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Iodine resistance screening kit for papillary thyroid carcinoma

A thyroid cancer and kit technology, applied in the field of cancer diagnosis, can solve the problems of tissue and organ metastasis, ineffective radioactive iodine 131 treatment, affecting the quality of life and survival rate of patients

Pending Publication Date: 2022-01-18
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, as the disease progresses, 6-20% of PTC will gradually metastasize to tissues and organs and then develop iodine resistance in papillary thyroid carcinoma (referred to as "PTC iodine resistance"), that is, radioactive iodine 131 treatment is ineffective
PTC iodine resistance seriously affects the quality of life and survival rate of patients
However, there is no report on the relationship between miR-15a-5p and iodine resistance in PTC

Method used

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  • Iodine resistance screening kit for papillary thyroid carcinoma
  • Iodine resistance screening kit for papillary thyroid carcinoma
  • Iodine resistance screening kit for papillary thyroid carcinoma

Examples

Experimental program
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Embodiment 1

[0024] Embodiment 1: kit of the present invention

[0025] This embodiment takes the PCR screening kit as an example for introduction.

[0026] 1. Composition of the kit

[0027] 1.1 Reverse transcription reagents

[0028] (1) MicroRNA Stem-loop reverse transcription primer

[0029] The sequence (SEQ ID NO.1) is:

[0030] 5'-GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCACAAA-3' (SEQ ID NO. 1).

[0031] (2) Other conventional reagents required for reverse transcription

[0032] Including dNTP, 5×buffer, M-MLV reverse transcriptase; it can be a conventional reverse transcription reagent in the field.

[0033] 1.2 Real-time fluorescent quantitative PCR reagents

[0034] (1) Quantitative PCR primers

[0035] The sequences are:

[0036] Forward primer (SEQ ID NO.2): 5'-GGGTAGCAGCACATAATGG-3'(SEQ ID NO.2);

[0037] Reverse primer (SEQ ID NO. 3): 5'-CTCAACTGGTGTCGTGGA-3' (SEQ ID NO. 3).

[0038] (2) Real-time fluorescent quantitative PCR master mix

[0039] TB Green Premix...

experiment example 1

[0057] Experimental Example 1: Clinical Verification

[0058] In this experiment example, the level of exosomal miR-15a-5p in clinical plasma samples was detected, and the levels of miR-500a-5p, miR-1296-5p and miR-3120-5p were used as controls. Among them, miR-500a-5p, miR-1296-5p and miR-3120-5p are miRNAs previously reported to be associated with sodium iodide transporter (SLC5A5, NIS) and tumors.

[0059] 1. Method

[0060] Randomly select 21 plasma samples of iodine-tolerant PTC patients and 42 plasma samples of non-iodine-resistant PTC patients, use the exosome isolation kit ExoQuick-TCTM to extract exosome RNA, use the kit in Example 1 and Methods The level of miR-15a-5p in plasma exosomes was detected, and the primers in Example 1 were replaced with primers of miR-500a-5p, miR-1296-5p and miR-3120-5p to detect miR in plasma exosomes - Levels of 500a-5p, miR-1296-5p and miR-3120-5p. Then the external reference Cel-miR-39 was used to quantify the aforementioned miRNAs. ...

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Abstract

The invention discloses application of a reagent for detecting exosome miR-15a-5p in preparation of a papillary thyroid carcinoma iodine resistance screening kit, and belongs to the field of cancer diagnosis. In the plasma exosome of a patient with iodine-resistant papillary thyroid carcinoma, the level of miR-15a-5p is obviously lower than that of a patient with non-iodine-resistant papillary thyroid carcinoma. According to the discovery, the reagent for detecting the exosome miR-15a-5p is used for preparing the papillary thyroid carcinoma iodine resistance screening kit and has a good application prospect.

Description

technical field [0001] The invention belongs to the field of cancer diagnosis. Background technique [0002] Papillary thyroid carcinoma (papillary thyroid carcinoma, PTC) is the most common pathological subtype of thyroid cancer. The 10-year survival rate of PTC can be as high as 90% with traditional treatment methods (surgery, TSH suppression and radioactive iodine 131). However, as the disease progresses, 6-20% of PTC will gradually metastasize to tissues and organs, and then develop iodine resistance in papillary thyroid carcinoma (referred to as "PTC iodine resistance"), that is, radioactive iodine 131 treatment is ineffective. PTC iodine resistance seriously affects the quality of life and survival rate of patients. Studies have shown that the 10-year survival rate of patients with PTC metastases who took radioactive iodine was about 56%, while the 10-year survival rate of radioactive iodine-resistant patients was only 10%. Early identification of patients with PTC i...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886
CPCC12Q1/6886C12Q2600/158C12Q2600/178
Inventor 雷建勇李根棚陈文杰朱精强李志辉魏涛
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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