Method for rapidly screening high-temperature-resistant desert algae species

A desert algae and high temperature-resistant technology, applied in the field of desert algae screening, can solve problems such as heat resistance of cyanobacteria that have not been screened in large quantities

Active Publication Date: 2021-12-28
新疆金正生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are relatively few studies on the heat resistance of desert algae at home and abroad, and these studies are carried out on the heat resistance of one or two algae species, and there is no large-scale screening of cyanobacteria for heat resistance research.

Method used

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  • Method for rapidly screening high-temperature-resistant desert algae species
  • Method for rapidly screening high-temperature-resistant desert algae species
  • Method for rapidly screening high-temperature-resistant desert algae species

Examples

Experimental program
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Effect test

Embodiment 1

[0022] One inventive method for rapid screening of algae desert temperature, comprising the steps of:

[0023] Step a, soil samples were collected Desert: sand collecting shovel algae-free skin layer and the like to 10cm deep, collected in a sterile sealed bag, after each sampling wiper blade Anabaena with 70% alcohol, to prevent cross-contamination; Select desert soil samples, the samples treated with a 20 mesh screen to remove debris in the sample;

[0024] Step two, the activation of the desert soil samples algal cells: culture medium prepared, sterilized cool in the laboratory 500mL said activation vessel desert soil sample taken was placed a sterilized culture medium containing 300mL of thoroughly mixed, placed in light culture shelves, the light intensity of 5000 Lux, light dark cycle 14h / 10h, cultured under conditions of a temperature of 28 ℃ 2 weeks per day early in the evening ventilation for one hour to give the algae culture solution;

[0025] Step three, separation, ...

Embodiment 2

[0030] One inventive method for rapid screening of algae desert temperature, comprising the steps of:

[0031] Step a, soil samples were collected Desert: sand collecting shovel algae-free skin layer and the like to 10cm deep, collected in a sterile sealed bag, after each sampling wiper blade Anabaena with 70% alcohol, to prevent cross-contamination; Select desert soil samples, the samples treated with a 20 mesh screen to remove debris in the sample;

[0032] Step two, the activation of the desert soil samples algal cells: culture medium prepared, sterilized cool in the laboratory 500mL said activation vessel desert soil sample taken was placed a sterilized culture medium containing 300mL of thoroughly mixed, placed in light culture shelves, the light intensity of 5000 Lux, light dark cycle 14h / 10h, cultured under conditions of a temperature of 29 ℃ 2 weeks per day early in the evening ventilation for one hour to give the algae culture solution;

[0033] Step three, separation, ...

Embodiment 3

[0038] One inventive method for rapid screening of algae desert temperature, comprising the steps of:

[0039]Step 1. Collect the desert soil sample: collect the sand sample layer with noga showers and the following to 10cm deep, collected from the sterile sealing bag, and use 70% alcohol and nogae for each sampling, to prevent cross-pollution; choose the desert Soil samples, the samples were treated with 20 mesh sieves, and the samples were removed;

[0040] Step 2, activate the algae cells in the desert soil sample: formulate the culture solution, sterilize and cool, and weigh the desert soil samples in the laboratory to be placed in a 500ml activation container containing 300 ml of culture medium, fully mixed. On the lighting culture shelf, in the light intensity of 5000lux, the optical dark period was 14 h / 10 h, the temperature was 30 ° C for 2 weeks, and each morning and evening were ventilated, and the algae liquid was obtained.

[0041] Step three, separation, purification ...

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Abstract

The invention relates to a method for rapidly screening high-temperature-resistant desert algae species. In a mass culture process of algae species, the temperature is a main limiting factor influencing the growth and reproduction of the algae species, the heat-resistant algae capable of surviving and growing under the high-temperature condition can be rapidly screened in a large scale, the excellent algae species can be provided for algae desertification control research, high-temperature water bath treatment is adopted, treatment at different temperatures is set, therefore, the growth conditions of algae cells at different temperatures are obtained, and an experimental basis is provided for selecting proper environmental conditions and time periods during algae species field inoculation and spraying in algae biological desertification control.

Description

Technical field [0001] The present invention relates to the field of desert algae screening techniques, in particular to a method for the rapid screening of algae desert temperature. Background technique [0002] Taklimakan Desert in Xinjiang is a typical inland arid desert region, not only is the largest desert and is the world's second largest mobile desert. Survival algae desert environment over a long period of adaptation of heat, cold, drought, high light, strong ultraviolet radiation habitats, evolved a unique physical, biological activity, and contains new genes corresponding thereto. [0003] At present, domestic and foreign research on algae desert heat resistance is still relatively small, and these studies were conducted in a heat resistance or two species of algae, cyanobacteria did a lot of heat resistance screening study. Studies have shown that the algae in the desert sand to reduce fixed sandstorm species play an important role. Therefore, the important ecological...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12N1/02C12R1/89
CPCC12N1/12C12N1/02
Inventor 梁钧
Owner 新疆金正生物科技有限公司
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