Preparation method of fluorescent sensor for detecting aristolochic acid A

A fluorescent sensor and aristolochic acid technology, applied in the field of fluorescent sensors, can solve the problems of low identification of aristolochic acid A, complex detection method of aristolochic acid A, slow detection speed, etc., and achieve high sensitivity and controllable High performance and good stability

Active Publication Date: 2019-04-16
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The invention aims to solve the problems that the existing aristolochic acid A detection method is too complex, the detection speed is slow

Method used

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  • Preparation method of fluorescent sensor for detecting aristolochic acid A
  • Preparation method of fluorescent sensor for detecting aristolochic acid A
  • Preparation method of fluorescent sensor for detecting aristolochic acid A

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1: The preparation method of the fluorescence sensor for detecting aristolochic acid A is as follows:

[0032] (1) Add 250 mg of activated carbon powder, 22.5 mL of concentrated sulfuric acid, and 7.5 mL of concentrated nitric acid to a round bottom flask and reflux for 5 hours at 80°C. After cooling to room temperature, add 2 times the volume of the mixed acid to the reaction product plus ultrapure water , Adjust the pH to 13 with 6mol / L sodium hydroxide and put it in a hydrothermal reactor. After reacting at 160℃ for 14 hours, adjust the pH to 7.0 with 1mol / L sulfuric acid, and dialyze the neutral product with 1000 molecular weight The bag was dialyzed for 2 days, and the product in the dialysis bag was freeze-dried to obtain carbon quantum dots (CQDs). Transmission electron micrographs of carbon quantum dots ( figure 1 , 2 );

[0033] (2) Put 5mL, 10mg / mL ovalbumin (OVA) liquid and 1mL, 10mmol / L chloroauric acid solution into a beaker, sonicate for 5min and stir a...

Embodiment 2

[0035] Embodiment 2: The preparation method of the fluorescence sensor for detecting aristolochic acid A is as follows:

[0036] (1) Add 250 mg of activated carbon powder, 19.4 mL of concentrated sulfuric acid, and 5.6 mL of concentrated nitric acid to a round bottom flask at 90°C and reflux for 6 hours. After cooling to room temperature, add 3 times the volume of the mixed acid to the reaction product plus ultrapure water. Adjust the pH of the solution to 12 with 5mol / L sodium hydroxide and put it into a hydrothermal reactor. After reacting at 170°C for 13 hours, adjust the pH to 7.0 with 1mol / L sulfuric acid. Dialysis the neutral product with a 1000 molecular weight dialysis bag In 3 days, freeze-dry the product in the dialysis bag to obtain carbon quantum dot powders (CQDs);

[0037] (2) Put 20mL, 8mg / mL ovalbumin (OVA) solution and 5mL, 8mmol / L chloroauric acid solution into a beaker, sonicate for 10min and stir at room temperature for 25min, adjust the pH of the solution to 12...

Embodiment 3

[0039] Example 3: Experiment of OVA@AuNCs to enhance the fluorescence of CQDs

[0040] Gradually add 10μL 0.01mg / mL OVA@AuNCs solution to 2mL 0.2mg / mL CQDs solution, adding 10μL each time, it can be seen that the fluorescence intensity of CQDs solution reaches the highest value when it is added to 30μL OVA@AuNCs solution, and then gradually decreases ( Picture 9 ).

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Abstract

The invention discloses a preparation method of a fluorescent sensor for detecting aristolochic acid A. The method comprises the following steps that carbon quantum dots (CQDs) are synthesized, and ovalbumin-stabilized gold nano-clusters (OVA@AuNCs) are synthesized, and after the carbon quantum dots and the ovalbumin-stabilized gold nano-clusters are dissolved by ultra-pure water, the carbon quantum dots and the ovalbumin-stabilized gold nano-clusters are mixed uniformly to obtain the fluorescent sensor for detecting the aristolochic acid A; during detection, the carbon quantum dots are takenas a main body, and the fluorescence of the carbon quantum dots is enhanced by using OVA @ AuNCs, and the fluorescence in the CQDs- OVA@AuNCs is quenched through the addition of the aristolochic acidA; and the CQDs-OVA@AuNCs serves as a fluorescence sensing platform, and the aristolochic acid A is quantitatively detected in a fluorescence quenching mode. According to the method, the defects thatan existing technical method for detecting the aristolochic acid A is too complex, the detection speed is low, and the recognition performance of the aristolochic acid A is low are overcome. The method is simple, convenient, fast, high in controllability, high in efficiency, suitable for industrial production and wide in market application prospect.

Description

Technical field [0001] The invention belongs to the field of fluorescence sensors, and specifically relates to a preparation method of a fluorescence sensor for detecting aristolochic acid A. Background technique [0002] Nephrotoxic aristolochic acid is a nitrophenanthroline carboxylic acid derivative, which is derived from plants of the Aristolochiaceae family, such as Aristolochia and Asarum. Most of the ingredients have been confirmed to be nephrotoxic, carcinogenic and Mutagenesis. Among them, aristolochic acid A (AAs) is the most toxic and has attracted widespread attention. These AAs-containing herbs have been widely used to treat tumors, snakebites, small pox, rheumatism and pneumonia, until AAs are proven to be carcinogens and mutagens that seriously harm human kidney toxins. Although, the use of herbs containing AAs has been banned worldwide. However, these herbs are still sold through the Internet, and the abuse of AAs-containing herbs has been repeatedly banned. T...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/6428G01N2021/6432
Inventor 李灿鹏谭双赵卉
Owner YUNNAN UNIV
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