Method for mensurating in vivo sample of containing aristolochic acid and components of lactam categories

A technique for aristolochic lactam and aristolochic acid, which is applied in the field of high performance liquid chromatography and can solve problems such as unseen and unseen aristolochic lactams and the like

Inactive Publication Date: 2007-05-09
PEKING UNIV
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Problems solved by technology

The method for detecting AA-I and II has paper chromatography-spectrophotometry (K.Hideg, Olga H.Hankovszky, J.Méhes.Estimation of aristolochic acid and some of its derivatives paper chromatography and spectrophotometry in body fluids, Acta Physiologica, 1963 , 23:79-84), UV spectrophotometry (Xiang Zhengxin, He Xingquan, Zhou Guifen, etc. UV spectrophotometry and pharmacokinetic study of aristolochic acid content. Acta Pharmaceutica Sinica, 1984, 19(3) : 224~227), gas chromatography (Lai Puhui, Jiang Guiji, Pei Wen. Preparation of aristolochic acid-A and synthesis of its derivatives. Chemical Bulletin, 1990, (1): 33~35) and isotope labeling method (Su Tao, Qu Lei, Zhang Chunli, et al. Study on the metabolic characteristics of aristolochic acid I in rats. Chinese Journal of Traditional Chinese Medicine. 2004, 29 (7): 676-681), etc.; aristolochic lactam compounds Detection, with high performance liquid chromatography-fluorescence method for the determination of AL-I,...

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  • Method for mensurating in vivo sample of containing aristolochic acid and components of lactam categories
  • Method for mensurating in vivo sample of containing aristolochic acid and components of lactam categories
  • Method for mensurating in vivo sample of containing aristolochic acid and components of lactam categories

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Embodiment Construction

[0079] 1. Determination of aristolochic acid and aristolochic lactam components in rat plasma

[0080] In order to lay the foundation for the determination of aristolochic acid and its lactam components in the plasma or serum of patients, we used Guan Mutong water decoction (concentrations were 6g·ml -1 , 4g·ml -1 ) gavage SD male rats, simulating clinical aristolochic acid nephropathy patients, after obtaining the drug-containing plasma, adopt the method of the present invention to carry out pretreatment and HPLC detection to the drug-containing plasma, and carry out the method of the present invention and the protein precipitation method commonly used Compare.

[0081] Implementing the concrete steps of the present invention comprises:

[0082] (1) Chromatographic conditions

[0083]Chromatographic column: ZORBAX SB-C18 (4.6mm×250mm, 5μm), Agilent company

[0084] Mobile phase: A:B=1% acetic acid / 30mmol·L -1 Triethylamine: Acetonitrile (35%B→100%B)

[0085] Column temp...

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Abstract

A method for analyzing compositions of aristolochine and aristoloactam in blood and tissue includes utilizing high efficiency of liquid phase chromatography to analyze two said compositions in blood and in tissue simultaneously as using chromatographic column of bonded silica gel, flowing phase of 1% glacial acetic aid / 30mmol.L-1 triethylamine-acetonitrile gradient elution, column temperature of 15deg.c, detection wavelength of 250nm or 260nm.

Description

technical field [0001] The invention relates to a high performance liquid chromatography method for simultaneously determining aristolochic acid and its lactam components in blood (plasma, serum, red blood cells) and tissues (heart, liver, spleen, lung, kidney, brain, stomach, bladder). Background technique [0002] Aristolochic acid I and II (aristolochic acid I, AA-I; aristolochic acid II, AA-II) are the main active ingredients contained in plants of the genus Aristolochia, and they are also toxic ingredients. The body of AA-I and AA-II The main metabolites are aristolactam I and II (aristololactam I, AL-I; aristololactam II, AL-II). Due to the report of "Aristolochic Acid Nephropathy" (Aristolochic Acid Nephropathy, AAN), the detection and research of the content of such components in the body has become an important task. [0003] There are some previous reports on the analysis and detection methods of the distribution of aristolochic acid compounds in the body. The me...

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Application Information

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IPC IPC(8): G01N30/06G01N1/28G01N30/02
Inventor 王璇许俊羽李晓玫高小丽蔡小青
Owner PEKING UNIV
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