Plant extract composition as well as preparation method and application thereof

A technology of plant extracts and compositions, which can be used in pharmaceutical formulations, cosmetic preparations, toiletry preparations, etc., and can solve problems such as expensive prices, product discoloration, and high cost pressures

Pending Publication Date: 2022-05-03
广州萃普生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These ingredients are also likely to cause quality problems in cosmetics: the application of arbutin in the formula can easily cause product discoloration; kojic acid can chelate with many metal ions, especially iron complex ions, and slowly oxidize when in contact with air and color. ...

Method used

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  • Plant extract composition as well as preparation method and application thereof
  • Plant extract composition as well as preparation method and application thereof
  • Plant extract composition as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1: the preparation of Moringa oleifera seed extract

[0025] (1) Preparation of Moringa Seed Water Extract:

[0026] Dry the Moringa oleifera seeds at 80°C, crush them, pass through a 50-mesh sieve, add deionized water according to the liquid-to-material ratio of Moringa oleifera seed powder to deionized water 1:12, and perform ultrasonic extraction at 30°C for 40 minutes, with an ultrasonic power of 1000W. The working intermittent ratio is 2s / 2s, the circulating pump speed is 25r / s, after standing still, take the supernatant, filter it with gauze, let the filtrate stand still, and then centrifuge the supernatant, the centrifugal speed is 3000-4000r / min, and the centrifugation time is 10min , take the supernatant and carry out vacuum rotary evaporation, the vacuum degree of vacuum rotary evaporation is 0.07-0.09MPa, to obtain the Moringa oleifera seed aqueous extract.

[0027] (2) Preparation of Moringa oleifera oil extract:

[0028] Dry the leaves of Morin...

Embodiment 2

[0030] Embodiment 2: test sample is to the inhibitory rate of tyrosinase activity

[0031] The inhibitory effect of samples on tyrosinase activity was determined by dopa rate method.

[0032] As shown in Table 1, add L-tyrosine, sample solution, and PBS buffer solution to the tubes numbered A1, A2, B1, and B2 in sequence, and mix well: put the test tube rack into a 37°C constant temperature water bath Water bath for 10-15 minutes: add tyrosinase to each tube of group A1 and B1 in turn, shake well, time accurately, and stop the reaction for 15 minutes: spot the plate on a 96-well plate in turn, measure its absorbance with a microplate reader and calculate the inhibition Rate. The formula for calculating the inhibition rate is:

[0033]

[0034] In the formula, A 1 Absorbance of the enzyme system for the blank sample; A 2 Absorbance of blank sample without enzyme system; B 1 is the absorbance of the enzyme system in the sample group; B 2 Absorbance of the sample group w...

Embodiment 3

[0040] Example 3: Kinetic analysis of the inhibitory effect of Moringa oleifera seed extract on tyrosinase.

[0041] The mode of action of inhibitors and enzymes can be divided into reversible inhibition and irreversible inhibition, among which reversible inhibition can be divided into competitive type, non-competitive type, anti-competitive type and linear mixed type, which can be determined by Lineweaver-Burk double reciprocal graph judge.

[0042] The concentration of fixed tyrosinase is constant, and the inhibitory effect of the sample on tyrosinase activity is measured by the dopa rate method, see Example 2, and the concentration of L-tyrosine measured respectively is 0.5, 1, 1.5, 2mmol / L When the mass concentration is 0.1, 0.25, 0.5, 1mg / mL of the absorbance of the Moringa seed extract, draw the concentration-ΔA curve, see figure 2 , figure 2 The relationship between the mass concentration of Moringa oleifera seed extract and the inhibition rate of tyrosinase.

[00...

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Abstract

The invention discloses a plant extract composition and application thereof, the plant extract composition comprises a moringa seed extract and glabridin, the moringa seed extract is 0.25-3 mg/mL, and the glabridin is 0.25-5 mg/mL; a preparation method of the moringa seed extract comprises the following steps: (1) adding deionized water into a moringa seed raw material, carrying out ultrasonic extraction, taking supernate, filtering, standing, centrifuging, and carrying out rotary evaporation to dryness, so as to obtain a moringa seed water extract; (2) adding 30-70% ethanol into the moringa seed raw material, performing ultrasonic extraction, taking supernate, filtering, standing, centrifuging, and performing rotary evaporation to dryness to obtain a moringa seed oil extract; and (3) mixing the hot pepper aqueous extract and the moringa seed oil extract to obtain the moringa seed extract. When the concentration of the compound agent is 0.25 mg/mL, the inhibition effect of the compound agent on tyrosinase is far better than that of the glabridin and the moringa oleifera extract which are independently used, and the inhibition effect of the compound agent on tyrosinase is better than that of common arbutin and nicotinamide.

Description

technical field [0001] The invention relates to the technical field of cosmetics, in particular to a plant extract composition and its application. Background technique [0002] It is the development direction of today's whitening products to achieve whitening effect by inhibiting the synthesis of melanin. At present, arbutin, kojic acid, vitamin C and their derivatives have been widely used in whitening products and achieved certain effects. However, the penetration rate of the above-mentioned whitening active ingredients is low, and the efficacy is not ideal enough. Formulators often enhance the effect by adding a large amount of whitening agents, but it is still difficult to meet the needs of consumers. Simultaneously, along with adding amount increases, produced many potential safety hazards. For example, studies have shown that long-term application of kojic acid can cause contact dermatitis and erythema reactions, and may induce breast cancer in women; arbutin achiev...

Claims

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Application Information

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IPC IPC(8): A61K8/9789A61K8/49A61Q19/00A61Q19/02
CPCA61K8/9789A61K8/498A61Q19/00A61Q19/02A61K2800/782A61K2800/82
Inventor 涂禄清邓建科蔡永通
Owner 广州萃普生物科技有限公司
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