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Molecular marker, primer and method for identifying lota lota in Heilongjiang river basin and lota lota in Irtysh river basin and application

A molecular marker and watershed technology, applied in the field of molecular genetics of aquatic animals, can solve the problems of mixed wild germplasm, unknown source of burbot multiplication and release parents, and achieve the effect of avoiding inaccuracy

Pending Publication Date: 2022-01-14
HEILONGJIANG RIVER FISHERY RES INST CHINESE ACADEMY OF FISHERIES SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to avoid the unknown source of burbot breeding and releasing parents in the process of breeding and releasing, which will lead to the mixing of wild germplasm in the water systems of the two watersheds, it is urgent to establish a method for quickly, accurately and effectively distinguishing the germplasm resources of burbot in the two watersheds

Method used

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  • Molecular marker, primer and method for identifying lota lota in Heilongjiang river basin and lota lota in Irtysh river basin and application
  • Molecular marker, primer and method for identifying lota lota in Heilongjiang river basin and lota lota in Irtysh river basin and application

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Embodiment 1

[0024] Embodiment 1. identify the method of Heilongjiang Basin burbot and Irtysh River Basin burbot

[0025] 1. The gene sequence is mitochondrial gene

[0026] The sequence of burbot in the Heilongjiang River Basin is shown as SEQ ID NO:1.

[0027] TGTCCTCTCTATAGGAGCCGTCTTTGCTATCATAGCAGCTTTTGTTCACTGATTCCCGCTGTTTACAGGCTATACTCTCCACGACACCTGAACAAAAATCCACTTTGGGGT

[0028] The sequence of burbot in the Irtysh River Basin is shown as SEQ ID NO:2.

[0029] TGTCCTCTCTATAGGAGCCGTCTTTGCTATCATAGCAGCTTTTGTTCACTGATTCCCGCTGTTTACAGGCTATACTCTCCATGACACCTGAACAAAAATCCACTTTGGGGT

[0030] Primers were designed according to the mitochondrial genes of burbot in the Heilongjiang River Basin and burbot in the Irtysh River Basin:

[0031] LLC1-3F: 5'-TGTCCTTCCTATAGGAGCCGT-3' as SEQ ID NO:3

[0032] LLC1-3R: 5'-ACCCCAAAGTGGATTTTTGTTCA-3' as SEQ ID NO:4

[0033] 2. Identification method

[0034] The experimental samples selected in this embodiment are burbot in the Heilongjiang River Basin and burb...

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Abstract

The invention relates to a molecular marker, a primer and a method for identifying lota lota in Heilongjiang river basin and lota lota in Irtysh river basin and application, and belongs to molecular genetics of aquatic animals. The nucleotide sequence of the SNP molecular marker for identifying Lota lota in the Heilongjiang river basin and Lota lota in the Irtysh river basin is SEQ ID NO: 1 or SEQ ID NO: 2, and the nucleotide sequences of the primer are SEQ ID NO: 3 and SEQ ID NO: 4. According to the method for identifying the lota lota in the Heilongjiang river basin and the lota lota in the Irtysh river basin on the gene level, the problem that the lota lota is difficult to identify and distinguish due to difficulty in distinguishing appearance forms, indefinite background sources, damage to processing forms and the like is effectively solved, and the method has the advantages of simplicity, convenience, rapidness, high efficiency and accuracy.

Description

technical field [0001] The invention belongs to the technical field of molecular genetics of aquatic animals, and in particular relates to a molecular marker, a primer, a method and an application for identifying burbot in the Heilongjiang River Basin and burbot in the Irtysh River Basin. Background technique [0002] Burbot (Lota lota) belongs to Codformes Gadiformes, Cod suborder Gadoidae, Cod family Gadidae, burbot genus Lota, commonly known as mountain catfish. It is mainly distributed in cold-water rivers and lakes at high latitudes in the northern hemisphere. It is the only species in the cod family that lives in fresh water. In my country, it is mainly distributed in the Heilongjiang River Basin and the Irtysh River Basin. Burbot is a large fish with delicate meat, no intermuscular spiny protein and rich in lipids. Its liver is relatively large (accounting for 6-9% of body weight), and it can be used to make cod liver oil. It is an important cold-water economic fish. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12Q1/6858C12N15/11
CPCC12Q1/6888C12Q1/6858C12Q2600/124C12Q2600/156C12Q2531/113C12Q2565/125
Inventor 栾培贤户国董晓丽张晓峰
Owner HEILONGJIANG RIVER FISHERY RES INST CHINESE ACADEMY OF FISHERIES SCI