Preparation for preventing and treating bacterial diseases and application thereof

A bacterial and preparation technology, applied in the field of preparations for the prevention and control of bacterial diseases, can solve the problems of reduced control effect, evolution of copper-resistant bacteria, environmental pollution, etc., and achieves the effects of no drug resistance, high selectivity, and easy degradation.

Active Publication Date: 2022-04-26
XINJIANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, sodium hypochlorite, 46% copper hydroxide, 72% agricultural streptomycin sulfate, etc. are used to prevent and control this disease. Long-term use of these pesticides leads to resistance of plants and reduces the control effect. The amount of pesticide application continues to increase, bringing agricultural products. Residues of pesticides, severe environmental pollution, and a certain concentration of copper ions will cause watermelon acidovorous bacteria to enter a viable but non-culturable state (viable but nonculturable state, VBNC), and there are subgroup I strains in the BFB pathogen population. Copper ions are not sensitive, and copper-containing chemical pesticides or fungicides can temporarily effectively prevent BFB, but long-term use will cause watermelon acidovorax bacteria to be insensitive to copper ions, and there is a huge risk of evolving into copper-resistant bacteria

Method used

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  • Preparation for preventing and treating bacterial diseases and application thereof
  • Preparation for preventing and treating bacterial diseases and application thereof
  • Preparation for preventing and treating bacterial diseases and application thereof

Examples

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preparation example Construction

[0027] In the present invention, the preparation method of the Allium pilosula inflorescence extract comprises the following steps: mixing and extracting the Allium pilosula inflorescence and an extractant to obtain the Allium pilosula inflorescence extract. In the present invention, when said mixing, the mass volume ratio of the Allium scape and the extractant is preferably 1.5g:10mL; that is, the solid-liquid ratio is 1.5:10; in the present invention, the inflorescence of the Allium scape and the extraction The mass-volume ratio of the agent can be expanded in equal proportions, that is, the mass-volume ratio of the inflorescence of Scape edulis and the extractant can be 3g: 20mL, or 4.5g: 30mL, and so on. In the present invention, the inflorescences of the Allium pilosula are preferably the inflorescences at the full flowering stage of the Allium pilosula, more preferably the inflorescences at the full flowering stage of the Allium pilosula, specifically as figure 1 Shown; ...

Embodiment 1

[0039] The preparation of the material (water extract of Scape inflorescence) for preventing and treating bacterial diseases:

[0040] The ratio of material to liquid is 1.5:10 (m:V) to get the inflorescence (15g, referred to as the inflorescence of Allium pilosula) and sterile water (100mL) during the full flowering stage of the green onion dried to constant weight. Mix the inflorescences of Scape scallion with sterile water, place it in a dark and cool place at room temperature for extraction for 48 hours, and stir once every 12 hours; centrifuge the stirred material at 3000r / min for 10min, collect the supernatant, and obtain the control Bacterial disease preparation (mass concentration of 150mg / mL Scape inflorescence sterile water extract);

[0041] The preparations for preventing and treating bacterial diseases were diluted with sterile water into preparations with final mass concentrations of 50 mg / mL, 100 mg / mL and 150 mg / mL respectively, which were recorded as preparati...

Embodiment 2

[0043] The preparation of the material (methanol extract of the inflorescence of Scape scallion) for the prevention and treatment of bacterial diseases:

[0044] According to the ratio of material to liquid of 1.5:10 (m:V), take the inflorescence powder (15g, referred to as the inflorescence powder of Allium scaping) and methanol (100mL) during the full flowering stage of Allium scaping dried to constant weight, wherein the inflorescence powder of Allium scaping The particle size is between 0.01 and 0.45mm. Mix the inflorescences of Scape scallion dried to constant weight with methanol, place it in a dark and cool place at room temperature for 72 hours, stir once every 12 hours, and collect the supernatant After suction filtration with 0.22 μm filter paper, use a rotary evaporator to concentrate (60-70°C, 45min) to obtain a preparation for preventing and treating bacterial diseases (the mass concentration is 150mg / mL methanol extract of the onion inflorescence, that is, 1ml ext...

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Abstract

The invention belongs to the technical field of disease control, and particularly relates to a preparation for controlling bacterial diseases and application thereof. The invention provides a preparation for preventing and treating bacterial diseases. The effective component of the preparation comprises a scallion lepidii inflorescence extract. The preparation for preventing and treating the bacterial diseases is the extract containing the scallion inflorescence, has an inhibiting effect on proliferation and growth of pathogenic bacteria of the bacterial diseases, especially acidovorax citrulli, and meanwhile has the advantages that the pathogenic bacteria are not prone to generating resistance, and pollution is avoided.

Description

technical field [0001] The invention belongs to the technical field of disease prevention and control, and in particular relates to a preparation for preventing and treating bacterial diseases and its application. Background technique [0002] Watermelon Acidovorax is the pathogen of bacterial fruit blotch (BFB), which infects Cucurbitaceae plants in a high-temperature and high-humidity environment, and occurs on melons, cucumbers, watermelons and other melons, causing plant disease. spots, wilting or fruit rot. In the context of world economic integration, the disease has spread in many countries through global trade activities, such as the United States, Australia, Brazil, Japan, Mariana Islands, Indonesia, Turkey and other countries, especially in melon producing areas. diseases that are difficult to control. At present, sodium hypochlorite, 46% copper hydroxide, 72% agricultural streptomycin sulfate, etc. are used to prevent and control this disease. Long-term use of t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N65/42A01N59/20A01P1/00
CPCA01N65/42A01N59/20Y02A50/30
Inventor 林辰壹李文静王宁
Owner XINJIANG AGRI UNIV
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