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Primer probe combination and kit for vagina micro-ecology detection

A technology for detecting primers and detection probes, applied in the direction of microorganisms, methods based on microorganisms, measurement/inspection of microorganisms, etc., to achieve the effects of reducing recurrence frequency, improving amplification specificity, and improving detection specificity

Active Publication Date: 2022-05-13
BEIJING ORIGIN-POLY BIO-TEC CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no corresponding detection product on the market to meet clinical needs

Method used

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  • Primer probe combination and kit for vagina micro-ecology detection
  • Primer probe combination and kit for vagina micro-ecology detection
  • Primer probe combination and kit for vagina micro-ecology detection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 Determination of cutoff value of vaginal microbial composition

[0023] For Lactobacillus crispatus (LC), Lactobacillus gasseri (LG), Lactobacillus jennis (LJ), Lactobacillus inert (LI), Gardnerella vaginalis (GV), Candida (CA) and vaginal hair droplet The known gene sequences of worms (TV) were compared and analyzed to obtain their respective differential gene sequences, the detection primers and probes were designed for the differential gene sequences, and the detection primers and probes of the internal reference gene GAPDH were also added.

[0024] The specific nucleotide sequences of the designed primers are shown in the table below:

[0025] Table 1. Detection primer sequences corresponding to each microbial target gene

[0026]

[0027] Note: F represents the forward detection primer, R represents the reverse detection primer, and G represents the internal reference gene GAPDH.

[0028]Add a 3-5bp sequence to the 5' end of the above primer sequence...

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PUM

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Abstract

The invention discloses a primer probe combination and a kit for vaginal micro-ecology detection, the primer probe combination comprises target gene detection primers and probes for lactobacillus crispatus (LC), lactobacillus gasseri (LG), lactobacillus jensenii (LJ), lactobacillus inertus (LI), gardnerella vaginalis (GV), candida (CA) and trichomonas vaginalis (TV), and the probes are used for melting curve typing. The kit comprises a primer probe combination. Through combination of the specific primers and the probes, specific target genes of different microorganisms can be effectively amplified, melting curve analysis can be performed on amplification products, the seven microorganisms can be detected in one tube, and the method has the advantages of high specificity, short time consumption, high sensitivity, comprehensive detection site coverage and the like; auxiliary diagnosis reference can be provided for clinicians, treatment can be carried out in advance, and clinical application is wide.

Description

technical field [0001] The invention relates to the technical field of gene detection, in particular to a primer set and a kit for risk detection of vaginal micro-ecological balance. Background technique [0002] The human female vaginal environment refers to the micro-ecological environment composed of all the microbial groups living in the female vaginal cavity. Under normal circumstances, a large number of microorganisms reside in the female vaginal cavity, and these microorganisms play an important role in the local microenvironment to maintain the health of the female vagina. Studies have shown that there are significant differences in the vaginal microbes of women of different ages. It is shown that before puberty, the typical characteristics of female vaginal flora are low abundance of Lactobacillus, Gardnerella vaginalis and R. With the onset of puberty, the vaginal epithelium thickens again, stimulated by estrogen, which favors colonization of the vagina by glucose...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/689C12Q1/6895C12Q1/6893C12Q1/686C12N15/11C12R1/225C12R1/01C12R1/725C12R1/90
CPCC12Q1/689C12Q1/6895C12Q1/6893C12Q1/686C12Q2600/16C12Q2563/107C12Q2545/114C12Q2527/107
Inventor 王林海刘沛
Owner BEIJING ORIGIN-POLY BIO-TEC CO LTD
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