Extraction method of lactic acid bacteria lysing active matter and lactic acid bacteria lysing active matter obtained by extraction method

An extraction method and a technology of lactic acid bacteria, which are applied in the field of lactic acid bacteria lysate active substances, can solve problems such as the inability to extract lactic acid bacteria cell lysates, and achieve good antioxidant activity, good economic benefits, and high extract content.

Inactive Publication Date: 2022-02-18
GUANGDONG MARUBI BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method uses lysozyme to treat the bacteria and obtain the recombinant lactic acid bacteria by means of the electrode of the high-voltage pulse electroporation instrument, but because the fully fermented lactic acid bacteria are not treated, the purpose of completely extracting the lactic acid bacteria cell lysate cannot be achieved.

Method used

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  • Extraction method of lactic acid bacteria lysing active matter and lactic acid bacteria lysing active matter obtained by extraction method
  • Extraction method of lactic acid bacteria lysing active matter and lactic acid bacteria lysing active matter obtained by extraction method
  • Extraction method of lactic acid bacteria lysing active matter and lactic acid bacteria lysing active matter obtained by extraction method

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Experimental program
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Effect test

Embodiment 1

[0060] This embodiment provides a method for extracting lactic acid bacteria lysing actives, the extraction method of lactic acid bacteria lysing actives comprises the following steps:

[0061] (1) Inoculate lactic acid bacteria in 150 mL of MRS liquid medium, cultivate and activate at 120 r / min, 37° C. for 24 hours to obtain seed liquid; wherein, the inoculum amount of lactic acid bacteria is 3 wt %;

[0062] (2) Inoculate the seed solution obtained in step (1) in the fermentation medium at an inoculum size of 3 wt%, and cultivate it at 150r / min and 37°C for 24h to obtain a fermentation solution;

[0063] Wherein, the fermentation medium includes: peptone 10.0g, yeast extract powder 5.0g, glucose 20.0g, sodium acetate 5.0g, diammonium hydrogen citrate 2.0g, Tween-80 1.0g, K 2 HPO 4 2.0 g, MgSO 4 ·7H 2 O 0.58g, MnSO 4 ·H 2 O0.25g and distilled water 1L;

[0064] (3) The fermented liquid obtained in step (2) is centrifuged at 8000r / min for 10min, washed with water twice,...

Embodiment 2

[0067] This embodiment provides a method for extracting lactic acid bacteria lysing actives, the extraction method of lactic acid bacteria lysing actives comprises the following steps:

[0068] (1) Inoculate lactic acid bacteria in 150 mL of MRS liquid medium, cultivate and activate at 120 r / min, 37° C. for 24 hours to obtain seed liquid; wherein, the inoculum amount of lactic acid bacteria is 3 wt %;

[0069] (2) Inoculate the seed solution obtained in step (1) in the fermentation medium at an inoculum size of 3 wt%, and cultivate it at 150r / min and 37°C for 24h to obtain a fermentation solution;

[0070] Wherein, the fermentation medium includes: peptone 10.0g, yeast extract powder 5.0g, glucose 20.0g, sodium acetate 5.0g, diammonium hydrogen citrate 2.0g, Tween-80 1.0g, K 2 HPO 4 2.0 g, MgSO 4 ·7H 2 O 0.58g, MnSO 4 ·H 2 O0.25g and distilled water 1L;

[0071] (3) The fermented liquid obtained in step (2) is centrifuged at 7000r / min for 10min, washed with water twice,...

Embodiment 3

[0074] This embodiment provides a method for extracting lactic acid bacteria lysing actives, the extraction method of lactic acid bacteria lysing actives comprises the following steps:

[0075] (1) Inoculate lactic acid bacteria in 150 mL of MRS liquid medium, cultivate and activate at 120 r / min, 37° C. for 24 hours to obtain seed liquid; wherein, the inoculum amount of lactic acid bacteria is 3 wt %;

[0076] (2) Inoculate the seed solution obtained in step (1) in the fermentation medium at an inoculum size of 3 wt%, and cultivate it at 150r / min and 37°C for 24h to obtain a fermentation solution;

[0077] Wherein, the fermentation medium includes: peptone 10.0g, yeast extract powder 5.0g, glucose 20.0g, sodium acetate 5.0g, diammonium hydrogen citrate 2.0g, Tween-80 1.0g, K 2 HPO 4 2.0 g, MgSO 4 ·7H 2 O 0.58g, MnSO 4 ·H 2 O0.25g and distilled water 1L;

[0078] (3) The fermented liquid obtained in step (2) is centrifuged at 8000r / min for 10min, washed with water twice,...

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Abstract

The invention provides an extraction method of a lactic acid bacteria lysing active matter and the lactic acid bacteria lysing active matter obtained by the extraction method. The extraction method of the lactic acid bacteria lysing active matter comprises the following steps: diluting lactic acid bacteria sludge to obtain bacteria liquid, and then crushing the bacteria liquid by using a high-voltage pulsed electric field to obtain the lactic acid bacteria lysing active matter. According to the method, the high-voltage pulsed electric field is adopted to crush the lactobacillus sludge obtained by fermentation, cell membrane rupture and intracellular cytoplasm outflow are finally caused by increasing cell membrane permeability and weakening cell membrane strength, and the method has the characteristics of low energy consumption, short treatment time, high efficiency and the like. The high-voltage pulse electric field technology is utilized to denature and inactivate intracellular polysaccharide clastic enzyme, and the polysaccharide decomposition capability is not realized any more, so that the extracted polysaccharide is higher in content and better in antioxidant activity.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation and extraction, and in particular relates to a method for extracting lactic acid bacteria lysing actives and the lactic acid bacteria lysing actives obtained therefrom. Background technique [0002] Acidobacteria are a group of microorganisms that live in the intestinal tract and are beneficial to human health. They can improve the micro-ecological balance of the host, form an antibacterial biological barrier, inhibit the growth and reproduction of pathogenic microorganisms and spoilage bacteria, and play an antibacterial and anti-inflammatory role. The active products of Lactobacillus lysate play an important role in improving the skin. These active metabolites include organic acids, amino acids, vitamins, polysaccharides, small molecular peptides, and biological enzymes. Organic acids can balance the pH of the skin surface, making the pH of the skin weakly acidic, while amino acid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/06
CPCC12N1/066
Inventor 孙怀庆郭朝万张伟强胡露聂艳峰王娟
Owner GUANGDONG MARUBI BIOLOGICAL TECH CO LTD
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