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Urine V-ATPASE subunit S1 and application of polypeptide fragment of urine V-ATPASE subunit S1 in allergic diseases

A technology for allergic diseases and polypeptide fragments, which is used in disease diagnosis, material analysis by electromagnetic means, measurement devices, etc.

Pending Publication Date: 2022-03-29
BEIJING SHIJITAN HOSPITAL CAPITAL MEDICAL UNIVERSTY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, clinical auxiliary examinations for allergic diseases mainly include skin prick test (SPT) and serum sIgE detection, etc. However, patients with SPT test have poor compliance, are easily affected by drugs, and can increase the occurrence of systemic allergic symptoms risk, not suitable for severe allergies and children and elderly groups

Method used

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  • Urine V-ATPASE subunit S1 and application of polypeptide fragment of urine V-ATPASE subunit S1 in allergic diseases
  • Urine V-ATPASE subunit S1 and application of polypeptide fragment of urine V-ATPASE subunit S1 in allergic diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 Urine Specimen Collection and Processing

[0021] Patients with allergic diseases were selected as the allergic disease group, and healthy subjects who underwent physical examination during the same period were selected as the normal control group. Collect 30ml of fresh morning urine samples from the research subjects in each group after admission, and collect the urine in the catheter in the morning for those who cannot urinate normally, and place them in a dry and clean container. After the collected urine samples were centrifuged at 4000r / min for 5min, the supernatant was aspirated, divided into 2ml tubes, and stored in a -80°C refrigerator.

Embodiment 2

[0022] Example 2 Mass Spectrometry and Screening of Urine Peptides

[0023] Extract protein from urine samples and determine the concentration of the extracted protein. Mass spectrometric analysis of urine samples was performed by an OrbitrapFusion type mass spectrometer. Quantitative calculation was performed on the data obtained in the mass spectrometer of the experimental group and the normal control group. The difference between groups was analyzed by t-test, and the difference in protein expression was more than 1.5 times and the statistical test P<0.05 was used as the reference standard to screen for differentially expressed proteins.

Embodiment 3

[0024] Example 3 Identification and Analysis of Differential Peptides

[0025] The database used was the Uniprot_Homo database, and the original mass spectrum files were processed by MaxQuant software. The search parameter settings are shown in Table 1.

[0026] Table 1 Max Quant software retrieval parameter list

[0027]

[0028] Compared with healthy people, V-ATPASE subunit S1 was less expressed in the urine of patients with allergic diseases, and its content in the urine of healthy controls and allergic disease groups was as follows: figure 1 As shown, there is a significant difference in the expression of V-ATPASE subunit S1 in the urine of the normal control group and the allergic disease group.

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Abstract

The invention provides an application of a urine V-ATPASE subunit S1 (V-type proton ATPase subunit S1) and a polypeptide fragment of the urine V-ATPASE subunit S1, and particularly relates to an application of the urine V-ATPASE subunit S1 and the polypeptide fragment of the urine V-ATPASE subunit S1 in preparation of preparations for allergic disease diagnosis, differential diagnosis, illness degree judgment, treatment effect evaluation, monitoring, prognosis evaluation, mechanism research and the like. Researches prove that compared with a healthy person (a normal control group), the expression of the urine V-ATPASE subunit S1 and the polypeptide fragment thereof in an allergic disease patient is reduced. The method can be used for various purposes of application detection of allergic disease patients. According to the invention, the advantages of noninvasive acquisition, large-scale repeated sampling and convenient preservation of a urine sample are exerted, and the urine sample is used for detecting the urine V-ATPASE subunit S1 and the polypeptide fragment thereof.

Description

technical field [0001] The present invention relates to new uses of urine V-ATPASE subunit S1 and its polypeptide fragments, in particular to the use of urine V-ATPASE subunit S1 and its polypeptide fragments in the diagnosis, differential diagnosis, judgment of disease severity, evaluation of treatment effects, The application of preparations such as monitoring, prognosis evaluation and mechanism research. Background technique [0002] Allergic diseases are also known as allergic diseases. Typical allergic diseases include allergic rhinitis, bronchial asthma, allergic dermatitis, eczema, allergic conjunctivitis and anaphylactic shock. According to the statistics of the World Health Organization, the number of allergic people in the world is huge, and about one-third of the people have suffered from allergic diseases. In recent years, due to the rapid changes in people's lifestyles, the significant increase in the degree of industrialization, and the obvious aggravation of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/573G01N27/62
CPCG01N33/573G01N27/62G01N2333/914G01N2800/24G01N2800/52
Inventor 张曼刘娜
Owner BEIJING SHIJITAN HOSPITAL CAPITAL MEDICAL UNIVERSTY
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