Method for identifying main components for treating mastitis in coronet aqueous extract

Abstract

The invention discloses a method for identifying main components for treating mastitis in a coronet aqueous extract, and belongs to the technical field of traditional Chinese medicine identification. The method comprises the following steps: screening component peaks with a remarkable effect on treating mastitis in a coronet aqueous extract, identifying a single-component peak with an optimal effect on treating mastitis, and carrying out liquid chromatography-mass spectrometry combined analysis on the single-component peak with the optimal effect from the protein level, so as to obtain a single-component peak with an optimal effect on treating mastitis. According to the score, the protein abundance, the polypeptide modification site and the secondary mass spectrum, identifying the main component for treating mastitis in the single-component peak with the optimal effect; by screening and identifying active ingredients with anti-inflammatory action in a single-component peak of the antler plate, a powerful experimental reference is provided for deep development and clinical application of the antler plate; the screening and verification problems of antler plates and even animal traditional Chinese medicine complex components are solved, and the method has great significance in narrowing the range of traditional Chinese medicine effective components and screening and determining the traditional Chinese medicine effective components.

Description

technical field [0001] The invention relates to the technical field of identification of traditional Chinese medicines, in particular to an identification method for the main components in the water extract of antler disc for treating mastitis. Background technique [0002] As a traditional animal Chinese medicine, antler plate has a medicinal history of more than 2,000 years. It not only has the effects of warming kidney yang, strengthening bones and muscles, promoting blood circulation and reducing swelling, but also can treat breast addiction, mastitis, mumps and other diseases. . In nature, the antler disc can be attached to the horn handle of living tissue for up to half a year, but it has a miraculous biological phenomenon that it can fall off naturally without inflammation, suppuration, or swelling, which shows that the antler disc has good anti-inflammatory properties effect. Throughout modern medicine and scholars' scientific research on antler discs, for mastitis...

AI Technical Summary

Problems solved by technology

Throughout modern medicine and scholars' scientific research on antler discs, for mastitis, so far, no pharmacological efficacy experiments and anti-inflammatory mechanism research have been conducted in strict scientific experiments, and no effective medicinal extracts have been extracted and isolated. Element

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