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Non-alcoholic fatty liver animal model and establishment method

An animal model, non-alcoholic technology, applied in the field of animal husbandry, etc., can solve the problems of unobvious blood lipid and fatty liver characteristics, single animal model characteristics of rats, long animal model establishment period, etc., to achieve low cost and improve establishment period , the effect of high similarity

Pending Publication Date: 2022-05-17
北京基谱生物科技有限公司
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  • Claims
  • Application Information

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Problems solved by technology

In traditional animal models, rats are mostly used. However, the characteristics of rat animal models are single, only the characteristics of obesity, the characteristics of blood lipid and fatty liver are not obvious, or only the characteristics of fatty liver, and the characteristics of dyslipidemia are poor
Such an animal model has low clinicopathological similarity with NAFLD and has limited reference value
[0004] In addition, the animal models in the prior art also have disadvantages such as long establishment period and poor stability.

Method used

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  • Non-alcoholic fatty liver animal model and establishment method
  • Non-alcoholic fatty liver animal model and establishment method
  • Non-alcoholic fatty liver animal model and establishment method

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Embodiment 1

[0052] In this example, NR4A1- / - knockout mice and C57BL / 6N mice were used to raise them separately, to illustrate that the method of the present invention can establish a non-alcoholic fatty liver animal model, and at the same time verify that the animal model of the present invention is accompanied by Features such as obesity and dyslipidemia.

[0053] The animal feeds in the comparative experiment were ordinary feed and high-fat feed (D12492) purchased from Parker Bio; NR4A1- / - knockout mice were purchased from Jackson Laboratory in the United States, and C57BL / 6N mice were purchased from Beijing Weitong Lihua Experimental Animals Technology Co., Ltd.

[0054] Wherein, the specific components of the high-fat feed (D12492) and the mass percentages of each component are 20% protein, 20% carbohydrate, and 60% fat; the high-fat feed has higher fat content than ordinary feed.

[0055] In this comparative experiment, SPF grade male NR4A1- / - gene knockout mice and C57BL / 6N mice w...

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Abstract

The invention relates to the technical field of animal model establishment, in particular to a non-alcoholic fatty liver animal model and an establishment method. The animal model is established by adopting an NR4A1- / -gene knockout mouse. The NR4A1- / -gene knockout mouse is adopted to establish the non-alcoholic fatty liver animal model, the relationship between the NR4A1- / -gene and glycometabolism and fat metabolism of the liver is fully utilized, the glycolipid metabolism of the NR4A1- / -gene knockout mouse is disturbed, and fat can be deposited on the liver after high-fat feeding, so that the non-alcoholic fatty liver is formed; the method is simple to operate and obvious in effect. The animal model has the characteristics of obesity, dyslipidemia and the like, and has high similarity with NAFLD clinical pathology.

Description

technical field [0001] The invention relates to the technical field of animal model establishment, in particular to an animal model of non-alcoholic fatty liver and its establishment method. Background technique [0002] Non-alcoholic fatty liver disease (NAFLD) is a clinicopathological syndrome characterized by excessive fat deposition in liver cells, excluding alcohol and other definite liver-damaging factors. It can be divided into primary and secondary There are two types of episodic, the former is related to insulin resistance and genetic susceptibility, while the latter is caused by some special reasons. Nonalcoholic fatty liver disease (NAFLD) is often accompanied by symptoms such as obesity, insulin resistance, hypertension, and dyslipidemia. In recent years, with the development of the economy, the improvement of people's living standards and the changes in the diet structure, the incidence of fatty liver has been increasing year by year. Therefore, it is of great...

Claims

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Application Information

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IPC IPC(8): A01K67/027
CPCA01K67/0276A01K2207/25A01K2227/105A01K2267/03A01K2267/0362
Inventor 来丽丽池喜峰王宇赵成成
Owner 北京基谱生物科技有限公司
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