Bionic leaf model and preparation method and application thereof

Abstract

The invention belongs to the technical field of micro-fluidic chip manufacturing. The invention provides a bionic leaf model and a preparation method and application thereof. The preparation method of the model comprises the following steps that a fresh leaf is placed in an ethanol solution for disinfection, and the back side of the leaf is taken as a male mold; a glass plate is adhered with double-sided adhesive tape, the front side of the leaf is adhered with the double-sided adhesive tape, the surface of the male mold is covered with a layer of PDMS polymer, vacuumizing is conducted to remove bubbles, and then the curing treatment is conducted to obtain a female mold of the bionic leaf model; the modification liquid is placed in a container, then the female mold of the bionic leaf model is suspended on the upper side of the modification liquid, standing is conducted after the container is sealed, the modification liquid is evaporated, and an isolation layer is formed on the surface of the female mold of the bionic leaf model; and the PDMS polymer is poured into the modified female mold of the bionic leaf model, vacuumizing is conducted to remove the bubbles, and then the curing treatment is conducted to obtain the bionic leaf model. The bionic leaf model can get rid of seasonal limitation and is convenient for experiments; reutilization is achieved, and the environment is protected; and the variable can be controlled.

Description

technical field [0001] The invention relates to the field of microfluidic chip production, in particular to a bionic leaf model and its preparation method and application. Background technique [0002] The venation sequence of fresh leaves is intricate, and the microfluidic chip made by using this venation sequence can simulate and observe the flow of components in blood vessels in the human body, which can be used in medical research. [0003] However, the general microfluidic chip fabrication has certain limitations: 1) Since the venation on the back of the leaf is used as the positive mold, there are seasonal restrictions on the leaves, which affects the fabrication of the chip and subsequent experiments. [0004] 2) Frequent picking of leaves will cause certain damage to the environment. [0005] 3) A unified microfluidic chip can be produced by using the template, and the shape used in subsequent experiments is consistent and variables are controlled. [0006] 4) The ...

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Problems solved by technology

[0003] However, the production of general microfluidic chips has certain limitations: 1) Since the venation on the back of the leaves is used as the positive mold, there are seasonal restrictions on the leaves, which affects the production of the chip and the subsequent experiments.

[0004] 2) Frequent picking of leaves will cause certain damage to the environment

At present, there are no reports related to the bionic leaf model

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