Nanometer delivery system for regulating and controlling functions of microenvironment T cells as well as preparation method and application of nanometer delivery system

A delivery system and microenvironment technology, applied in the fields of biomedical engineering and chemistry, can solve the problem of lack of non-specific drug absorption, and achieve the effect of promoting proportional change and immune activity, reducing tissue distribution and release, and reducing toxic and side effects

Active Publication Date: 2022-04-12
THE FIRST AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0015] In summary, there is currently a lack of nanocarrier systems that can effectively avoid non-specific drug absorption by the mother and fetus, and then achieve specific drug delivery and functional regulation of helper T cells in the placenta

Method used

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  • Nanometer delivery system for regulating and controlling functions of microenvironment T cells as well as preparation method and application of nanometer delivery system
  • Nanometer delivery system for regulating and controlling functions of microenvironment T cells as well as preparation method and application of nanometer delivery system
  • Nanometer delivery system for regulating and controlling functions of microenvironment T cells as well as preparation method and application of nanometer delivery system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Synthesis of S1, polyacetylimide grafted polyethylene glycol (PEG-PEI)

[0065] Polyethyleneimine-grafted polyethylene glycol (PEG-PEI) was synthesized by a two-step method. First, the terminal hydroxyl groups of monomethyl ether polyglycol were activated with carbonyldiimidazole, and then reacted with the amino groups of polyethyleneimine to generate PEG. -PEI. The specific operation is as follows: weigh monomethyl ether glycol (8.0 g, Mn=2 kDa) into a reaction flask, dry it in vacuum at 80° C. for 6 h, and add THF (60 mL) under an argon atmosphere to dissolve it. Weigh carbonyldiimidazole (CDI, 6.4g) and another reaction bottle, then slowly drop THF dissolved in mPEG-OH into the CDI bottle with a constant pressure dropping funnel, and stir the reaction overnight at room temperature. Distilled water (0.648 mL) was added to inactivate excess CDI, and stirring was continued for 30 min. Precipitate the solution into a large amount of cold ether, filter and dry in vacuo ...

Embodiment 2-4， comparative example 1-6

[0086] Compared with Example 1, Example 2-4 or Comparative Example 1-6 can be prepared by changing the dosage of polymer, drug and SPIO in Step S3 or omitting a certain step in Steps S3, S4, S5, S6, and S7 , see Table 1 below for details:

[0087] Table 1: Examples and comparative examples

[0088]

[0089]

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Abstract

The invention discloses a nano delivery system for microenvironment T cell function regulation and a preparation method and application thereof.The delivery system is of a core-shell double-layer structure, and an enzyme substrate polypeptide-PEG modified lipid bimolecular membrane which is disintegrated in a targeted mode under the action of high-expression enzyme in contact with placenta tissue interstitial fluid serves as a shell; a drug carrier modified by an important immunomodulatory cell helper T cell (Th) surface specific high-expression marker antibody in a placenta is used as an inner core, and superparamagnetic ferroferric oxide SPIO nanoparticles, a small molecule drug for regulating and controlling the function of the helper T cell in the placenta, a therapeutic gene or a combination thereof are loaded in the drug carrier. The nano delivery system for regulating and controlling the functions of the T cells in the microenvironment can effectively avoid non-specific drug absorption of other organs outside the placenta of the maternal body and a fetus, so that specific drug delivery and function regulation and control of helper T cells in the placenta are realized.

Description

technical field [0001] The invention relates to the fields of chemistry and biomedical engineering, in particular to a nano-delivery system for microenvironment T cell function regulation and its preparation method and application. Background technique [0002] Threatened miscarriage is a common disease during pregnancy. It refers to irregular vaginal bleeding before the 28th week of pregnancy, but there is no abnormal pregnancy substance discharge. If this situation is not effectively controlled, it will eventually lead to fetal miscarriage and even affect the health of the mother. According to previous statistics, the disease accounted for 15% of the number of pregnancies. However, with the introduction of the second- and third-child policy, the proportion of elderly pregnant women has increased, and the incidence and severity of threatened abortion have shown a clear upward trend year by year, seriously threatening the health of pregnant women and fetuses, and even affec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/127A61K47/42A61K47/34A61K47/68A61K31/37A61K31/713A61K48/00A61K41/00A61P15/06A61P37/02
Inventor 郭宇
Owner THE FIRST AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
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