Stem cell blood-free culture device and automatic culture method thereof

Abstract

The invention discloses a stem cell blood-free culture device and an automatic culture method thereof. The stem cell blood-free culture device comprises a heat preservation box, a dust cover is installed at the top of the heat preservation box, supporting plates are installed at the four top corners of the bottom of the heat preservation box, and the culture box is installed in the center of the top of the interior of the heat preservation box. The device has the beneficial effects that the device is provided with a rotating assembly, the position of the culture dish can be adjusted through the rotating assembly, a connecting block can move left and right through telescopic movement of a pneumatic cylinder, a rotating cylinder, a rotating plate and a limiting block on a rotating rod are used in cooperation, and synchronous rotation of a connecting pipe and a connecting plate can be achieved; according to the device, the mounting assembly is arranged, and in the process of placing the culture dishes, the culture dishes only need to be pressed into the placing grooves of the mounting plate, so that the culture dishes can be quickly fixed.

Description

technical field [0001] The invention relates to a culture device, in particular to a stem cell bloodless culture device and an automatic culture method thereof, belonging to the technical field of stem cell bloodless culture. Background technique [0002] Serum-free media and reagents are widely used to culture mammalian and invertebrate cells to prepare monoclonal antibodies, viral antigens and recombinant proteins, etc. Most serum-free media contain transferrin and Insulin that regulates glucose uptake, etc. These proteins perform various functions in cell culture, such as providing the matrix required for cell attachment, resisting shear forces in bioreactors, and serving as carriers of lipids and other growth and differentiation factors, etc. . [0003] Since the serum-free medium lacks the natural components in serum to neutralize toxins and protect cells and macromolecules, most of the existing medium needs to manually add nutrient solution to it, and the staff is in ...

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Problems solved by technology

[0003] Since the serum-free medium lacks natural components in serum to neutralize toxins and protect cells and macromolecules, most of the existing medium needs to manually add nutrient solution to it, and the staff is in the process of adding nutrient solution to the inside of the medium In the medium, the medium is susceptible to death due to the influence of the external environment, and it needs to be moved during the cultivation of serum-free stem cells, and it is easy to shake during the moving process, which is likely to cause the death of stem cells inside the incubator, increasing the probability of stem cell death

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