Arthrobacter sp. and application thereof in degradation of aristolochic acid
A technology of aristolochic acid and Arthrobacter, applied in the field of microorganisms, can solve the problems of unremoved nitro groups, etc., and achieve the effects of fast reproduction rate, complete detoxification and fast growth rate
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0028] Example 1 Screening Arthrobacter sp. NyZ610
[0029] (1) Prepare culture medium
[0030] Tryptone soybean broth liquid medium: Tryptone soybean broth powder 6g, distilled water 1000 mL, sterilized at 121°C for 21 minutes.
[0031] Tryptone soybean broth solid medium: tryptone soybean broth powder 6g, agar 15g, distilled water 1000mL, sterilized at 121°C for 21 minutes.
[0032] Basic inorganic salt liquid medium: 14.3g disodium hydrogen phosphate dodecahydrate, 3g potassium dihydrogen phosphate, 0.28mg manganese sulfate, 0.3mg ferrous sulfate, 0.06mg magnesium sulfate, 1mg calcium chloride, 0.05mg copper sulfate, sulfuric acid Zinc 0.05mg, boric acid 0.05mg, distilled water 1000ml, pH 7.0.
[0033] Basic inorganic salt solid medium: 14.3 g disodium hydrogen phosphate dodecahydrate, 3 g potassium dihydrogen phosphate, 0.28 mg manganese sulfate, 0.3 mg ferrous sulfate, 0.06 mg magnesium sulfate, 1 mg calcium chloride, 0.05 mg copper sulfate, sulfuric acid Zinc 0.05mg, ...
Embodiment 2
[0042] Example 2 Degradation of Aristolochic Acid I by Arthrobacter sp. NyZ610
[0043] (1) The aristolochic acid-degrading bacteria NyZ610 screened in Example 1 was inoculated on a tryptone soybean broth solid plate, and cultured at 30° C. for 10 h. Use an inoculation loop to scrape a small amount of bacterial cells from the above plate into 5mL tryptone soybean broth liquid medium, cultivate at 30°C, 180r / min for 8h to OD 600 About 0.8. The above bacterial liquid was centrifuged at 5000 g for 5 min at 4°C to collect the bacterial cells. Wash the bacteria 3 times with sterilized basic inorganic salt liquid medium, and set aside.
[0044] (2) Resuspend the bacteria collected above in 100 μL basic inorganic salt culture solution, inoculate into 5 mL basic inorganic salt culture solution containing 200 μM aristolochic acid I, and initially inoculate OD 600 About 0.004, cultured at 30°C, 1800r / min, regularly sampled to measure the concentration and OD of aristolochic acid 600...
Embodiment 3
[0046] Example 3 Degradation of Aristolochic Acid II by Arthrobacter sp. NyZ610
[0047] (1) The aristolochic acid-degrading bacteria NyZ610 screened in Example 1 was inoculated on a tryptone soybean broth solid plate, and cultured at 30° C. for 10 h. Use an inoculation loop to scrape a small amount of bacterial cells from the above plate into 5mL tryptone soybean broth liquid medium, cultivate at 30°C, 180r / min for 8h to OD 600 About 0.8. The above bacterial liquid was centrifuged at 5000 g for 5 min at 4°C to collect the bacterial cells. Wash the bacteria 3 times with sterilized basic inorganic salt liquid medium, and set aside.
[0048] (2) Resuspend the bacteria collected above in 100 μL basic inorganic salt culture solution, inoculate into 5 mL basic inorganic salt culture solution containing 200 μM aristolochic acid II, and initially inoculate OD 600 About 0.004, cultured at 30°C, 1800r / min, regularly sampled to measure the concentration and OD of aristolochic acid 6...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More