Use of nmn for preventing and/or treating pain and corresponding composition
One-NMN, the technology of use, applied in the direction of drug combination, non-central analgesics, medical preparations containing active ingredients, etc., can solve problems such as obstructed respiratory muscles, adverse reactions, and allergies to conventional analgesics
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Embodiment 1
[0407] The effectiveness of using NMNs according to the invention was evaluated in a rat nociceptive pain model. More precisely, the model used is the visceral pain model. Administration of cyclophosphamide (CYP) was used as a method to simulate cystitis in rats.
[0408] The positive control was ibuprofen, a non-steroidal anti-inflammatory drug commonly used to relieve nociceptive pain. Negative controls were NMN and ibuprofen vehicle, ie, distilled water.
[0409] In this study, 7-week-old female Sprague-Dawley rats were divided into three groups, each consisting of 6 rats:
[0410] - control group receiving 5 ml of distilled water (vehicle);
[0411] - the group treated with 500 mg / kg of NMN; and
[0412] - Group treated with 300 mg / kg ibuprofen.
[0413] NMN is in the zwitterionic form.
[0414] After 24 hours of acclimatization, the pain tolerance threshold of each animal was measured using von Frey fibrils before the start of the study. Measurements were taken pri...
Embodiment 2
[0436] Synthesis of compounds of the present invention
[0437] Materials and methods
[0438] All reagents were obtained from commercial suppliers and used without further purification. Thin layer chromatography was performed on TLC silica gel 60F254 plastic plates (0.2 mm layer thickness) from Merck. Column chromatography purification was performed on silica gel 60 (70-230 mesh ASTM, Merck). Melting points were determined either on a digital device (electrothermal IA 8103), uncorrected, or on a Kofler heating stage WME type (Wagner & Munz). 1 H. 19 F and 13 C Nuclear magnetic resonance (NMR) and infrared (IR) spectroscopy confirmed the structures of all compounds. Infrared spectra were recorded on a Perkin Elmer Spectrum 100FT-IR spectrometer; and NMR spectra were recorded using CDCl 3 、CD 3 CN, D 2 O or DMSO-d 6 as solvent, recorded at 300 or 400 MHz on a BRUKER AC 300 or 400 spectrometer 1 H spectrum, recorded at 75 or 100MHz 13 C Spectrum, recorded at 282 or 37...
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