Method for simultaneously detecting diosmin and hesperidin in citrus flavone bulk drug

A technology of citrus flavonoids and diosmin, which is applied in the direction of measuring devices, material separation, and analysis of materials, can solve the problems of low detection efficiency, long detection time, complicated operation steps, etc., and achieve no impurity interference, rapid detection, detection high precision effect

Pending Publication Date: 2022-01-28
CHENGDU YAZHONG BIOPHARML
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  • Abstract
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Problems solved by technology

Although this method solves the problem of long detection time, the operation steps are complicated and the detection efficiency is low

Method used

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  • Method for simultaneously detecting diosmin and hesperidin in citrus flavone bulk drug
  • Method for simultaneously detecting diosmin and hesperidin in citrus flavone bulk drug
  • Method for simultaneously detecting diosmin and hesperidin in citrus flavone bulk drug

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Embodiment

[0030] 1. Experimental equipment

[0031] Agilent 1260 high performance liquid chromatograph; chromatographic column EC 100 / 4.6NUCLEOSIL 100-3C18; electronic balance XSR205DU one hundred thousandth.

[0032] 2. Reagents

[0033] DMSO (dimethyl sulfoxide), methanol, acetonitrile, acetic acid;

[0034] 89.0% diosmin, Chengdu Yazhong Biopharmaceutical Co., Ltd., batch number WS4003-210401;

[0035] 90.5% hesperidin, Mianyang Diao Pharmaceutical Co., Ltd., batch number WS1009-201001;

[0036] 89.6% hesperidin, Chengdu Yazhong Biopharmaceutical Co., Ltd., batch number WS1009-210601;

[0037] Citrus flavonoid raw materials, Chengdu Yazhong Biopharmaceutical Co., Ltd., batch numbers 4002-S210501, 4002-S210502, 4002-S210503.

[0038] 3. HPLC determination

[0039] Preparation of reference substances: Accurately weigh 25 mg of diosmin reference substance and 12.5 mg of hesperidin reference substance in the same 25ml volumetric flask, add DMSO to dissolve and constant volume to obt...

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Abstract

The invention discloses a method for simultaneously detecting diosmin and hesperidin in a citrus flavone bulk drug. The method comprises the following steps of respectively preparing a test solution and a mixed reference solution of diosmin and hesperidin; determining the solution according to a high performance liquid chromatography, wherein the determination conditions are that a chromatographic column is C18, the mobile phases comprise a mobile phase A and a mobile phase B of methanol, and the ratio of acetonitrile to glacial acetic acid to methanol to water is 2: 6: 24: 70; gradient elution is carried out, the flow rate is 1ml / min, the column temperature is 40 DEG C, and the wavelength is 275nm. The detection method can be used for simultaneously detecting the main components (diosmin and hesperidin) of the citrus flavone raw material medicine, and meanwhile, impurities in the citrus flavone cannot cause interference to detection.

Description

technical field [0001] The invention is a method for simultaneously detecting diosmin and hesperidin in citrus flavonoid raw materials, in particular to a method for efficiently detecting diosmin and hesperidin in citrus flavonoid raw materials by using HPLC method, which belongs to drug detection technology field. Background technique [0002] Citrus Flavone Tablets (Aimilang) is approved by the State Drug Administration for the treatment of symptoms related to venous lymphatic insufficiency such as heavy legs, pain, morning soreness and discomfort, and acute hemorrhoid attacks, and has been approved by China A compound preparation recommended in Guidelines for the Diagnosis and Treatment of Chronic Venous Diseases. According to its instructions, the main ingredients of Citrus Flavonoid Tablets are diosmin and hesperidin, and the specifications are 450mg and 50mg. The existing domestic similar varieties are diosmin tablets (Getai, Mayinglong), all of which are unilateral ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/86
CPCG01N30/02G01N30/06G01N30/8679
Inventor 肖国庆王江南许志强
Owner CHENGDU YAZHONG BIOPHARML
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