Preparation, feed addition and application method of feed additive for promoting growth of small yellow croakers

A technology of feed additive and small yellow croaker, which is applied in the forming or processing of animal feed, application, animal feed, etc. It can solve the problems of high temperature resistance of active protein molecules, degradation and inactivation of intestinal protease, and can not be added, etc. It is easy to reach the production scale Scale-up, high biological activity, and low production cost

Pending Publication Date: 2022-04-15
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to aim at the growth-promoting active protein molecules of small yellow croaker produced in bioreactors, which are poorly absorbed in the intestinal tract of fish after oral administration, are easily degraded and inactivated by intestinal proteases, and the active protein molecules are not resistant to high temperature and cannot be processed before feed granulation. Addition, easy to dissolve in water after spraying and other problems, the purpose of this invention is to provide a growth-promoting feed additive for small yellow croaker

Method used

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  • Preparation, feed addition and application method of feed additive for promoting growth of small yellow croakers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] A small yellow-gum-proof growth feed additive preparation and adding method, this method is carried out in the following steps:

[0057] Preparation of silkworm pupa: 100g of the bioreactor of -20 ° C freezing, 2-3 hours in the temperature of 4 ° C, was tapered in the tissue homogenizer, the highest speed homogenate 30 seconds, stop 1 minute, repeated 8-10 times.

[0058] Adjurators: It is weighed 0.2 g C10 (sodium citrate), dissolved in 20 ml of water, and prepared into a 1% C10 solution.

[0059] Mixing of pucemus and adjuvant: Pour the C10 solution into the pucemium, mix stir evenly.

[0060] Mixed with binder: 4 g of acrylamide adhesive powder, poured into the mixture of adjuvants and the mixture of the tie, and gently stir it until slurry.

[0061] Mixed with merchants: 2kg merchandise pellets in the face machine, stir the stirring, and then poured into the slurry bioactive additive, stirred for 10-15 minutes.

[0062] Drying and preservation of feed: prepared for feed...

Embodiment 2

[0064] Example 1 In this step (2): 0.2 g of C10 (sodium citrate), dissolved in 20 ml of water, is prepared into a 1% C10 solution; in step (4): 4G polysaccharide binder powder; The remaining steps are in Example 1.

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Abstract

The invention relates to the technical field of aquatic feed bio-additives, in particular to a feed additive for promoting growth of pseudosciaena crocea prepared by adopting gene edited bioreactor silkworm chrysalis as well as a preparation method, feed and application of the feed additive. The feed additive for promoting growth of the small yellow croakers comprises the following components in parts by mass: 100 parts of bioreactor silkworm chrysalis, the bioreactor silkworm chrysalis expresses fusion protein of growth hormone and transferrin of the small yellow croakers, and the feed additive further comprises the following components: 0.1-0.5 part of sodium citrate; and 2.0 to 10.0 parts of an adhesive. According to the invention, the proper adhesive is screened, so that the adhesion of active protein and commodity granules is improved, and the dissolution loss of active protein molecules in water is reduced; a proper oral adjuvant is screened, and the working concentration is determined, so that the intestinal permeability of active protein is improved, degradation is reduced, the bioavailability of the additive is improved, and the growth promotion effect of the additive is realized.

Description

Technical field [0001] The present invention relates to the technical field of aquatic feed biological additive, and more particularly to a gene edited bioreactor silkworm cocoon prepared a small yellow-fish growth feed additive and feed addition and application. Background technique [0002] Small yellow fish, is one of my country's four seafood economic fishs, which is widely distributed in the East China Sea, Huanghai and Bohai and the West Bank of the North Korean Peninsula. Its meat is delicate and delicate, the nutritional value is high, and the consumer is loved. However, due to excessive fishing and marine environment variation, small yellow fish resources have been severely damaged, although the resources have resumed in the implementation of volt season, but is still very fragile. In recent years, with the maturity of small yellow culprit, the number of artificial farming has increased year by year, providing a better solution for resource protection and demand contradi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K50/80A23K10/20A23K20/105A23K20/163A23K40/10
Inventor 翁宏飚王彦华沈卫锋楼宝
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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