Method for mensurating in vivo sample of containing aristolochic acid and components of lactam categories

Abstract

A method for analyzing compositions of aristolochine and aristoloactam in blood and tissue includes utilizing high efficiency of liquid phase chromatography to analyze two said compositions in blood and in tissue simultaneously as using chromatographic column of bonded silica gel, flowing phase of 1% glacial acetic aid / 30mmol.L-1 triethylamine-acetonitrile gradient elution, column temperature of 15deg.c, detection wavelength of 250nm or 260nm.

Description

technical field [0001] The invention relates to a high performance liquid chromatography method for simultaneously determining aristolochic acid and its lactam components in blood (plasma, serum, red blood cells) and tissues (heart, liver, spleen, lung, kidney, brain, stomach, bladder). Background technique [0002] Aristolochic acid I and II (aristolochic acid I, AA-I; aristolochic acid II, AA-II) are the main active ingredients contained in plants of the genus Aristolochia, and they are also toxic ingredients. The body of AA-I and AA-II The main metabolites are aristolactam I and II (aristololactam I, AL-I; aristololactam II, AL-II). Due to the report of "Aristolochic Acid Nephropathy" (Aristolochic Acid Nephropathy, AAN), the detection and research of the content of such components in the body has become an important task. [0003] There are some previous reports on the analysis and detection methods of the distribution of aristolochic acid compounds in the body. The me...

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Problems solved by technology

The method for detecting AA-I and II has paper chromatography-spectrophotometry (K.Hideg, Olga H.Hankovszky, J.Méhes.Estimation of aristolochic acid and some of its derivatives paper chromatography and spectrophotometry in body fluids, Acta Physiologica, 1963 , 23:79-84), UV spectrophotometry (Xiang Zhengxin, He Xingquan, Zhou Guifen, etc. UV spectrophotometry and pharmacokinetic study of aristolochic acid content. Acta Pharmaceutica Sinica, 1984, 19(3) : 224~227), gas chromatography (Lai Puhui, Jiang Guiji, Pei Wen. Preparation of aristolochic acid-A and synthesis of its derivatives. Chemical Bulletin, 1990, (1): 33~35) and isotope labeling method (Su Tao, Qu Lei, Zhang Chunli, et al. Study on the metabolic characteristics of aristolochic acid I in rats. Chinese Journal of Traditional Chinese Medicine. 2004, 29 (7): 676-681), etc.; aristolochic lactam compounds Detection, with high performance liquid chromatography-fluorescence method for the determination of AL-I, AL-II, and AL-I in urine and feces a (G.Krumbiegel, et al.Studies on the metabolism of aristolochic acids I and II, Xenobiotica, 1987,17(8):981-991), and the determination of AL-I and AL-II in human urine (Schulz M, Weist F, Gemahlich M. Dunnschichtchromatographischer Nachweis der Aristolochia-Saure inverschiedenen Korperflussigkeiten. Arznein Forsch, 1971, 21: 934-936), but there is no report on the simultaneous detection of AAs and ALs in blood and tissues

At present, there is only one invention patent of our research group ( Application number: 200410033845.7 ) provided a high-performance liquid chromatography method for the simultaneous determination of aristolochic acid I, II and aristolochic lactam I, II in plasma, but there was no report on the determination of such components in red blood cells, and no aristolochic acid in tissue There are only reports on the determination of lactam components, but only reports on the determination of aristolochic acids in tissues

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