Fermentation culture method of trichoderma and trichoderma agent

A technology of fermentation culture and Trichoderma agent, applied in the field of Trichoderma fermentation and Trichoderma agent, can solve the problem of high material cost and achieve the effects of low cost, good propagation effect and low cost

Pending Publication Date: 2022-01-04
LANZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the cost of materials used to multiply Trichoderma is relatively high at present, so I want to find a cheap propagation method to provide technical means for better utilization of Trichoderma

Method used

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  • Fermentation culture method of trichoderma and trichoderma agent
  • Fermentation culture method of trichoderma and trichoderma agent

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preparation example Construction

[0060] In a preferred embodiment, a typical medium formula for cultivating biocontrol Trichoderma, the preparation method of Trichoderma agent, comprises the following steps:

[0061] 1) Seed culture of Trichoderma species: Trichoderma species is Trichoderma harzianum; Trichoderma spores are inoculated in PDA medium, the culture temperature is 25-30°C, and the light conditions are 8-16 hours of light and 8-16 hours of darkness Alternately, the culture time is 4 days;

[0062] 2) Dilute the Trichoderma spores obtained in step 1) with sterile water to be a Trichoderma spore suspension, and the dilution concentration is 1×10 3 cfu / ml-1×10 8 cfu / ml, the dilution method is hemocytometer counting, and adjust the pH to 4-7;

[0063] 3) The Trichoderma spore suspension that step 2) is obtained is carried out liquid state fermentation, and fermentation medium is potato starch processing waste nutrient solution, and inoculum size is by volume ratio Trichoderma spore suspension: potato...

Embodiment 1

[0070] A method for fermenting and cultivating Trichoderma, comprising the following steps:

[0071] 1) Seed culture of Trichoderma species: Trichoderma species is Trichoderma harzianum; Trichoderma spores are inoculated in PDA medium, the culture temperature is 25°C, the light conditions are 8 hours of light and 16 hours of darkness alternately, and the culture time is 4 sky;

[0072] 2) Dilute the Trichoderma spores obtained in step 1) with sterile water to be a Trichoderma spore suspension, and the dilution concentration is 1×10 3 cfu / ml, the dilution method is counted on a hemocytometer, and the pH is adjusted to 4;

[0073] 3) the Trichoderma spore suspension that step 2) is obtained is carried out liquid state fermentation, and liquid fermentation medium is potato starch processing waste nutrient solution, and inoculum size is by volume ratio Trichoderma spore suspension: potato starch processing waste nutrient solution is 1: 70, the culture condition is 25°C, shaker c...

Embodiment 2

[0076] A method for fermenting and cultivating Trichoderma, comprising the following steps:

[0077] 1) Seed culture of Trichoderma species: Trichoderma species is Trichoderma longibrachiae; Trichoderma spores are inoculated in PDA medium, the culture temperature is 25°C, the light conditions are 8 hours of light and 16 hours of darkness alternately, and the culture time is 4 days;

[0078] 2) Dilute the Trichoderma spores obtained in step 1) with sterile water to be a Trichoderma spore suspension, and the dilution concentration is 1×10 3 cfu / ml, the dilution method is counted on a hemocytometer, and the pH is adjusted to 4;

[0079] 3) the Trichoderma spore suspension that step 2) obtains is carried out liquid state fermentation, and the liquid fermentation culture medium is potato starch processing waste nutrient solution, and inoculum size is by volume ratio Trichoderma spore suspension: potato starch processing waste nutrient solution is 1: 70, the culture condition is 2...

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Abstract

The invention provides a fermentation culture method of trichoderma and a trichoderma agent, and relates to the technical field of microbial fermentation culture, the fermentation culture method of trichoderma comprises the following steps: performing liquid fermentation culture on a trichoderma spore suspension to obtain a trichoderma liquid fermentation liquid, and performing solid fermentation propagation culture to obtain the trichoderma agent; adopting a potato starch processing waste culture solution for liquid fermentation culture; and adopting a leaf solid culture medium for solid fermentation propagation culture, wherein the leaf solid culture medium comprises 10-20 parts by weight of lawn grass leaves, 10-20 parts by weight of clover leaves, 10-20 parts by weight of paulownia leaves, 10-20 parts by weight of locust leaves, 10-20 parts by weight of poplar leaves, 10-20 parts by weight of cypress leaves and 10-20 parts by weight of glucose. The specific culture mediums are selected for liquid fermentation culture and solid fermentation culture in sequence, the propagation efficiency of the trichoderma is improved, the spore yield is large, and the cost is low.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation and cultivation, in particular to a method for fermentation and cultivation of Trichoderma and a Trichoderma agent. Background technique [0002] Trichoderma (Trichoderma Pers.Fr.) fungi belong to Deuteromycotina (Deuteromycotina), Hyphomycetes (Hyphomycetes), Hyphomycetales (Hyphomycetales) Myxosporae (Gloiosporae), and its sexual stage is Ascomycetes Hypocrea of ​​the subphylum Ascomycotina, order Hypocreales, family Hypocreaceae. Trichoderma can produce a variety of enzymes, including cellulase, hemicellulase, chitinase, xylanase and protease, etc. These enzymes are widely used in food, hygiene, feed, pulping, textile, Fields such as papermaking, environmental protection and biological genetic engineering are therefore important industrial enzyme production bacteria, such as Trichoderma reesei (T.reesei=H.jecorina) is a cellulase production bacteria; some Trichoderma produce var...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12R1/885
CPCC12N1/14
Inventor 古丽君冯琦胜吴淑娟
Owner LANZHOU UNIVERSITY
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