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Bacillus WYJ-E14 separated from curcuma wenyujin and application of bacillus WYJ-E14 in preparation of antitumor drugs

A technology of WYJ-E14, Bacillus, applied in antitumor drugs, medical preparations containing active ingredients, drug combinations, etc., to achieve the effects of rich metabolites, strong anti-stress ability, and high application value

Active Publication Date: 2022-04-26
HANGZHOU NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few reports about the anti-tumor effect of plant endophytes at home and abroad.

Method used

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  • Bacillus WYJ-E14 separated from curcuma wenyujin and application of bacillus WYJ-E14 in preparation of antitumor drugs
  • Bacillus WYJ-E14 separated from curcuma wenyujin and application of bacillus WYJ-E14 in preparation of antitumor drugs
  • Bacillus WYJ-E14 separated from curcuma wenyujin and application of bacillus WYJ-E14 in preparation of antitumor drugs

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1: Isolation and cultivation of Bacillus sp. WYJ-E14.

[0020] 1. Materials and methods

[0021] 1. Experimental materials:

[0022] The cultivar of Wenyujin: Wenyujin No. 1, donated by Zhejiang Wenzhou Tianhe Biotechnology Co., Ltd. Wen Yujin is planted in a pot in a glass house of the School of Life and Environmental Sciences, Hangzhou Normal University.

[0023] 2. The medium used in the experiment:

[0024] The composition of the PDA liquid medium is as follows: 200 g of peeled potatoes are cut into small pieces, boiled with 1000 mL of water, filtered with two layers of gauze to obtain the filtrate, added with 20 g / L of glucose, 15 g / L of agar, and pH 7.0.

[0025] The composition of MS basic medium is: NH 4 NO 3 1.65 g / L, KNO 3 1.9 g / L, CaCl 2 2H 2 O 0.44g / L, MgSO 4 ·7H 2 O 0.37g / L, KH2PO4 0.17 g / L, KI 0.83mg / L, H3BO35.2 mg / L, MgSO4 7H2O 22.3mg / L, ZnSO4 7H2O 3.6mg / L, Na2MoO4 2H2O 0.25mg / L, CuSO4·5H2O 0.025mg / L, CoCl2·6H2O 0.025mg / L, Na2EDTA37.3 m...

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Abstract

The invention discloses bacillus WYJ-E14 separated from curcuma wenyujin and application of the bacillus WYJ-E14 in preparation of antitumor drugs. The endophyte separated from the rhizome part of curcuma wenyujin belongs to bacillus velezensis. The bacillus velezensis is widely distributed in nature, has good characteristics of rapid growth and genetic stability, is harmless to human and animals, and does not pollute the environment. The bacillus subtilis is rich in metabolite, has broad-spectrum antibacterial activity and relatively strong anti-stress capability, and plays a more and more important role in many fields such as agriculture, environment, fermentation industry and the like. In the invention, the bacillus velezensis WYJ-E14 has an extremely remarkable anti-tumor cell effect, and has a very high application value in the aspect of development of biological anti-tumor drugs.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a plant endophyte and its application in the preparation of anti-tumor drugs, in particular to a bacillus WYJ-E14 isolated from curcuma curcuma and its application in the preparation of anti-tumor drugs. Background technique [0002] Curcuma wenyujin (Curcuma wenyujin Y.H.Chen et C.Ling) is a plant of the genus Curcuma longa in the Zingiberaceae family. It is mainly distributed in Wenzhou, Zhejiang Province. It can not only be used as an ornamental flower, but also the whole plant can be used for medicinal purposes. The medicinal material "Wenyujin" processed from its tubers is one of the famous authentic medicinal materials "Zhejiang Eight Flavors" in Zhejiang Province. [0003] According to reports, nearly 100 kinds of chemical components have been isolated from turmeric, the main types are monoterpenoids, sesquiterpenoids, diterpenoids, alkaloids and so on. Modern pharmacological s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/742A61P35/00A23L33/135C12R1/07
CPCA61K35/742A61P35/00A23L33/135A23V2002/00A23V2200/308Y02A50/30
Inventor 向太和黄小平曾章慧陈哲皓王利琳庞基良童霞秀李亚菲
Owner HANGZHOU NORMAL UNIVERSITY