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Rapid aristolochic acid A detection card and detection method of aristolochic acid A

A technique of aristolochic acid and detection card, which is applied in the directions of measuring devices, instruments, scientific instruments, etc., can solve the problems of complicated operation, etc., and achieve the effects of high sensitivity, easy preparation, and saving detection costs.

Inactive Publication Date: 2015-09-09
长沙安迪生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The object of the present invention is to provide a kind of detection apparatus that can detect aristolochic acid A quickly, directly apply the method of immunology to detect the content of aristolochic acid A in Chinese medicinal material or Chinese patent medicine, overcome the complicated operation of existing chromatographic detection and other shortcomings, establish a detection method based on immunological specificity

Method used

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  • Rapid aristolochic acid A detection card and detection method of aristolochic acid A

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preparation example Construction

[0037] 1. Example of Preparation of Anti-Aristolochic Acid A Antibody

[0038] 1. Preparation of aristolochic acid A immune antigen and coating antigen

[0039] Weigh 80 mg of aristolochic acid A, add 15 mg of glutaric anhydride, dissolve in 4 ml of pyridine, and react at room temperature for 24 hours. Then dry it with nitrogen, add 8ml dimethyl sulfoxide-dioxane (1:1), add 60μL tributylamine, stir in a water bath for 10 minutes, then add 30μL isobutyl chloroformate, and stir for 1 hour. Add 100 mg of bovine serum albumin (BSA) dissolved in pH 8.5 borate buffer or 70 mg of chicken ovalbumin (OVA) dissolved in pH 8.5 borate buffer dropwise in a cold bath, and react at room temperature After 12 hours, the reaction product was dialyzed against 0.01mol / L pH7.5 phosphate buffered saline (PBS) for 3 days, freeze-dried, and stored at -20°C.

[0040] 2. Preparation of monoclonal antibody against aristolochic acid A;

[0041] 2.1 Animal immunity;

[0042] Dissolve the synthetic immun...

Embodiment 1

[0054] A quick test card for aristolochic acid A, such as figure 1 As shown, it is provided with test strip 2 in the test card shell 1, and the test card shell 1 is a long flat thin shell-shaped shell with a length of 70mm, a width of 20mm, a thickness of 5mm, a thin shell wall thickness of 1mm, and is made of engineering plastics. The detection card housing 1 is composed of two halves connected with a housing cover and a housing seat, and a detection window 11 and a sample injection hole 12 are arranged on the housing cover of the detection card housing 1 . The test strip 2 is placed in the shell seat of the test card shell 1 .

[0055] like figure 2 As shown, the test strip 2 is a narrow sheet with a multilayer structure, the length of the sheet is about 60mm, the width of the sheet is about 4mm, and the thickness is about 2.5mm. The test strip 2 has a multi-layer structure, the bottom layer is a supporting backboard 21, which is a polyvinyl chloride plastic sheet, about ...

Embodiment 2

[0057] The difference between this embodiment and the detection card embodiment 1 is: (1) the colloidal gold film 23 is a latex particle film, and the latex particle film is a glass fiber film containing an anti-aristochlic acid A antibody latex particle label; (2) Contained in the quality control band 242 is an anti-rabbit antibody.

[0058] Three, detection method embodiment

[0059] The detection method of this embodiment is to adopt the detection card described in the detection card embodiment 1 to detect the content of aristolochic acid A in Chinese medicinal materials such as Chuanketong, Guangfangji, Guanmutong or Chinese patent medicine samples containing aristolochic acid medicinal materials. Content, the specific processing method is as follows:

[0060] ①Extraction sample solution: take Chinese herbal medicines such as Chuanketong, Guangfangji, Tianxianteng, Qingmuxiang, Guanketong and Aristolochia into fine powder, pass through a 60-mesh sieve (0.30mm aperture), a...

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Abstract

The invention provides a rapid aristolochic acid A detection card and a detection method of aristolochic acid A, and belongs to the technical field of aristolochic acid A detection. The rapid aristolochic acid A detection card adopts the structure that a test strip is arranged in a casing of the rapid aristolochic acid A detection card; a sample pad, a colloidal gold (or latex particle) membrane, a cellulose nitrate membrane and a water-absorbing membrane are sequentially pasted on a supporting backplane; the colloidal gold (or latex particle) membrane is a glass fiber membrane containing an anti-aristolochic acid A monoclonal antibody colloidal gold (or latex particle) marker; two detection tapes are transversely arranged on the cellulose nitrate membrane, one detection tape contains an aristolochic acid protein conjugate, and the other detection tape is a quality control tape containing an anti-rabbit antibody or an anti-mouse antibody. The detection method utilizes an immunological method to directly detect the residual quantity of aristolochic acid A in traditional Chinese medicinal materials or Chinese patent drugs. The rapid aristolochic acid A detection card is easy to prepare, convenient and quick to use, and accurate in detection result.

Description

technical field [0001] The invention relates to a quick detection card for aristolochic acid A and a detection method thereof, is a detection device for detecting aristolochic acid A, and belongs to the technical field of detection of aristolochic acid A. Background technique [0002] Aristolochic acid compounds are a kind of nitrophenanthrene compounds unique to Aristolochiaceae plants, including aristolochic acids A, B, C and D, etc. The Aristolochiaceae Aristolochia plants that are used as medicine in my country include Aristolochia, Guan Mutong, Guangfangji, Zhushalian, Aristolochia dayelia, Hanzhong Fangji, Xugufeng, etc. These traditional Chinese medicines contain aristolochic acid and Its nitrophenanthrene organic acid derivatives or aristolochic lactam components. It was found that such compounds can inhibit tumor cells in the early stage, and later found that they have analgesic, anti-inflammatory, expectorant, diuretic, blood pressure regulation and other effects a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N33/577
CPCG01N33/558G01N33/577
Inventor 周坚汤朝阳李云峰
Owner 长沙安迪生物科技有限公司
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