Probe, primer group, kit and detection method for rapidly detecting aeromonas salmonicida through RPA-LFS

A technology of Aeromonas salmonicida and primer set, applied in the direction of microorganism-based methods, biochemical equipment and methods, DNA/RNA fragments, etc., can solve the problems of difficult identification of non-specific amplification results and complex primer design, etc. Achieve rapid detection, good specificity, and simple operation

Pending Publication Date: 2021-12-24
JIANGSU OCEAN UNIV
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Problems solved by technology

However, loop-mediated isothermal amplification technology also has many shortcomings, such as complex primer design, once non-specific amplification occurs, it is difficult to identify

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  • Probe, primer group, kit and detection method for rapidly detecting aeromonas salmonicida through RPA-LFS
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  • Probe, primer group, kit and detection method for rapidly detecting aeromonas salmonicida through RPA-LFS

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Embodiment Construction

[0025] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. The following description of at least one exemplary embodiment is merely illustrative in nature and in no way taken as limiting the invention, its application or uses. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0026] It should be noted that the terminology used here is only for describing specific implementations, and is not intended to limit the exemplary implementations according to the present application. As used herein, unless the context clearly dictates otherwise, the singular is intended to in...

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Abstract

The invention relates to the field of biological detection, in particular to a probe, a primer group, a kit and a detection method for rapidly detecting the aeromonas salmonicida through RPA-LFS. According to the probe and the primer group for rapidly detecting the aeromonas salmonicida through the RPA-LFS, the sequence of the probe is shown as SEQ ID NO.1, and the sequences of the primer group are respectively SEQ ID NO.2 and SEQ ID NO.3. The invention further discloses the detection method for detecting the aeromonas salmonicida, the reaction is rapid and sensitive, and the operation is simple.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to a probe, a primer set, a kit and a detection method for rapid detection of Aeromonas salmonicida by RPA-LFS. Background technique [0002] The detection of Aeromonas salmonicida in the prior art mainly includes traditional culture methods, immunodiagnosis and nucleic acid detection. The culture method mainly relies on conventional microbial physiological and biochemical identification methods. The operation process is cumbersome and time-consuming, which often delays the diagnosis of the disease. For immunodiagnosis, Tong Shangliang et al. used ELISA technology to establish a method for the rapid detection of Aeromonas salmonicida in water samples. The principle of the method is to combine the antigen-antibody immune reaction and the efficient catalytic reaction of the enzyme to achieve visual observation of the results. , but the technical operation process is cumbersome, lo...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/689C12Q1/6844C12N15/11C12R1/01
CPCC12Q1/689C12Q1/6844C12Q2521/507C12Q2565/625
Inventor 司鑫鑫谷庆花高嵩李铭远张晓赵陈洁
Owner JIANGSU OCEAN UNIV
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