Multi-channel micro-fluidic chip for blood sample detection

Abstract

The invention discloses a multi-channel micro-fluidic chip for blood sample detection. The chip comprises a micro-fluidic chip body provided with a micro-channel, wherein the micro-channel is used for conveying liquid in the micro-channel to move along the micro-channel by adopting an electrowetting pipetting technology, the micro-fluidic chip body is also provided with a sample adding area and at least one measuring channel, the sample adding area communicates with the measuring channel through a transfer micro-channel, the measuring channel comprises a mixed blood sample detection area, the mixed blood sample detection area is used for placing a mixed blood sample and allowing a rear-end detection system to detect technical data of the mixed blood sample and generate a diagnosis result, and the mixed blood sample is liquid obtained by fully mixing a blood sample and a reagent. The micro-fluidic chip is combined with the electrowetting technology to detect the blood sample, a detection process is high in accuracy control, and each measuring channel only needs 10-20 microliters of the blood sample, so the usage amount of the blood sample is greatly reduced. Manual operation errors in a detection process are eliminated, and the phenomenon of detection errors existing between the measuring channels is avoided.

Description

technical field [0001] The invention relates to a microfluidic chip, in particular to a multi-channel microfluidic chip for blood sample detection. Background technique [0002] Blood testing technology refers to related technologies that use blood samples as objects for in vitro detection and diagnosis, involving various blood-related tests and analysis and diagnosis of various diseases. With the development of blood testing technology, it is possible to better judge human diseases and provide treatment guidance through blood testing. From the early invention of blood cell straws, blood cell counting, hemoglobin counting and blood cell separation and other blood testing related technologies; to the in-depth understanding of the coagulation mechanism in modern times, the emergence of four conventional coagulation detection technologies and the "thrombosis and hemostasis" The publication of the Waterfall Theory led to the emergence of a technique for measuring the entire pro...

AI Technical Summary

Problems solved by technology

[0004] 1. Large amount of blood samples for diagnosis: Since blood samples cannot be used accurately, when taking blood samples, excessive blood samples will be selected to ensure correct measurement, resulting in the disadvantage of excessive blood sample size

[0005] 2. There are errors in pre-processing samples by human operation: before the test on the machine, the quantitative extraction of blood samples and the mixing process with supporting reagents are all manually completed, which will lead to operations by different people and the same person at different times. Operation will bring error to the result

[0006] 3. There are errors between the measurement channels: due to the use of processed parts, which involve the process of installation and debugging, between two channels; due to material properties, installation differences, etc., the channel test environments are different, and there are errors in the test results

[0007] 4. There are few measurement channels, which are not suitable for mass inspection: the measurement channel adopts conventional processing structural parts, which are large in size and limited by the spatial layout of the measuring instrument. There cannot be too many measurement channels, which cannot meet the needs of mass inspection

Therefore, how to use the dielectric wetting technology combined with the microfluidic chip to realize the pretreatment and detection of sample blood has not been reported yet.

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