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Method for measuring content of aristolochic acid compound in traditional Chinese medicine

A technique for the determination of aristolochic acid and its determination method, which is applied in the field of biomedicine and can solve the problems of restricting the safety evaluation of aristolochic acid compounds and the research on quality control methods of aristolochic acid series compounds

Inactive Publication Date: 2017-01-11
上海诗丹德标准技术服务有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the quality control index of Chinese medicinal materials containing aristolochic acid compounds reported in the literature is only the determination of aristolochic acid A, and no systematic quality control method research has been carried out for aristolochic acid compounds. The overall quality control research and safety evaluation of aristolochic acid compounds are limited

Method used

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  • Method for measuring content of aristolochic acid compound in traditional Chinese medicine
  • Method for measuring content of aristolochic acid compound in traditional Chinese medicine
  • Method for measuring content of aristolochic acid compound in traditional Chinese medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Determination of Aristolochic Acid A, Aristolochic Acid B, Aristolochic Acid C and Aristolochic Acid D in Aristolochia Medicinal Materials

[0046] 1-1: Experimental equipment and reagents:

[0047] Agilent1200 high performance liquid chromatograph equipped with quaternary pump, online vacuum degasser, diode array detector and column thermostat.

[0048] Methanol, acetonitrile (chromatographically pure, purchased from Honeywell Company); Methanol (analytical pure, purchased from Sinopharm Chemical Reagent Co., Ltd.); self-made ultrapure water.

[0049] Aristolochic acid A (AAⅠcontent 97%); Aristolochic acid B (AAⅢ content 95.94%); Aristolochic acid C (AAⅢ content 96.1%); Aristolochic acid D (AAⅣa content 97.1%) Shidande Biotechnology Co., Ltd;

[0050] Aristolochic acid medicinal materials, MDL01-MDL09 were purchased from Anhui Bozhou Medicinal Pieces Market and Hebei Anguo Medicinal Pieces Market.

[0051] 1-2: Chromatographic conditions:

[0052] Chromatographic c...

Embodiment 2

[0073] Determination of Aristolochic Acid A, Aristolochic Acid B, Aristolochic Acid C and Aristolochic Acid D in Qingmuxiang

[0074] 1-1: Experimental equipment and reagents:

[0075] Agilent1200 high performance liquid chromatograph equipped with quaternary pump, online vacuum degasser, diode array detector and column thermostat.

[0076] Methanol, acetonitrile (chromatographically pure, purchased from Honeywell Company); Methanol (analytical pure, purchased from Sinopharm Chemical Reagent Co., Ltd.); self-made ultrapure water.

[0077] Aristolochic acid A (AAⅠcontent 97%); Aristolochic acid B (AAⅢ content 95.94%); Aristolochic acid C (AAⅢ content 96.1%); Aristolochic acid D (AAⅣa content 97.1%) Shidande Biotechnology Co., Ltd;

[0078] The medicinal material of Qingmuxiang was purchased from the market of medicinal herbs and decoction pieces in Bozhou, Anhui.

[0079] 1-2: Chromatographic conditions:

[0080] Chromatographic column: Agilent proshell EC-C18 4.6*50mm 2.7u...

Embodiment 3

[0100] Determination of Aristolochic Acid A, Aristolochic Acid B, Aristolochic Acid C and Aristolochic Acid D in Tianxian Teng

[0101] 1-1: Experimental equipment and reagents:

[0102] Agilent1200 high performance liquid chromatograph equipped with quaternary pump, online vacuum degasser, diode array detector and column thermostat.

[0103] Methanol, acetonitrile (chromatographically pure, purchased from Honeywell Company); Methanol (analytical pure, purchased from Sinopharm Chemical Reagent Co., Ltd.); self-made ultrapure water.

[0104] Aristolochic acid A (AAⅠcontent 97%); Aristolochic acid B (AAⅢ content 95.94%); Aristolochic acid C (AAⅢ content 96.1%); Aristolochic acid D (AAⅣa content 97.1%) Shidande Biotechnology Co., Ltd;

[0105] Tianxianteng medicinal material was purchased from the market of medicinal herbs and decoction pieces in Bozhou, Anhui Province.

[0106] 1-2: Chromatographic conditions:

[0107] Chromatographic column: Agilent proshell EC-C18 4.6*50mm...

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Abstract

The invention belongs to the field of biomedicine and particularly relates to a method for measuring the content of an aristolochic acid compound in traditional Chinese medicine. Contents of aristolochic acid A, aristolochic acid B, aristolochic acid C and aristolochic acid D in aristolochic acid traditional Chinese medicine can be measured at the same time, quality evaluation of the traditional Chinese medicine containing the aristolochic acid compound can be completed, and scientific bases can also be provided for safety evaluation of the aristolochic acid compound.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a method for determining the content of aristolochic acid compounds in Chinese medicinal materials. Background technique [0002] Aristolochic acid compounds are a class of nitrophenanthrene carboxylic acids, which naturally exist in Aristolochiaceae plants such as Aristolochia and Asarum, among which such as Guangfangji, Aoki Medicinal materials such as Xiang, Tianxianteng, Aristolochia, Xugufeng, and Zhushalian are still included in the 2015 edition of the Chinese Pharmacopoeia. Aristolochic acid compounds have been found to have anti-cancer and anti-infection activities, and can be used for bronchitis, tonsils, etc. Inflammation, nephritis, prostatitis and other infectious diseases, but now aristolochic acids have been confirmed to have clear nephrotoxicity, carcinogenicity, and teratogenicity. Improper use of drugs can produce strong toxic reactions, endangering the liv...

Claims

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Application Information

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IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 杨洲辛振强刘倩许纪锋夏梅玲谢天培钱勇
Owner 上海诗丹德标准技术服务有限公司
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