59 results about "Aristolochic acid I" patented technology

The invention discloses a method of screening a marker of acute renal toxicity caused by aristolochic acid by utilizing cell metabolic profiling in vitro. The effective dose causing renal cell damage is determined by adopting a 3-(4,5-dimethylthiahiazo-2)-2,5-diphenytetrazoliumromide (MTT) staining manner. Renal cell metabolites are analyzed by adopting an ultra-high performance liquid chromatography-mass chromatography technique to obtain metabolic profiles. Metabolic profile data of normal groups and medicine damage groups are analyzed by adopting a multivariate statistic method. A potential renal toxicity marker is screened by combining a change rule of metabolites along with medicine acting time. The screened marker is good in predicting performance. The average accuracy of outer verification is 90% or above. The method comprehensively represents variation situations of metabolites of normal renal cells under medicine damages, and can provide technique supports for early diagnosis of renal toxicity caused by medicines or medicine safety evaluation.

The invention relates to a method for detecting active ingredients of Longdan Xiegan particles and belongs to the field of modernization of Chinese traditional medicine. According to the invention, the method for detecting the Longdan Xiegan particles is standardized; on the basis of original identification on gardenia, by establishment of identification and content determination on gentian, identification and content determination on baical skullcap root and liquorice and content determination on gardenia serving as ministerial drug, ingredients of the medicament and compatibility of the ingredients can be more comprehensively detected and content and stability of the Longdan Xiegan particles can be more comprehensively known; the method is also helpful to explaining the medicinal material basis of the medicament; and meanwhile, due to increase of examination on aristolochic acid, the method is more beneficial for reducing toxic and side effects of the Longdan Xiegan particles.

A method for analyzing compositions of aristolochine and aristoloactam in blood and tissue includes utilizing high efficiency of liquid phase chromatography to analyze two said compositions in blood and in tissue simultaneously as using chromatographic column of bonded silica gel, flowing phase of 1% glacial acetic aid / 30mmol.L-1 triethylamine-acetonitrile gradient elution, column temperature of 15deg.c, detection wavelength of 250nm or 260nm.

The invention provides a method for simultaneously detecting salicylic acid, aristolochic acid A, sodium cyclamate and beta-naphthol. According to the method, in the extraction process, the interference of partial interference substances in a sample to be detected to the salicylic acid, the aristolochic acid A, the sodium cyclamate and the beta-naphthol is shielded; moreover, when mass spectrometric detection is carried out, the contents of the salicylic acid, the aristolochic acid A, the sodium cyclamate and the beta-naphthol in the sample to be detected are more accurately tested in an electro-spraying ionization anion and multi-reaction monitoring scanning mode through quantitative determination of second-level ion fragments. Besides, by utilizing the method provided by the invention, the detection on the salicylic acid, the aristolochic acid A, the sodium cyclamate and the beta-naphthol is simultaneously realized, the interference of similar compounds is eliminated, the accuracy and the reliability of the detected components are ensured, the detection sensitivity is high, the repeatability is good, and high accuracy and precision are achieved; the method is applicable to rapid quantitative screening and evaluation on the salicylic acid, the aristolochic acid A, the sodium cyclamate and the beta-naphthol in cigarette additives.

The invention discloses a quality detection method for a Tibetan medicine composition rheumatism Theron preparation. With the adoption of the quality detection method, the quality standard of an existing rheumatism Theron preparation is correspondingly improved and an identification method for shellac and elecampane in a previous standard is optimized; the identification on juniperus formosana, syzygium aromaticum, meconopsis, rubia wallichiana decne and amomum kravanh is increased on the basis of the previous standard, and a content determination method of general flavone and swertia chirayita and a detection method for aristolochic acid A are increased, so as to further ensure the safe, efficient, uniform and stable product quality and the controllable quality.

A method for detecting the content of four disabled components (aristolochic acid, salicylic acid, nordihydroguaiaretic acid and cinnamyl anthranilate) in flavors and fragrances comprises the steps of preparing a sample solution, performing liquid chromatogram analysis, calculating detected results and the like. After optimized, the method has the advantages of being short in detection time, simple and convenient to operate, high in sensitivity and recovery rate, good in repeatability and the like. The chromatographic condition of the method enables the aristolochic acid, the salicylic acid, the nordihydroguaiaretic acid and the cinnamyl anthranilate to be separated well, the method has good correlation, a detecting range is limited from 0.04mug to 0.34mug, the average recovery rates are respectively 94.01%, 95.96%, 86.48% and 84.28%, and average relative standard deviations of sample testing results are respectively 4.40%, 2.39%, 2.49% and 3.35%.

The invention discloses a method for constructing a mouse liver cancer model by using aristolochic acid I or combination of the aristolochic acid I and carbon tetrachloride. The method comprises the following steps of performing intraperitoneal injection on a mouse to perform administration of the aristolochic acid I or the combination of the aristolochic acid I and the carbon tetrachloride; and selecting the treated mouse to carry out dissection, tumor statistics and pathohistochemical judgment on the liver cancer of the mouse. The method provides a powerful tool for researching a liver cancer induction mechanism of the aristolochic acid I, mutation and mutation fingerprints caused by the aristolochic acid I in a cancer process and treatment of the liver cancer induced by the aristolochicacid I.

The present invention relates to aristolochic acid and processing method for its blood plasma or blood serum of lactam, which is characterized in that exacts to the sample aforementioned by acetic acid ethyl ester after acidification by hydrochloric acid. Adds hydrochloric acid to sample of blood plasma and serum (make sure the concentration is 3.0í‡10^-5~ 1.0í‡10^-4molíñmL^-1)and eddy mix; lay for 30~60min at room temperature. Use acetic acid ethyl ester to exact for 1~2 times with 3~5ml per time, collect supernatant after eddy mixing. Use water-bath and nitrogen for exacted supernatant under 60 Deg to dry; add 150uL carbinol for leavings to dissolve; filter, take 20uL filtrate to check by HPLC. The blood plasma or blood serum aforementioned in the invention comprises human, rat, mouse, rabbit, etc. Rate of extraction and recovery for aristolochic acid and its lactam in sample of blood plasma or blood serum is more than 95%.

The invention discloses an extract enriched in benzopyran lactone as well as a preparation method and medical application of the extract. The extract provided by the invention is enriched in gastrodinA, gastrodin B and gastrodin C, wherein the sum of contents of the three is not lower than 90%; or the extract is enriched in gastrodin A, gastrodin B, gastrodin C and gastrodin D, and the sum of contents of the four is not lower than 85%. The extract is clear in monomer composition, high in content and capable of easily controlling the quality, the renal toxicity of aristolochic acid can be reduced, and the extract can be used for preparing a single Chinese herb containing aristolochic acid or a traditional Chinese medicine tract or an attenuated drug of a traditional Chinese medicine compound preparation. According to the preparation method of the extract, disclosed by the invention, separation methods that are difficult to operate on a large scale in a factory, such as column chromatography on silica gel, do not need to be repeatedly used, the extract with the total content 85% or higher of effective monomer composition can be prepared by only passing through a primary macroporousresin and combining with pH regulation and control, and the preparation method is capable of easily realizing industrialization, simple in operation and low in cost.

The invention relates to a detection method of a radix gentianae liver-fire clearing granule, which belongs to the field of modernization of traditional Chinese medicine. The detection method of the radix gentianae liver-fire clearing granule, which is disclosed by the invention, can more comprehensively detect the components of drugs and the compatibility thereof and know the content and stability of the components by establishing the identification of monarch drug radix gentianae, the identification of minister drug radix scutellariae and guide drug liquorice and the content measurement of the minister drug radix scutellariae on the basis of the original identification of fructus gardeniae, is conductive to describing the pharmaceutic material basis of the drugs, increases the inspection on aristolochic acid and is more conductive to reducing the toxic side effect.

The invention provides a rapid aristolochic acid A detection card and a detection method of aristolochic acid A, and belongs to the technical field of aristolochic acid A detection. The rapid aristolochic acid A detection card adopts the structure that a test strip is arranged in a casing of the rapid aristolochic acid A detection card; a sample pad, a colloidal gold (or latex particle) membrane, a cellulose nitrate membrane and a water-absorbing membrane are sequentially pasted on a supporting backplane; the colloidal gold (or latex particle) membrane is a glass fiber membrane containing an anti-aristolochic acid A monoclonal antibody colloidal gold (or latex particle) marker; two detection tapes are transversely arranged on the cellulose nitrate membrane, one detection tape contains an aristolochic acid protein conjugate, and the other detection tape is a quality control tape containing an anti-rabbit antibody or an anti-mouse antibody. The detection method utilizes an immunological method to directly detect the residual quantity of aristolochic acid A in traditional Chinese medicinal materials or Chinese patent drugs. The rapid aristolochic acid A detection card is easy to prepare, convenient and quick to use, and accurate in detection result.

The invention belongs to the field of biomedicine and particularly relates to a method for measuring the content of an aristolochic acid compound in traditional Chinese medicine. Contents of aristolochic acid A, aristolochic acid B, aristolochic acid C and aristolochic acid D in aristolochic acid traditional Chinese medicine can be measured at the same time, quality evaluation of the traditional Chinese medicine containing the aristolochic acid compound can be completed, and scientific bases can also be provided for safety evaluation of the aristolochic acid compound.

The invention discloses an aristolochic acid fluorescent test paper and a preparation method and application thereof, and belongs to the technical field of the medicine analysis. The preparation method comprises the following steps: firstly synthesizing 1,4-di(4-oxybutyrate) benzene, and then iodizing the benzene ring by using KIO3 and I2, polymerizing with the 1,4-diethylbenzene under the catalysis of the tetrakis(triphenylphosphine)palladium to obtain polymer precursor PPE-OBA; synthesizing the oxybutyric acid as oxybutyrate polyethylene glycol through esterification reaction on the PPE-OBAside chain, and obtaining an aristolochic acid fluorescent probe PPE-PEG, wherein the structure is as shown in description; finally soaking the filter paper strip in a PPE-PEG solution for 30sec, placing in a dark room for 1 hour after taking out from the PPE-PEG solution, thereby obtaining the aristolochic acid fluorescent test paper. The fluorescent test paper disclosed by the invention is convenient for preparation, low in cost, obvious in effect and has the potential for large-scale manufacturing; the aristolochic acid can be quickly detected on the spot in real time by naked eyes by onlyneeding a handheld ultraviolet lamp, and the test paper has extensive application prospect.

An aristolochic acid A monoclonal antibody hybridoma cell strain and an application thereof. The invention belongs to the field of food safety immunodetection. There is provided an aristolochic acid Amonoclonal antibody hybridoma cell strain SS0709, which is preserved in China General Microbiological Culture Collection Center and September 5th, 2017 and is assigned the accession number of CGMCC No.14688. An aristolochic acid A monoclonal antibody is secreted by the aristolochic acid A monoclonal antibody hybridoma cell strain SS0709 of which the accession number is CGMCC No.14688. The application of the aristolochic acid A monoclonal antibody is to analyze and detect the aristolochic acid A in traditional Chinese medicines. The monoclonal antibody secreted by the cell strain has excellentspecificity and detection sensitivity (IC50 being 4 ng/mL) to the aristolochic acid A, so that the monoclonal antibody can achieve the detection on the aristolochic acid A in the traditional Chinesemedicines. The cell strain supplies a raw material to the immunodetection on the aristolochic acid A in the traditional Chinese medicines and has practical application value.

The invention belongs to the biotechnical field and the traditional Chinese medicinal detection field, and discloses a preparation method of an anti-aristololactam FI (AL-FI) and aristolochic acid IVa (AA-IVa) monoclonal antibody, and an application of the monoclonal antibody The anti-AL-FI monoclonal antibody is prepared by synthesizing the artificial immune antigen and the coating antigen of the AL-FI through the structural reconstruction of the AL-FI, and a competitive enzyme linked immunosorbent assay method is established through the monoclonal antibody. The anti-AL-FI and AA-Iva monoclonal antibody is a first monoclonal antibody aiming at AL compounds and has a high antibody sensitivity, and the affinity constant (Ka) of the monoclonal antibody to the AL-FI is 1.941*10<9>M<-1>. The monoclonal antibody can simultaneously identify the AA-IVa, and the cross reaction rate of the monoclonal antibody is 42.27%. A process for the qualitative/quantitative analysis of the AL-FI and/or the AA-IVa having renal cytotoxicity in relevant medicinal materials and biological samples through utilizing the monoclonal antibody based enzyme linked immunosorbent assay method has the advantages of simple, sensitive and rapid sample pretreatment, environmental protection and the like.

The invention provides application of aristolochic acid in preparation of immunosuppressive drugs, especially application in drugs treating rheumatoid arthritis. The aristolochic acid gel provided by the invention can effectively inhibit rheumatoid arthritis and has no renal toxic reaction, thus providing a new choice for clinical treatment of rheumatoid arthritis.

The invention discloses aristolochic acid derivatives and an application thereof. The specific structure of the aristolochic acid derivatives is shown as a formula (I). The aristolochic acid derivative provided by the invention is separated from the root of an asarum plant and is identified as a new compound through a structure, and experiments prove that the compound has good activity in the field of sterilization, such as common staphylococcus aureus, especially bacterial diseases in agricultural pathogenic bacteria,meanwhile, experiments prove that the compound also has a good application prospect in meat preservation.

The invention discloses a preparation method of a fluorescent sensor for detecting aristolochic acid A. The method comprises the following steps that carbon quantum dots (CQDs) are synthesized, and ovalbumin-stabilized gold nano-clusters (OVA@AuNCs) are synthesized, and after the carbon quantum dots and the ovalbumin-stabilized gold nano-clusters are dissolved by ultra-pure water, the carbon quantum dots and the ovalbumin-stabilized gold nano-clusters are mixed uniformly to obtain the fluorescent sensor for detecting the aristolochic acid A; during detection, the carbon quantum dots are takenas a main body, and the fluorescence of the carbon quantum dots is enhanced by using OVA @ AuNCs, and the fluorescence in the CQDs- OVA@AuNCs is quenched through the addition of the aristolochic acidA; and the CQDs-OVA@AuNCs serves as a fluorescence sensing platform, and the aristolochic acid A is quantitatively detected in a fluorescence quenching mode. According to the method, the defects thatan existing technical method for detecting the aristolochic acid A is too complex, the detection speed is low, and the recognition performance of the aristolochic acid A is low are overcome. The method is simple, convenient, fast, high in controllability, high in efficiency, suitable for industrial production and wide in market application prospect.

The invention discloses a preparation method of a temperature-sensitive magnetic molecularly imprinted polymer for selectively separating and enriching aristolochic acid I. The temperature-sensitive magnetic molecularly imprinted polymer is prepared by the following steps: (1) preparing graphene oxide; (2) preparing magnetic graphene oxide; (3) preparing silicon dioxide coated magnetic graphene oxide; (4) taking the silicon dioxide coated magnetic graphene oxide as a carrier, taking aristolochic acid I as a template molecule, taking N-isopropylacrylamide as a temperature-sensitive functional monomer, taking methacrylic acid as a functional monomer, taking divinyl benzene as a cross-linking agent and taking azodiisobutyronitrile as an initiator, and preparing the temperature-sensitive magnetic molecularly imprinted polymer with specific recognition capability on aristolochic acid I on the surface of the carrier. The temperature-sensitive magnetic molecularly imprinted polymer prepared by the method can be rapidly separated under an external magnetic field, has relatively high specific adsorption capacity and recognition rate on a target compound, can be repeatedly used, is reversible and controllable in adsorption/desorption process along with temperature change, and is environment-friendly.

The invention provides a method for building a mouse renal anemia animal model. The method comprises the following steps: 1) preparing a 0.4 mg/mL aristolochic acid solution from aristolochic acid with PBS (Phosphate Buffer Saline); 2) injecting the aristolochic acid solution into the enterocoelia of a mouse for 6 weeks, 3 mg/kg once every 3 days, and detecting anemia, renal function index, renalpathology and capability of secretion of hemopoietin; 3) stopping administration for 6 weeks, and observing the previous indexes again. The mouse renal anemia animal model built by the method is stable and has high modeling rate, and the problem that renal anemia has a higher incidence rate among chronic kidney diseases but a mature renal anemia animal model is lacked at present is solved.

The invention discloses a method for performing limit test on aristolochic acid A in a Zhuifengtougu capsule and belongs to a high performance liquid chromatography method. According to the method, 0.5 percent glacial acetic acid and methanol serve as flowing phase and are subjected to gradient elution. By adoption of the method, impurity peak interference at the peak position of an aristolochic acid A control product can be eliminated, the specificity is more excellent than that of the existing method, the durability is high, quality control can be realized well and the medicine safety is benefited.

The invention discloses a preparation method and application of a magnetic temperature-sensitive molecularly imprinted polymer based on a deep eutectic solvent system, and belongs to the technical field of functional material preparation. The N-isopropylacrylamide/(3-acrylamidopropyl) trimethyl ammonium chloride eutectic solvent system is used as a functional monomer, so that the adsorption capacity of an imprinted product is improved, and the reusability is relatively high. The molecularly imprinted polymer is used as a dispersive solid-phase extraction adsorbent, and is combined with a high performance liquid chromatography to establish a novel high-selectivity separation and enrichment method for rhein in a semen cassiae sample. In order to further prove the applicability of the molecular imprinting technology, another two magnetic temperature-sensitive molecularly imprinted polymers are prepared by adopting the eutectic solvent system as a functional monomer, and can be used for efficient analysis of triterpenoid saponin compounds and aristolochic acid substances; the application value of the magnetic temperature-sensitive molecularly imprinted polymer based on the eutectic solvent system in complex sample analysis is disclosed.

The invention discloses a nucleic acid aptamer capable of specifically recognizing aristolochic acid A and application of the nucleic acid aptamer for the first time and belongs to the field of bioengineering. According to the nucleic acid aptamer and the application thereof, the aristolochic acid A is adopted as a target, a cpature-SELEX technology is adopted to screen aptamers having the specific recognition ability on the aristolochic acid A, the aptamer having the highest occurrence frequency in a high-throughput sequencing result is selected, and the performance of the nucleic acid aptamer is further improved through truncation and construction of a stable secondary structure. The nucleic acid aptamer has the characteristics of being easy to synthesize and modify, good in stability and the like, and can be applied to construction of a rapid detection method for aristolochiaceae traditional Chinese medicines, traditional Chinese medicines possibly containing the aristolochic acid A and products of the traditional Chinese medicines.

The invention discloses a method for detecting aristolochic acid I content in Zhuifengtougu capsules based on a UPLC method and a sample pretreatment method. According to the method, a Zhuifengtougu capsule sample is pretreated in a manner of combining liquid-liquid extraction and solid-phase extraction, wherein liquid-liquid extraction is acid regulation extraction, so that not only can an emulsification phenomenon be effectively prevented from occurring in a sample preparation process, but also a solid-phase extraction small column can be effectively prevented from being blocked by sample loading liquid, meanwhile, the recovery rate of aristolochic acid I is guaranteed, and a very good impurity removal effect is achieved. According to the invention, the content of aristolochic acid I inthe Zhuifengtougu capsules is further analyzed in combination with UPLC, specific pretreatment and chromatographic detection conditions are adopted, the aristolochic acid I can be effectively separated in the liquid chromatography, the separation degree reaches 1.5 or above, the impurity interference is extremely small, the content of the aristolochic acid I in the Zhuifengtougu capsules can be effectively, simply, conveniently and accurately detected, and then the safety and stability of clinical medication of the Zhuifengtougu capsules are ensured.

The invention discloses a method for determining the content of six aristolochic acid components in asarum, and belongs to the technical field of traditional Chinese medicine, a UPLC-MS/MS method is adopted for simultaneously determining the content of aristolochic acid A, aristolochic acid B, aristolochic acid C, aristolochic acid D, aristolochic acid III and aristolochic acid VIIa in asarum, the specificity is higher, more components are controlled, different medicinal parts are comprehensively measured and analyzed, so that the quality of the medicinal material is more comprehensively evaluated, and a basis is provided for reasonable application of the asarum medicinal material.

The invention relates to an HPLC-FLD-DAD analysis method for detecting a biomarker of aristolochic acid nephropathy (AAN), namely, aristolochic acid-DNA (AA-DNA) adducts. In the method, according to fluorescent characters of the AA-DNA adducts, HPLC-FLD is used for detecting the AA-DNA adducts while HPLC-DAD is used for detecting deoxynucleosides, thereby measuring the content of the adducts. The method can simultaneously assay two most main adducts of AAN: a deoxyadenosine-aristolochic acid I adduct (dA-AA-I) and a deoxyadenosine-aristolochic acid II adduct (dA-AA-II), and mainly includes the steps of DNA extraction, DNA digestion, enrichment of the adducts, HPLC-FLD for assaying the AA-DNA adducts, HPLC-DAD for detecting the deoxynucleosides, and calculation of the content of the AA-DNA adducts. The method is used for detecting the content of the AA-DNA adducts in animal tissue (e.g. liver or kidney tissue of a mouse) that is dosed with drugs or foods that contain the AAs, such as caulis aristolochiae manshuriensis.