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Method for detecting influence of different fire intensities on underground soil seed bank

A technology of soil seed bank and strength, applied in the field of fire impact on underground soil seed bank, can solve the problems of insufficient impact research, and achieve the effect of deepening the understanding of vegetation spatial pattern and time evolution mechanism

Active Publication Date: 2021-12-21
NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, domestic and foreign research on grassland fire mainly focuses on the effects of fire on soil temperature, soil microorganisms and biomass, plant diversity and productivity, and soil respiration, while the impact of fire intensity on underground soil seed banks is insufficient.

Method used

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  • Method for detecting influence of different fire intensities on underground soil seed bank
  • Method for detecting influence of different fire intensities on underground soil seed bank
  • Method for detecting influence of different fire intensities on underground soil seed bank

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Experimental program
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specific Embodiment approach 1

[0022] Specific implementation mode one: the method for detecting the impact of different fire intensities on the underground soil seed bank in this implementation mode:

[0023] 1. Sampling of soil seed bank by soil core method;

[0024] 2. Cutting steel pipe;

[0025] 3. Steel pipe bottom sealing and embedding: put nylon mesh on the bottom of the steel pipe cut in step 2, and then put it into the groove. The distance between the steel pipes in the groove is more than 10cm, and then use soil to connect the groove with the steel pipe, The gap between the steel pipe and the steel pipe is filled;

[0026] 4. Filling the culture substrate: pass the culture substrate through a 1cm sieve, wash it with water, and then place it in an oven at 100°C for 48 hours, then put the dried culture substrate into the steel pipe in the groove of step 3 and fill it evenly. The upper surface of the culture medium is 5-10cm away from the top of the steel pipe;

[0027] 5. Lay the temperature pro...

specific Embodiment approach 2

[0037] Embodiment 2: The difference between this embodiment and Embodiment 1 is that in Step 1, 10 ± 5 soil cores are randomly selected in each quadrat, and the depth of the soil cores is 5 ± 0.5 cm. Other steps and parameters are the same as those in the first embodiment.

[0038] The soil cores of this embodiment can collect soil seed banks with different soil depths, such as soil cores with a soil depth of 0 to 5 cm, soil cores with a soil depth of 5 to 10 cm, soil cores with a soil depth of 10 to 15 cm, and soil cores with a soil depth of 10 to 15 cm. Soil cores at 15 to 20 cm levels. The soil cores collected from the same square and at the same depth were mixed and the impurities and large-grained stones in the soil were removed as test samples.

[0039] In this embodiment, the responses of soil seed banks at different depths to fire situations can be detected, and the responses of soils at different depths to the same fire situations can be vertically compared.

[0040...

specific Embodiment approach 3

[0041] Specific embodiment 3: The difference between this embodiment and specific embodiment 1 or 2 is that it is characterized in that the diameter of the soil core sampling soil drill in step 1 is 5 cm. Other steps and parameters are the same as those in Embodiment 1 or 2.

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Abstract

According to the method for detecting the influence of different fire intensities on the underground soil seed bank, the fire intensities are accurately controlled through simple operation, and basic data are provided for detecting the influence of the fire intensities on the soil seed bank. The detection method comprises the following steps: 1, sampling a soil seed bank by adopting a soil core method; 2, cutting the steel pipe; 3, sealing the bottom of the steel pipe and embedding; 4, filling a culture medium; 5, laying a temperature probe on the upper surface of the culture medium in the steel pipe, and then laying the soil seed bank sample in the step 1 on the culture medium and the temperature probe; 6, embedding an isolation frame around the steel pipe; 7, burning treatment; 8, after the hay is burnt, covering the isolation frame with a nylon net; and 9, performing conventional watering management, performing regular weeding, recording the number of seedlings and species types, measuring plant height and biomass, and further comparing influences of different fire intensities on species composition and growth conditions of the soil seed bank.

Description

technical field [0001] The invention relates to a method for the influence of fire on underground soil seed bank. Background technique [0002] The soil seed bank refers to the litter in the upper layer of the soil and all living seeds in the soil. It has the dual functions of remembering the history of vegetation and affecting the recovery ability of vegetation. It is the source material provider for vegetation renewal and reproduction. The soil seed bank can maintain the balance of population dynamics under short-term environmental changes or disturbances, and determine the direction of vegetation succession. It can effectively restore and rebuild vegetation, promote the health of regional ecosystems, and increase biodiversity. The Fifth International Conference on Seed Ecology in 2016 took the environmental control factors of soil seed bank dynamics as a special topic. The 6th International Conference on Seed Ecology in 2019 will focus on the mechanisms and processes of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N31/12
CPCG01N31/12
Inventor 赵丹丹马红媛李绍阳亓雯雯夏江宝武海涛
Owner NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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