in a certain amount of blank DMEM / F12 medium for 5 minutes, then add lipo2000 and DMEM / F12 incubations to si-ORF7, si-ORF68 and DMEM / F12 incubations, mix well and let stand for 20 minutes, then incubate The substances were added to the corresponding 12-well plate, the final concentration of si-ORF7 and si-ORF68 was 10 nM, and the medium was changed to 2% FBS DMEM / F12 medium 6 hours after transfection, and VZV was infected with a certain multiplicity of infecti...

Embodiment 2

[0086] Embodiment 2: si-ORF7, si-ORF68 antiviral effect evaluation

[0087] 1. WB detection of siRNAs antiviral effect

[0088] (1) siRNAs transfection and virus infection

[0089] Human retinal pigment epithelial cells (ARPE19) were divided into 2×10 5 Inoculate into a 6-well plate, and after the cells adhere to the wall, replace the medium with blank DMEM / F12, add si-ORF7 and si-ORF68 to a certain amount of blank DMEM / F12 medium, and add transfection reagent lipo2000 to A certain amount of blank DMEM / F12 medium was incubated for 5 minutes, and then the lipo2000 and DMEM / F12 incubations were added to the si-ORF7, si-ORF68 and DMEM / F12 incubations, mixed and allowed to stand for 20 minutes, and then the incubations were added Added to the corresponding 6-well plate, the final concentration of si-ORF7 and si-ORF68 was 10nM, and the medium was changed to 2% FBS DMEM / F12 medium 6h after transfection, and VZV virus was infected with a certain multiplicity of infection (MOI=0.3) ...

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Abstract

The invention discloses anti-varicella-zoster virus siRNAs. The anti-varicella-zoster virus siRNAs comprise a positive-sense strand and an antisense strand which are complementary, wherein the nucleotide sequence of the positive-sense strand is one of sequences shown in SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27 and 29, or is a sequence which has the similarity of 90 percent or above with one of the nucleotide sequences of the positive-sense strand; and the nucleotide sequence of the antisense strand of the siRNAs is one of 15 sequences corresponding to the positive-sense strand respectively, or is a complementary sequence of a sequence which has the similarity of 90 percent or above with one of the nucleotide sequences of the positive-sense strand. Moreover, the invention further discloses application of the anti-varicella-zoster virus siRNAs. The siRNAs disclosed by the invention have very high biological activity, can remarkably inhibit replication of varicella-zoster virus, and have the advantages of high functionality and the like.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to siRNAs against varicella-zoster virus and application thereof. Background technique [0002] Varicella Zoster Virus (VZV) is a ubiquitous and highly contagious human α-subfamily-herpesvirus. Initial infection with VZV can lead to chickenpox, and the population is generally susceptible (infection rate is about 61% to 100%). The virus can be latently infected in the dorsal root ganglia for life. Chickenpox causes a huge disease burden worldwide, with at least 140 million new cases of chickenpox, 4.2 million cases of severe complications of chickenpox, and 4200 deaths every year. In addition, nearly one-third of VZV-infected people will develop herpes zoster due to reactivation of latent nerve VZV in old age, usually accompanied by severe neuralgia, and even after healing, postherpetic neuralgia will occur, severe affect the patient's quality of life. Chickenpox ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113A61K31/713A61P31/22
CPCC12N15/1133A61P31/22C12N2310/14
Inventor 骞爱荣裴佳伟田野李郁陈志浩杨超飞
Owner NORTHWESTERN POLYTECHNICAL UNIV
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