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Method for identifying purified water and natural mineral water based on endogenous active intermediate

An endogenous activity and intermediate technology, which is applied in the field of distinction and identification of natural mineral water, can solve the problems of low identification degree, low cost of counterfeiting and high detection cost, and achieve accurate identification method, high discrimination rate and anti-interference. strong effect

Pending Publication Date: 2022-02-08
杭州食疗晶元生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, whether it is pure water or natural mineral water, there are many brands and types, but there is no targeted and effective identification method between the two types of water.
The current identification methods include appearance identification, taste, refractive index, specific gravity test, alcohol addition test, elemental analysis, etc. These methods either have low identification degree, strong subjectivity, or high detection cost and low counterfeiting cost. Difficult to ensure success rate

Method used

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  • Method for identifying purified water and natural mineral water based on endogenous active intermediate
  • Method for identifying purified water and natural mineral water based on endogenous active intermediate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] For the optimization of the working solution concentration of fluorescent probe 2-amino-5-phenylethynylpyrazine, the following steps are included:

[0029] 1) Prepare 0.024, 0.24, and 2.4mol / L fluorescent probe 2-amino-5-phenylethynylpyrazine working solutions respectively. The solvent used is ultrapure water, and the working solution is stored in a -20°C refrigerator for later use;

[0030] 2) Take 950μL of the water sample to be tested (a certain brand of purified water and a certain brand of natural mineral water) in a 1.5mL centrifuge tube, then add 50μL of fluorescent probe working solution of different concentrations and receive the fluorescent signal value at the ATTO signal receiver , keep recording for 4 minutes;

[0031] 3) draw a kinetic curve for each fluorescent signal value obtained, and integrate and summarize;

[0032] 4) Observe the kinetic curve after induction (such as figure 1 , 2 Shown), select the optimal fluorescent probe detection concentratio...

Embodiment 2

[0035] The method for identifying pure water and natural mineral water based on endogenous active intermediates in water comprises the following steps:

[0036] 1) Take 700 μL of 20 different batches of pure water 1 and 20 different batches of natural mineral water 1 to be tested in a 1.5 mL centrifuge tube, add 20 μL of fluorescent probe working solution and receive fluorescence at the ATTO signal receiver signal value;

[0037] 2) Draw a kinetic curve for each fluorescent signal value obtained, and calculate the average value of the fluorescent signal;

[0038] 3) Integrate and summarize the data obtained in step 2, analyze the difference between pure water and natural mineral water and make a distinction.

[0039] water species Fluorescent signal value total identification accuracy Purified water 1 590.2±62.8 19 95％ Natural Mineral Water 1 5552.3±422.5 20 100％

[0040] The analysis results show that there is a significant difference bet...

Embodiment 3

[0042] The method for identifying pure water and natural mineral water based on endogenous active intermediates in water comprises the following steps:

[0043] 1) Take 980 μL of 20 different batches of pure water 2 and 20 different batches of natural mineral water 2 to be tested in a 1.5 mL centrifuge tube, add 80 μL of fluorescent probe working solution to receive fluorescence at the ATTO signal receiver signal value;

[0044] 2) Draw a kinetic curve for each fluorescent signal value obtained, and calculate the average value of the fluorescent signal;

[0045] 3) Integrate and summarize the data obtained in step 2, analyze the difference between pure water and natural mineral water and make a distinction.

[0046] water species Fluorescent signal value total identification accuracy Pure water 2 560.4±53.3 20 100％ Natural mineral water 2 6855.7±498.6 20 100％

[0047] The analysis results show that there is a significant difference between...

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Abstract

A method for identifying purified water and natural mineral water based on an endogenous active intermediate comprises the following steps: dissolving a fluorescent probe reagent in ultrapure water to prepare a working solution, and refrigerating; adding the fluorescent probe working solution into a drinking water sample to be detected, and measuring a fluorescence signal value by using an instrument; drawing a dynamic curve chart according to the obtained fluorescence signal values, and calculating a fluorescence signal average value; identifying the to-be-detected drinking water sample according to the difference between the average values of the fluorescence signals of the to-be-detected drinking water sample and the purified water and the natural mineral water; the fluorescent probe working solution being a 2-amino-5-phenylethynyl pyrazine solution or a derivative of the 2-amino-5-phenylethynyl pyrazine solution. The identification method is suitable for various brands of purified water (including packaged drinking water) and natural mineral water, is accurate, rapid, simple, convenient, efficient, high in discrimination rate and strong in anti-interference performance, and can effectively distinguish ion-blended purified water and natural mineral water.

Description

technical field [0001] The invention belongs to the field of drinking water identification, in particular to a method for distinguishing and identifying pure water and natural mineral water based on endogenous active intermediates. Background technique [0002] Pure water is usually drinking water purified from tap water through multiple processes including adsorption, filtration, ultrafiltration, etc. Except for artificial additions, it basically does not contain or contains less natural minerals and elements; while natural mineral water is in a specific The underground mineral water formed under geological conditions and natural environment and accumulated in special geological structure rock formations not only contains rich minerals and trace elements, but also has the characteristics of refreshing, refreshing and sweet. However, these characteristics of mineral water can be artificially imitated. Unscrupulous manufacturers often make profits by adulterating pure water a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6402
Inventor 余兆硕罗思浩柯李晶徐天豪孟祥宇高观祯汪惠勤周建武饶平凡
Owner 杭州食疗晶元生物科技有限公司