A kind of polypeptide analog and its application

A drug and composition technology, applied in the direction of polypeptides, peptides, peptide sources, etc. containing localization/targeting motifs, to achieve the effects of inhibiting inflammatory response, improving inflammatory damage, and promoting the proliferation of skin surface cells

Active Publication Date: 2022-07-08
NANTONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no research report on the use of peptide analogs to treat the inflammatory response of frostbite

Method used

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  • A kind of polypeptide analog and its application
  • A kind of polypeptide analog and its application
  • A kind of polypeptide analog and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A polypeptide analog, wherein the polypeptide in the polypeptide analog is Tat-Sam68; wherein, Tat is a transmembrane structure sequence of 11 amino acids; Sam68 is a 15 amino acid fragment on the Sam68 protein sequence with the highest affinity with MD2.

[0024] The polypeptide analogs include: amino acid fragments at positions 47-57 in the transcriptional transactivator protein Tat, and amino acid fragments at positions 281-295 in the protein sequence of Sam68.

[0025] The 47-57th amino acid fragment in the transcriptional transactivator protein Tat is located at the N-terminus of the polypeptide analog, and the 281-295th amino acid fragment on the Sam68 protein sequence is located at the C-terminus of the polypeptide analog .

[0026] The transcriptional transactivator protein Tat can enter the cell through transmembrane and has a strong internalization effect; The amino acid fragment at positions 47-57 is shown in SEQ ID NO. 1, abbreviated as YGRKKRRQRRR, specifi...

Embodiment 2

[0031] The application of the polypeptide analog described in Example 1 in the intervention and treatment of frostbite medicine, the specific steps are as follows:

[0032] 1. To study the changes of mRNA expression of MD2 and TNF-α in the skin tissue of the frostbite area after different time of frostbite on the mouse model of frostbite.

[0033] Based on the possible relationship between serum MD2 content and frostbite time after frostbite, the experimental study firstly constructed a mouse back skin tissue frostbite model, and detected the changes of related indicators after frostbite at different times.

[0034] The whole experiment was divided into four groups: the control group simulated by atmospheric pressure and oxygen, the frostbite 1 day group (model group), the frostbite 3 days group (model group), and the frostbite 7 days group (model group).

[0035] The frostbite mouse model is specifically: after removing the hair on the back of the neck of the ICR mouse, a sta...

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PUM

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Abstract

The invention discloses a polypeptide analog and an application thereof. The polypeptide in the polypeptide analog is Tat-Sam68, and its amino acid sequence is shown in SEQ ID NO. 3; wherein, Tat is a membrane-penetrating structure sequence of 11 amino acids, Specifically as shown in SEQ ID NO.1; Sam68 is a 15 amino acid fragment on the Sam68 protein sequence with the highest affinity with MD2, as shown in SEQ ID NO.2. The polypeptide analog can be used in the preparation of a drug for intervening and treating frostbite. The polypeptide analogs of the present invention can specifically target MD2 and bind to endogenous MD2, thereby inhibiting MD2-mediated cell damage induced by low temperature, and is expected to improve frostbite.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, relates to the technical field of frostbite medicines, and in particular relates to a polypeptide analog and an application thereof. Background technique [0002] Frostbite or high-altitude cold injury is a common disease in cold and high-altitude regions in my country. It can occur in daily life and occupational production activities, and the morbidity and mortality rates are high. The study and proposal of frostbite treatment programs can not only help people working in high altitudes, It can also provide medical support and treatment methods for military drills and combat readiness personnel. [0003] Frostbite or hypothermia is caused by the continuous loss of heat from the human body after exposure to high altitude or cold, and the core body temperature gradually decreases to below 35°C. The cellular mechanism of frostbite is currently believed to be mainly due to the release of various ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K19/00A61K38/17A61P17/02
CPCC07K14/435A61P17/02C07K2319/03A61K38/00Y02A50/30
Inventor 骆倩倩杨万萍姜辉王国华陈忠平朱俐
Owner NANTONG UNIVERSITY
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