The preparation method of the radiant crosslink porous reticular acellular amnion hydro gel dressing

Abstract

The invention discloses a preparation method of an irradiation crosslinked porous network decellularized amniotic membrane hydrogel dressing. The specific preparation method is: a hydrogel solution composed of polyvinyl alcohol, chitosan and acrylic acid-acrylamide copolymer , put it into the stainless steel plate that holds the net-like porous decellularized amniotic membrane, add a small amount of agar, shake it well, let it stand still and evacuate it to get the hydrogel prototype; then press it into a hydrogel membrane; use 60Coy of the membrane - Radiation cross-linking at room temperature, then dehydration, freeze-drying, radiation sterilization, and packaging under sterile conditions. The present invention has good protective and therapeutic effects, such as pain relief, anti-inflammatory and antibacterial, healing promotion, etc., and has good water absorption, water retention, air permeability and flexibility, and can also be used according to sensitive bacteria on the wound surface, pain conditions, According to the needs of hemostasis or blood circulation, the corresponding drugs can be selectively selected and the drugs can be released slowly to achieve the purpose of assisting treatment.

Description

Technical field: [0001] The invention relates to the field of medical biological dressings, in particular to a preparation method of an irradiation cross-linked porous network decellularized amniotic membrane hydrogel dressing capable of absorbing and slowly releasing newly prepared drugs. Background technique: [0002] Modern burn treatment not only focuses on improving the survival rate of patients, but also emphasizes the recovery of function. Finding an ideal skin substitute is very important for the early coverage and later restoration of large burn wounds. An ideal skin substitute should meet the following conditions: (1) low cost; (2) long shelf life: (3) can be applied after resurrection; (4) non-antigenic; (5) persistent; (6) Flexibility; (7) It can prevent water loss; (8) It has a barrier effect on bacteria; (9) It has good compliance to irregular wounds; (10) It is easy to fix; (11) It can grow and expand accordingly with the growth of children ; (12) The transpl...

AI Technical Summary

Problems solved by technology

[0003] The most research in the prior art is the preparation and preservation of amniotic membrane. As the patent publication number is CN1522568A and the patent publication number is CN1201697A, although the biological amniotic membrane described in CN1201697A can be stored at room temperature, it is used clinically because it is not decellularized. is limited; the Patent Publication No. CN1757717A overcomes the above-mentioned deficiencies in the preparation, and adopts the decellularization technology to make a single-layer decellularized dry amniotic membrane, which is more practical and preserved than the previous two, but it is still a single-layer amniotic membrane. These amniotic membranes A common shortcoming is: thin, poor mechanical properties, resistance, easy to tear, easy to curl, difficult to plant and shape, easy to dissolve after covering the wound, etc.

So its clinical effect is not as good as the dressing combined with bioactive amniotic membrane

Moreover, the way of its slow-release medicine is first soaked in the pre-selected and fixed medicine solution to swell and then pressed into a finished product, which has the disadvantage of not being able to treat symptoms according to the specific situation of the wound. At the same time, if it is not used in time, the medicine effect will be weakened.