Gene marker for diagnosing spinal cord injury and screening spinal cord injury treatment drugs

A technology for diagnosing spinal cord injury, applied in disease diagnosis, microbial determination/examination, biological testing, etc., can solve the problems of heavy psychological and economic burden of patients and their families, high disability rate of spinal cord injury, and high medical expenses

Pending Publication Date: 2022-03-25
BEIJING CHAOYANG HOSPITAL CAPITAL MEDICAL UNIV
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Spinal cord injury has a high disability rate and high medical expenses, which bring heavy psychological and economic burdens to the patients themselves and their families. So far, there is still no effective treatment

Method used

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  • Gene marker for diagnosing spinal cord injury and screening spinal cord injury treatment drugs
  • Gene marker for diagnosing spinal cord injury and screening spinal cord injury treatment drugs
  • Gene marker for diagnosing spinal cord injury and screening spinal cord injury treatment drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Genome-wide eukaryotic RNA sequencing of traumatic spinal cord injury tissue using next-generation high-throughput sequencing technology.

[0035] 1. Model making

[0036] C57BL / 6 mice were modeled with spinal cord impact injury using the modified Allen's method: the experimental mice were anesthetized by intraperitoneal injection of pentobarbital (75 mg / kg), the T10 spinous process was the central operation area, and the T10 spinous process and lamina were removed to expose the hard spine Membrane, spinal cord injury (10 g, 6.25 mm) was induced using the MASCIS spinal cord injury impactor. After the operation, the wound was sutured layer by layer.

[0037] Signs of successful impact are: spinal cord tissue edema, hemorrhage, complete swelling of the dura mater, purple-red; mice have tail-wagging reflex, lower limbs retract and flutter, and flaccid paralysis.

[0038] In the sham operation group, only the spinal cord was exposed and the wound was sutured layer by laye...

Embodiment 2

[0062] The spinal cord tissues of the experimental animals in the 3 groups were respectively taken and stained with HE. The results are shown in Figure 1 ~ Figure 4 .

[0063] The results showed that in the spinal cord injury group, the structure of the spinal cord was disordered, the boundary between gray and white matter was unclear, the number of neurons was reduced, local hemorrhage, and inflammatory cell infiltration, etc., while the above changes were significantly alleviated in the hyperbaric oxygen treatment group, and the histopathological score was significantly reduced (the higher the score, It indicates that the damage is more serious).

Embodiment 3

[0065] Histopathological evaluation of injured spinal cord

[0066] At different time points after operation, the BMS scores of the hindlimb motor function of the three groups of experimental animals were respectively scored (0 points, completely paralyzed; 9 points, completely normal), suggesting that the BMS scores of the hyperbaric oxygen therapy group at each time point were significantly higher than those of the spinal cord injury group , confirmed that hyperbaric oxygen therapy can significantly promote the recovery of limb function. see results Figure 5 .

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Abstract

Whole-genome eukaryotic RNA sequencing is performed on traumatic spinal cord injury tissues by applying a second-generation high-throughput sequencing technology, so that screening and comparative analysis of gene expression profiles before and after hyperbaric oxygen therapy of traumatic spinal cord injury experimental animals are completed; the gene expression profiles of a hyperbaric oxygen treatment group, a false operation group and an injury group are respectively compared and analyzed, the 9 RNAs have statistical differences in three experimental groups, and the gene can be used for a rapid diagnosis method of spinal cord injury and screening of drugs for spinal cord injury, and is of great significance in diagnosis and treatment of spinal cord injury.

Description

technical field [0001] The invention relates to the technical field of biotechnology, in particular to a gene marker for diagnosing spinal cord injury and screening drugs for treating spinal cord injury. Background technique [0002] Spinal cord injury has a high disability rate and high medical expenses, which bring heavy psychological and economic burdens to patients and their families. So far, there is still a lack of effective treatment methods. With the development of social economy, especially the transportation and construction industry, the incidence of spinal cord injury is increasing year by year. According to statistics, the global incidence of traumatic spinal cord injury is 10.5 cases per 100,000 people, that is to say, there are 768,473 new cases every year in the world, and the number of spinal cord injuries in my country has exceeded one million, and it is increasing at a rate of 120,000 per year. [0003] Spinal cord injuries are divided into primary and se...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883C12N15/11G01N33/68G01N33/573
CPCC12Q1/6883G01N33/6893G01N33/573C12Q2600/158C12Q2600/136G01N2800/28G01N2333/90245G01N2333/47G01N2333/96486
Inventor 刘雪华杨晶苏庆军梁芳孙振程
Owner BEIJING CHAOYANG HOSPITAL CAPITAL MEDICAL UNIV
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