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Application of fidaxomicin in preparation of product for resisting mycobacterium fortuitum infection

A technology of Mycobacterium fortuitousus and Fidaxomycin, which is applied in the direction of antibacterial drugs, medical preparations containing active ingredients, organic active ingredients, etc., and can solve problems such as differences in treatment options and different sensitivities

Pending Publication Date: 2022-03-25
TSINGHUA UNIV +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many strains of NTM pathogenic bacteria, different strains of NTM have different sensitivities to drugs, and the treatment options for NTM diseases caused by different strains are also quite different
Moreover, NTM has extremely high drug resistance to anti-tuberculosis drugs, such as isoniazid, rifampicin, streptomycin and other commonly used anti-tuberculosis drugs. Therefore, it is very useful to find drugs with better NTM therapeutic effect. necessary

Method used

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  • Application of fidaxomicin in preparation of product for resisting mycobacterium fortuitum infection
  • Application of fidaxomicin in preparation of product for resisting mycobacterium fortuitum infection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1, Nonopromycin on the Antibacterial Activity Detection of Standard Standard Standard Strains

[0044] Waplers to be tested: non-propamycin

[0045] 1. Add 100 μL Mueller Hinton (MH) medium (including 5% OADC) in each well of 96-well plates;

[0046] 2. After completing the step 1, the 96-well plate is taken to add 100 μl of concentration of 128 μg / ml to the drug solution (formulated by DMSO), and then absorb 100 μL of 100 μL after mixing, and then dilute. After the second column, 100 μL abandoned, the first column did not contain the drug, which was a positive control hole. 3 complement holes are provided for each concentration.

[0047] 3. After completing the step 2, the 96-well plate is taken, and 100 μl of the standard strain suspension of the bonus, which is added to each well, so that the final volume in each well is 200 μl, the final concentration of the bacterial liquid is 2.5 × 10 5 CFU / mL; the final drug concentration in each column is specifically sh...

Embodiment 2

[0059] Example 2, non-propamycin on the detection of antibacterial activity of clinical separation elderranium strains

[0060] Clinical separation strain: 23 strains were isolated from a strain from a sputum specimen in patients with mycobacterial infection, and were identified by 16SRRNA, HSP65, RPOB, and 16-23S rrna zone sequencing.

[0061] Drugs to be tested: non-propamycin.

[0062] In accordance with the method in Example 1, the antibacterial activity of the clinical separation of the 23 strains to be tested to be tested was detected.

[0063] The MIC results are shown in Table 2. MIC concentration distribution statistics are shown in Table 3 and figure 1 Indicated.

[0064] Table 2 Antibacterial activity of non-propamycin on the clinical separation of agonized mycobacterial strains

[0065] Strain number MIC (μg / ml) 1 2 2 4 3 4 4 2 5 32 6 8 7 0.25 8 16 9 4 10 16 11 0.25 12 32 13 4 14 16 1...

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Abstract

The invention discloses an application of fidaxomicin in preparation of a product for resisting mycobacterium fortuitum infection. The invention provides an application of fidaxomicin or a pharmaceutically acceptable salt thereof or a substance taking the fidaxomicin or the pharmaceutically acceptable salt thereof as an active ingredient in preparation of a mycobacterium fortuitum bacteriostatic agent. According to the invention, the anti-mycobacterium fortuitum activity of the fidaxomicin is determined by adopting a microwell plate double dilution method, and the result shows that the fidaxomicin has relatively good bacteriostatic activity on mycobacterium fortuitum standard strains and clinically separated mycobacterium fortuitum; new application of fidaxomicin in prevention and treatment of accidental mycobacterium infection diseases is expected to be explored.

Description

Technical field [0001] The present invention relates to the field of biological technology, and specific to non-vermicomycin is used in the preparation of anti-coupled mycobacterial infection. Background technique [0002] Non-Tuberculous Mycobacteria, NTMs refers to mycobacterium that is divided into complex groups and leprosyl bacillus. NTM is always in a natural environment and is a chance of pathogenic bacteria. In recent years, NTM has gradually rising trend, seriously threatening human health. There was a literature report that the ratio of NTM isolates in my country's cultivated positive specimens increased from 4.3% in 1979 to 22.9% in 2010. NTM pathogenic bacteria has a wide variety, and different strains of NTM have different sensitivity to drugs, and there is a great difference in the treatment regimens for NTM disease caused by different strains. And NTM has extremely high resistance to anti-tuberculosis drugs, such as common anti-tuberculosis drugs such as isoniazid,...

Claims

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Application Information

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IPC IPC(8): A61K31/7048A61P31/04
CPCA61K31/7048A61P31/04
Inventor 李浩黄海荣王桂荣
Owner TSINGHUA UNIV
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