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Cis-platinum cross-linked protein hydrogel and preparation method thereof

A technology of cross-linking protein and protein water, which is applied to medical preparations with non-active ingredients, medical preparations containing active ingredients, and pharmaceutical formulas. Degradability, good biocompatibility, easy preparation

Active Publication Date: 2022-02-01
HANGZHOU NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to its large toxic and side effects, it often causes huge psychological and physical harm to patients.

Method used

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  • Cis-platinum cross-linked protein hydrogel and preparation method thereof
  • Cis-platinum cross-linked protein hydrogel and preparation method thereof
  • Cis-platinum cross-linked protein hydrogel and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Add 300mg of bovine serum albumin powder into 700μL of ultrapure water, heat it in a water bath at 45°C to dissolve it completely to obtain a light yellow clear solution, add 15mg of cisplatin to the solution and put it into a shaker, set the temperature at 42°C, and rotate at 230r / min , or using mechanical stirring to uniformly disperse cisplatin in the solution, and after 36-48h of reaction, a protein hydrogel with cisplatin as a cross-linking agent is obtained.

[0038] The cisplatin cross-linked albumin hydrogel prepared in Example 1 of the present invention was quickly frozen by the liquid nitrogen quick freezing method, and freeze-dried to obtain a freeze-dried gel sample; the sample was subjected to a scanning electron microscope test to obtain a gel microscopic Form photo. From figure 2 It can be seen that the complex hydrogel material has a connected macroporous structure, which is conducive to the delivery and release of drugs in the gel material.

[0039] ...

Embodiment 2

[0043] Add 200mg of bovine serum albumin powder into 800μL of ultrapure water, heat it in a water bath at 45°C to dissolve it completely to obtain a light yellow clear solution, add 15mg of cisplatin to the solution and put it in a shaker, set the temperature at 10°C, and rotate at 230r / min , or using mechanical stirring to disperse cisplatin evenly in the solution, and after 72-84 hours of reaction, a protein hydrogel with cisplatin as a cross-linking agent is obtained.

[0044] In the process of preparing cisplatin cross-linked albumin hydrogel in Example 2 of the present invention, when cisplatin completely disappears, pour the fluid colloid from the container into a mold with a bottom diameter of 2.5 cm, and place it in a shaker , set the temperature at 45°C to continue to gel. After gel formation, the disc gel was taken out and placed on a rotational rheometer to test the modulus of the cisplatin-crosslinked albumin hydrogel.

[0045] To the cisplatin cross-linked albumi...

Embodiment 3

[0048] Add 60mg of bovine serum albumin powder into 940μL of ultrapure water, heat it in a water bath at 65°C to dissolve it completely to obtain a light yellow clear solution, add 10mg of cisplatin to the solution and put it into a shaker, set the temperature at 37°C, and rotate at 200r / min , or using mechanical stirring to disperse cisplatin evenly in the solution, and after 72-84 hours of reaction, a protein hydrogel with cisplatin as a cross-linking agent is obtained.

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Abstract

The invention discloses a cis-platinum cross-linked protein hydrogel and a preparation method thereof. The cisplatin cross-linked protein hydrogel is prepared from the following main components in percentage by mass: 0.5 to 5.0 percent of drug, 6.0 to 50.0 percent of serum albumin and 47.0 to 93.0 percent of solvent medium, wherein carboxyl on the surface of the serum albumin and a drug form a coordinate bond; and the drug is cis-platinum. The hydrogel preparation provided by the invention is simple in structure and easy to prepare, and the used cis-platinum has dual effects: the used cis-platinum is a cross-linking agent for promoting the formation of protein hydrogel and is also an anti-tumor drug for playing a tumor inhibition curative effect. Introduction of other components is effectively reduced, and the potential risk of body side effects is reduced; and on the other hand, the protein carboxyl group limits the release of the cis-platinum through the coordination effect, so that the burst release behavior of the medicine can be reduced to a great extent, and the reduction of the toxicity of the cis-platinum in the body tissue is facilitated.

Description

technical field [0001] The invention belongs to the field of pharmaceutical preparations, and in particular relates to a protein hydrogel with cisplatin as a cross-linking agent and a preparation method thereof. Background technique [0002] Due to the rapid growth of solid tumor tissue, it causes higher intercellular pressure compared with normal physiological tissue, which makes the effective drug concentration in the tumor area during chemotherapy low; in addition, the tumor microenvironment is very complex, which also affects drug diffusion. One of the important reasons. However, the method of intravenous injection has the disadvantages of large toxic and side effects, low effective drug concentration at the lesion site, and the like. Local tumor therapy has the advantages of concentrated drug concentration, less systemic side effects, and can overcome physiological barriers to drug delivery, so it has received extensive research and attention in recent years. [0003]...

Claims

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Application Information

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IPC IPC(8): A61K9/06A61K47/64A61K33/243A61K45/06A61P35/00
CPCA61K9/06A61K47/643A61K33/243A61K45/06A61P35/00A61K2300/00Y02A50/30A61K47/42
Inventor 于双江闫安魏舒孙鸿程刘俊秋
Owner HANGZHOU NORMAL UNIVERSITY
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