Construction method of corpus callosum cut-off animal model
A technology of animal models and construction methods, which is applied in the fields of medical science, veterinary instruments, veterinary surgery, etc., can solve the problems of difficult operation, less animal models, and more venous sinus bleeding, etc. The effect of less damage and less bleeding
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[0018] Construction method of animal model of corpus callosum section
[0019] After the mouse was anesthetized with 1.5% pentobarbital sodium, the brain was fixed on a stereotaxic instrument, the skin on the top of the skull was prepared, disinfected with iodine, the skin was incised at the midline of the top with a scalpel, and the skin on both sides was fixed with skin clips to expose the top skull. Use a marker pen to draw a straight line parallel to the midline at 0.5 mm to the left of the midline of the skull ( figure 1 shown), anteriorly to 2mm anterior to the sagittal suture, and posteriorly to 2.7mm posterior to the sagittal suture. Use a micro-drill to cut a bone suture parallel to the midline along the drawn line. The depth is just to penetrate the periosteum, about 0.6mm. There is a sense of void when penetrating, and the pia mater and brain parenchyma should not be injured. With a tiny cotton ball soaked in saline, wipe away bone debris.
[0020] The mouse skull...
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