Method for inducing pinellia ternate callus and special culture medium of method

Abstract

The invention discloses a method for inducing pinellia ternate callus and a special culture medium of the method, and belongs to the technical field of plant tissue culture. According to the method, pinellia ternate explants are placed on an induction culture medium to be subjected to induction culture, calluses which grow rapidly and are vigorous are obtained, an MS culture medium serves as a basic culture medium of the induction culture medium, and 6-BA, NAA and cinnamomum longepaniculatum essential oil are added into the induction culture medium. The stable and efficient induction method of the pinellia ternate callus is established, the yield and quality of the callus are improved, the callus is normal in subculture proliferation, and important technical support is provided for further development of pinellia ternate breeding research.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and more specifically relates to a method for inducing pinellia callus and a special culture medium thereof. Background technique [0002] Pinellia belongs to the genus Pinellia of the family Araceae. It is a perennial herb, also known as wild taro, triple jump, etc. It is recorded in the treasure house of traditional Chinese medicine as an important Chinese medicinal material, and its origin is in my country and Japan. Pinellia is used as medicine after the tubers are dried. Pinellia is mainly distributed in Yunnan, Jiangnan, Sichuan, Guizhou, Hubei and other places in China. According to survey statistics, among the 558 traditional Chinese medicine prescriptions, Pinellia is the 22nd most frequently used. [0003] The sandy soil of Pinellia is generally excavated in summer and autumn, and the tubers are spherical or oblate. It is a traditional Chinese medicinal material that is ...

AI Technical Summary

Problems solved by technology

But despite this, the "Shui Pinellia" that was seized still became popular instead of Pinellia. In addition, due to the particularity of pinellia reproduction, a large amount of seeds is required for its tuber reproduction, especially in continuous cropping, it is very easy to cause virus infection, and the disease is very serious during heavy cropping, and the quality and yield are greatly reduced, resulting in planting hysteresis

On the other hand, as far as the market itself is concerned, the demand for Chuan Pinellia is also growing continuously. The reality of the shortage of supply has led to the excessive use of "Water Pinellia" and cannot be contained, which greatly limits the development of the Sichuan Pinellia industry.

[0004] Although the artificial cultivation of pinellia can achieve partial success, if you want to expand the planting, there are still problems with low reproductive coefficients: first, Pinellia is limited to bulbil propagation and tuber propagation, and its seed propagation method is different from the actual production process. Second, the pest control technology does not match the production, such as viral diseases, rot diseases, epidemic diseases, etc., have a great impact on production; third, the large-scale planting area is small; Fourth, it is more complicated to apply to actual production, and the cost of planting is high

However, this case has obvious shortcomings such as slow callus proliferation and low organ differentiation efficiency.

In addition, there are many limiting factors in the selection of explants in this case. The tubers and plant buds used as explants have the problems of incomplete disinfection and high pollution rate; and the seedling time is too long to meet the market demand.

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