Application of AAV9-CPEB3 in preparation of medicine for treating gastric cancer

1. The technology of AAV9-CPEB3 and AAV9, applied in the field of biomedicine, can solve the problems of cancer cell proliferation, invasion and metastasis of gastric cancer cells, etc., and achieve the effect of improving the quality of life, increasing the chance of cancer recurrence, and easy recurrence

Active Publication Date: 2022-02-25
THE FIRST AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The purpose of the present invention is to provide a kind of pharmaceutical composition AAV9-CPEB3 expressing endogenous CPEB3 protein in the patient's body in the application in the preparation treatment gastric cancer or the postoperative prevention gastric cancer relapse medicine, to solve the existing technology for gastric cancer patient postoperative gastric cancer Cells are easy to invade and metastasize in the human body, leading to technical problems of cancer cell proliferation

Method used

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  • Application of AAV9-CPEB3 in preparation of medicine for treating gastric cancer
  • Application of AAV9-CPEB3 in preparation of medicine for treating gastric cancer
  • Application of AAV9-CPEB3 in preparation of medicine for treating gastric cancer

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Experimental program
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Effect test

Embodiment 1

[0030] Firstly, the gastric cancer cell lines SNU-1, SGC-7901, AGS, MGC-803, HGC-27, NCI-N87, MKN-28, MKN-45 and the human gastric mucosal cell line GES-1 were selected for fluorescence real-time Quantitative PCR detection and Western-blot experiments showed that the expression of CPEB3 messenger RNA and protein was significantly down-regulated in gastric cancer cell lines ( figure 1 A, C).

[0031] Then, 20 fresh gastric cancer tissues and corresponding adjacent non-rumen mucosal tissues were randomly collected from the patients who underwent gastric cancer resection in the First Affiliated Hospital of Sun Yat-Sen University and were confirmed to be gastric adenocarcinoma after surgery, and the tissues were analyzed separately. Fluorescent real-time quantitative PCR detection and Western-blot experiments showed that the expression of CPEB3 messenger RNA and protein was significantly down-regulated in gastric cancer tissues ( figure 1 B,C).

[0032] Subsequently, 15 cases of...

Embodiment 2

[0034] From January to December 2012 in the First Affiliated Hospital of Sun Yat-sen University in the Department of Gastrointestinal Surgery, 160 patients with gastric cancer were randomly selected; from January to December 2013 in the First Affiliated Hospital of Sun Yat-sen University Among the patients undergoing gastric cancer resection in gastrointestinal surgery, 80 patients with gastric cancer were randomly selected. According to certain inclusion and exclusion criteria, 131 patients with gastric adenocarcinoma were finally included in the training cohort, and 68 patients were included in the validation cohort.

[0035] Detailed collection of clinicopathological data, surgical data and postoperative follow-up data of the above patients, such as patient gender, age, comorbidities, serosa invasion, tumor location, operation method, operation time, hemostasis method, intraoperative bleeding and blood transfusion volume , postoperative complications, perioperative death, r...

Embodiment 3

[0076] The following in vitro experiments were carried out on CPEB3 to further confirm its effect on gastric cancer.

[0077] 1. CPEB3 can inhibit the proliferation ability of gastric cancer cells in vitro

[0078] The proliferation ability of gastric cancer cells is often closely related to the growth of gastric cancer. In order to know whether CPEB3 is related to the proliferation ability of gastric cancer, multiple experiments were used to confirm this situation. The growth curve of CCK8 cells is as follows image 3 A shows that the proliferation ability of gastric cancer cells MGC-803 and HGC-27 decreased significantly after overexpression of CPEB3. Such as image 3 B shows that the cell colony formation assay showed that the colony numbers of gastric cancer cells MGC-803 and HGC-27 were significantly reduced after overexpression of CPEB3. These two experiments both confirmed that CPEB3 has a significant relationship with the proliferation ability of gastric cancer, and...

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Abstract

The invention belongs to the technical field of biomedicine, and particularly relates to application of AAV9-CPEB3 in preparation of a medicine for treating gastric cancer. Experiments show that when expression of corresponding protein coded by the CPEB3 gene is reduced in cytoplasm, the postoperative cancer recurrence probability of a patient is increased. Through various known administration modes, expression up-regulation of corresponding protein coded by the CPEB3 gene is realized, so proliferation of gastric cancer cells in a human body after an operation is effectively prevented, the metastasis opportunity of the gastric cancer cells in the human body is reduced, the survival quality of a patient after the operation is thus improved, postoperative rehabilitation is improved, and gastric cancer relapse is prevented.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and specifically relates to the application of AAV9-CPEB3 in the preparation of drugs for treating gastric cancer. Background technique [0002] According to the statistics of global cancer epidemiology in 2020, there are more than 1 million new cases of gastric cancer and about 769,000 deaths, respectively ranking the fifth most common malignant tumor and the fourth leading cause of tumor-related death, posing a serious threat to human life and health. In recent years, with the advancement of diagnostic methods, surgical techniques and comprehensive treatment methods, more choices have been provided for the diagnosis and treatment of gastric cancer, and the curative effect of gastric cancer has been improved to a certain extent. However, gastric cancer is a kind of tumor with highly malignant biological behavior. It is very easy to invade and metastasize in the early stage. Most patients hav...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K48/00A61P35/00A61P35/04
CPCA61K48/005A61K48/0033A61P35/00A61P35/04Y02A50/30
Inventor 何裕隆陈键刘田雨杨东杰李璐傅桦烽
Owner THE FIRST AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
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