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Microscopic imaging device and method based on wavefront correction of spatial light modulator

A spatial light modulator and wavefront correction technology, applied in the field of optical imaging, can solve the problems of fewer microscopic objective lenses, test acquisition of aberration information, and increase the difficulty of microscopic objective aberrations, etc., to achieve simple steps, stable structure, and imaging The effect of quality improvement

Pending Publication Date: 2022-05-10
SHANGHAI INST OF OPTICS & FINE MECHANICS CHINESE ACAD OF SCI
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Problems solved by technology

However, there is very little information available for the microscopic objective lens, neither the original design file nor the aberration information can be obtained through a simple test, which greatly increases the difficulty of correcting the aberration of the microscopic objective lens

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  • Microscopic imaging device and method based on wavefront correction of spatial light modulator
  • Microscopic imaging device and method based on wavefront correction of spatial light modulator
  • Microscopic imaging device and method based on wavefront correction of spatial light modulator

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[0026] In order to make the purpose, technical solution and advantages of the present invention easier to understand, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, and are not intended to limit the present invention, and the equivalent replacement of a certain element or multiple elements in the embodiments described in the present invention is still within the protection scope of the present invention Inside.

[0027] An ideal objective lens is a point when it is focused at a specified object-space refractive index. When there is a cover glass 9 near the focal point, due to the difference in the refractive index of the cover glass and the refractive index during focusing, the light beam that was originally focused on one point will become a focal line distributed along the optical ...

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Abstract

The invention relates to a microscopic imaging device and method based on wavefront correction of a spatial light modulator, which can be used for realizing microscopic object lens aberration correction imaging caused by cover glass of any specification and correcting aberration of a microscopic object lens and other systems at the same time. The device utilizes a wavefront sensor to detect wavefront behind an objective lens and utilizes a spatial light modulator to compensate. According to the device, compensation test pieces for manufacturing cover glass with different thicknesses are omitted, and the device also has a good correction effect on aberration of a microscope objective and other systems, and has a great application value in the field of optical microscopic imaging.

Description

technical field [0001] The invention relates to the field of optical imaging, specifically a wavefront correction imaging device based on wavefront sensor detection, which can be used to correct the imaging of microscopic objective aberrations caused by any specification of cover glass, and at the same time correct the imaging of objective aberrations and other systems The difference is corrected. Background technique [0002] The microscope can be divided into two groups of objective lens and eyepiece. The objective lens and eyepiece of a traditional microscope are relatively closed through the lens barrel connection structure and cannot continue to perform aberration compensation, and only need to achieve simple observation, so the requirements for imaging are not high. However, with the continuous deepening of scientific research needs, the objective lens and eyepiece are relatively separated, which provides the possibility for subsequent aberration compensation. However...

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Application Information

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IPC IPC(8): G02B21/36G02B21/32G02B21/00G06T7/00
CPCG02B21/365G02B21/32G02B21/00G06T7/0002G06T2207/30168
Inventor 曾爱军乔适胡敬佩董延更黄惠杰
Owner SHANGHAI INST OF OPTICS & FINE MECHANICS CHINESE ACAD OF SCI
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