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Application of IFI35 in preparation of medicine for preventing or treating colorectal cancer

1. IFI35, colorectal cancer technology, applied in the direction of drug combination, pharmaceutical formula, anti-tumor drugs, etc., can solve problems such as unknown function

Pending Publication Date: 2022-05-03
THE SIXTH AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have shown that IFI35 plays an important role in innate immunity and antiviral immunity, but its function in tumor immunity is unknown

Method used

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  • Application of IFI35 in preparation of medicine for preventing or treating colorectal cancer
  • Application of IFI35 in preparation of medicine for preventing or treating colorectal cancer
  • Application of IFI35 in preparation of medicine for preventing or treating colorectal cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 To clarify the correlation between IFI35 and CD8+ T cell infiltration in colorectal cancer and its clinical significance

[0033] (1) The expression of CD8 in 128 patients with colorectal cancer was detected by immunohistochemical method, and the proteomic sequencing data of 128 patients with colorectal cancer were analyzed by combining with bioinformatics, which proved that IFI35 in tumor was positively correlated with the expression of CD8+ T cells.

[0034] The expression of CD8+ T cells in 128 patients with colorectal cancer was detected by SP two-step method, and the expression level was scored. Combined with bioinformatics analysis of the protein profile data of these 128 patients, genes related to CD8 expression were found, and it was found that IFI35 was positively correlated with CD8+ T cell expression.

[0035] (2) The correlation analysis between the expression of IFI35 and the prognosis of patients.

[0036] The survival data of patients in the ho...

Embodiment 2

[0037] Example 2 Elucidating that IFI35 affects the infiltration of CD8+ T cells in the tumor immune microenvironment of colorectal cancer and its anti-tumor immune function

[0038] (1) Construction of IFI35 knockdown and overexpression colon cancer cell lines.

[0039] ①Using molecular cloning technology, the pSin vector was used to construct a colorectal cancer cell line overexpressing IFI35, and the pLKO.1 vector was used to construct a colorectal cancer cell line with knockdown of IFI35.

[0040] ②The lentivirus was packaged in 293T cells, and then the colorectal cancer cell lines were infected with the lentivirus, and the knockdown and overexpression efficiencies were detected by WB and qPCR.

[0041] (2) In vitro CCK8 proliferation experiments showed that IFI35 had no effect on the proliferation of colorectal cancer cells.

[0042] Colorectal cancer cells were plated on a 96-well plate, and the culture plate was incubated in an incubator for 24H, 48H, and 72H; CCK8 was a...

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Abstract

The invention relates to the field of immunotherapy, in particular to application of IFI35 in preparation of a medicine for preventing or treating colorectal cancer. A colorectal cancer animal model shows that IFI35 can directly regulate CD8 + T cell infiltration and enhance immune response; the method comprises the following steps: analyzing the change of CD8 < + > T cells and T cell functional cytokines (IFN-gamma, Granzyme B and Perforin) in a tumor microenvironment by using a flow cytometry and an immunohistochemical method; the animal model verifies the curative effect of the IFI35 recombinant protein combined with other immunotherapy means. The completion of the invention clarifies that IFI35 is a key molecule affecting the infiltration and function of CD8 + T cells in tumors, and provides a new idea and a potential target for immunotherapy.

Description

technical field [0001] The invention relates to the field of immunotherapy, in particular to the application of IFI35 in the preparation of a medicament for preventing or treating colorectal cancer. Background technique [0002] Colorectal cancer is one of the malignant tumors with the highest morbidity and mortality worldwide, and its incidence ranks third and second in men and women, respectively. The incidence of colorectal cancer in my country is increasing year by year. In 2015, there were 376,000 new cases, which is one of the five tumors with the highest incidence in my country. In recent years, with the continuous improvement of surgical skills, the application of preoperative concurrent chemoradiotherapy and targeted drugs, the survival time of patients with advanced colorectal cancer has been improved, but the five-year survival rate of patients with advanced colorectal cancer is still less than 50%. The survival rate of stage IV patients is even lower than 10%, s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K45/00A61P35/00
CPCA61K45/00A61P35/00
Inventor 叶树标兰平李佩思陈东雯龙家慧
Owner THE SIXTH AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
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