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Application of MIF inhibitor 4-IPP in preparation of drug for treating brain glioma

A 4-IPP, brain glioma technology, which is applied in the application field of preparing drugs for the treatment of brain glioma, can solve the problem that the average survival time of glioma patients is not substantially improved, and achieves inhibition of glioma cells. The effect of proliferation and treatment of glioma

Pending Publication Date: 2022-02-11
郑琳
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the average survival time of glioma patients has not been substantially improved due to postoperative residual tumors, decreased radiosensitivity, and chemotherapy resistance.

Method used

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  • Application of MIF inhibitor 4-IPP in preparation of drug for treating brain glioma
  • Application of MIF inhibitor 4-IPP in preparation of drug for treating brain glioma
  • Application of MIF inhibitor 4-IPP in preparation of drug for treating brain glioma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] 1. Experimental method:

[0017] 1. Cell proliferation experiment CCK-8:

[0018] The first-generation U87 and U251 cells were seeded in 96-well plates at 2000 cells / well, and after 12 hours, the concentrations of 0.390625 μM, 0.78125 μM, 1.5625 μM, 3.125 μM, 6.25 μM, 12.5 μM, 25 μM, 50 μM, and 100 μM were added respectively. and 200 μM 4-IPP solution. After culturing for 24 hours, add 20 μL CCK-8 kit and incubate at 37°C for 2 hours, then use a fluorescent microplate reader to detect the OD value.

[0019] 2. Colony formation experiment:

[0020] Take the monolayer cultured cells in the logarithmic growth phase, trypsinize and pipette into single cells, resuspend and count the cells, spread them in a six-well plate at a density of 200 cells / well, shake the plate to disperse the cells, and place them in an incubator overnight. On the second day, 12.5 μM, 25 μM and 50 μM 4-IPP were added respectively, the medium was changed twice a week, and cultured for 2 to 3 weeks...

Embodiment 2

[0024] 1. Experimental method:

[0025] 1. Migration and Invasion experiments:

[0026] Transwell chambers (24-well plates) are used for Migration experiments and Matrigel-coated Invasion experiments. For the Migration experiment, take 200ul cell suspension (density 2×10 5 ) into the Transwell chamber, 500 μl of complete medium containing different concentrations of 4-IPP was placed in the lower chamber, and the samples were collected after 24 hours of incubation. For the invasion assay, 200ul of cell suspension (density 2×10 5 ), and at the same time, 500 μl of complete medium containing different concentrations of 4-IPP was added to the lower chamber, and samples were collected after 24 hours of incubation. After collection, the samples were stained with crystal violet and photographed under a microscope.

[0027] 2. Experimental results:

[0028] Figure 4 Migration experiments and Invasion experiments were performed in vitro to verify the inhibitory effect of the MIF...

Embodiment 3

[0030] 1. Experimental method:

[0031] 1. Establishment of subcutaneous heterotopic transplantation tumor model in male nude mice:

[0032] The 4-week-old male nude mice were kept in an SPF grade animal room according to 5 per cage, controlled appropriate temperature, humidity and light cycle, and provided sufficient mouse food and water. will total 5×10 7 U87 stable cells were injected into the right lower dorsal side (n=5 per group).

[0033] 2. Grouping: After one week, we randomly divided the mice into two groups. Intraperitoneal injection of PBS group and 20mg / kg4-IPP group respectively. Three weeks after the injection every other day, the mice were euthanized, and the tumors were removed and photographed.

[0034] 3. Preparation of injection: DMSO was used to dissolve 4-IPP first, and then the DMSO mother solution was dissolved in PBS to prepare 4-IPP injections with different concentrations.

[0035] 2. Experimental results:

[0036] like Figure 5 It is the eff...

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Abstract

The invention discloses an application of an MIF inhibitor 4-IPP in preparation of a drug for treating brain glioma. The invention determines that the MIF inhibitor 4-IPP can inhibit the proliferation, clone formation and metastasis ability of glioma cells, and the small molecule compound can inhibit the growth of subcutaneous ectopic transplantation tumors and has the effect of treating glioma. According to the tumor inhibition effect of the small molecule compound on glioma, the small molecule compound has application value in glioma treatment.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to the application of MIF inhibitor 4-IPP in the preparation of medicaments for treating brain glioma. Background technique [0002] Gliomas (Gliomas) are the most common primary malignant tumors of the central nervous system, with invasive growth, easy recurrence, and poor prognosis, making them one of the most challenging diseases in cancer treatment [1]. The annual incidence of glioma is about 6 / 100,000, and the average 5-year survival rate is less than 10%[2]; The mean time to death is less than 18 months [3]. [0003] Currently, surgical resection, chemotherapy combined with radiotherapy and targeted immunotherapy are the main options for the treatment of glioma. However, the average survival time of glioma patients has not been substantially improved due to postoperative residual tumors, decreased radiosensitivity, and chemotherapy resistance. Macrophage migrat...

Claims

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Application Information

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IPC IPC(8): A61K31/505A61P35/00A61P25/00A61P35/04
CPCA61K31/505A61P35/00A61P25/00A61P35/04
Inventor 杜延茹郑琳
Owner 郑琳
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