Preparation method of celery seed extract
A celery seed extract and a celery seed technology are applied in the field of preparation of the celery seed extract to achieve the effects of low cost, good economic benefits, and inhibition of free radical scavenging
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Embodiment 1
[0024] Example 1 Preparation and Condition Optimization of Celery Seed Extract
[0025] 1. Screening of ultrasonic extraction conditions
[0026] (1) Weigh 1 kg of washed and dried celery seeds, add 25-40L of 20-50% ethanol solution, and extract with 40kHz ultrasonic wave at 25-55°C for 15-90 min to obtain ethanol extract;
[0027] (2) Add the ethanol extract to the molecular distillation equipment, set the scraper speed to 35 rpm, the scraper temperature to 85°C, the vacuum degree to 2mto, and the feed rate to be 1.0 mL / min to collect the liquid in the light component bottle to obtain the extract ;
[0028] (3) Freeze-dry the extract at -50°C for 48 hours to obtain celery seed extract powder.
[0029] Calculate the extraction amount (g / 100g) according to the quality of the raw material celery seed and the extract to perform univariate screening on the extraction conditions, the results are as follows figure 1 shown. It can be seen from the results that as the concentratio...
Embodiment 2
[0035] Example 2 Inhibition of uric acid production by celery seed extract
[0036] 1. Extract Preparation
[0037] (1) Weigh 1 kg of washed and dried celery seeds, add 35L of 35% ethanol solution, and extract with 40 kHz ultrasonic wave at 45°C for 1 hour to obtain ethanol extract;
[0038] (2) Put the ethanol extract into the molecular distillation equipment, set the scraper speed to 35 rpm, the scraper temperature to 80°C, the vacuum degree to 2 mto, and the feed rate to be 1.0 mL / min to collect the liquid in the light fraction bottle to obtain celery Rapeseed Extract ( image 3 A);
[0039] (3) Freeze-dry the celery seed concentrate at -50°C for 48 hours to obtain the following: image 3 The celery seed extract powder shown in B is designated as sample 1.
[0040] According to the method of Example 2 of CN110585252A, the flavonoid-rich celery seed extract was obtained, which was recorded as sample 2.
[0041] According to the method of CN112716989A Example 1, the cele...
Embodiment 3
[0052] Example 3 The scavenging effect of celery seed extract on free radicals
[0053] 1. Extract Preparation
[0054] (1) Weigh 1 kg of washed and dried celery seeds, add 35L of 35% ethanol solution, and extract with 40 kHz ultrasonic wave at 45°C for 1 hour to obtain ethanol extract;
[0055] (2) Put the ethanol extract into the molecular distillation equipment, set the scraper speed to 35 rpm, the scraper temperature to 80°C, the vacuum degree to 2 mto, and the feed rate to be 1.0 mL / min to collect the liquid in the light fraction bottle to obtain celery rapeseed extract;
[0056] (3) The celery seed concentrate was freeze-dried at -50°C for 48 hours to obtain celery seed extract.
[0057] Referring to the method of Liu Changjiang et al. (Liu Changjiang, Liu Hui, Li Bin, Li Yitong. A comparative study on the extraction of celery seed essential oil by different methods [J]. Food Science and Technology, 2009, v.34; No.210(04): 182-185) using water Extraction of celery seed ...
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