Unlock instant, AI-driven research and patent intelligence for your innovation.
Micro-fluidic chip
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A microfluidic chip and pipeline technology, applied in the field of biomedical testing, can solve problems such as product blanks, reduce the amount of reagents, and improve the detection range.
Pending Publication Date: 2022-05-17
FUDAN UNIV +1
View PDF0 Cites 0 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
[0004] At present, the detection of CAR-T cells and cytokines in China is mainly through flow cytometry and traditional ELIS
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Example Embodiment
[0035] Example 1
[0036] The present embodiment provides a microfluidic chip (length× width× height of 10cm×8cm×1cm), for simultaneous detection of CAR-T cells and a variety of cytokines in the patient's peripheral blood, including: from top to bottom sequentially connected valve layer, pipe layer and basal layer;
[0038] The sample mixing module 9 comprises: a sample mixing pipe in an "S" shape; the sample mixing pipe is provided with a first inlet for injecting dilution solution 1, a second inlet for injecting fluorescent antibody solution 2 and a third inlet for injecting a venous blood sample 3, the sample mixing pipeline is provided with a first outlet port 4;
[0051] The present embodiment provides a method for simultaneously detecting CAR-T cells and a variety of cytokines in the peripheral blood of patients, the steps comprising:
[0052] One drop (50 μL) of peripheral blood sample, 50 μL of fluorescent antibody solution, and 50 μL of PBS dilution solution were injected at a flow rate of 5 μL / s from the third inlet 3, the second inlet 2 and the first inlet of the sample mixing module 9 as described in Example 1, respectively; in the sample mixing pipeline, the peripheral blood sample was diluted, and the CAR-T cells therein efficiently bound to the fluorescent antibody in the fluorescent antibody solution;
[0053]3-fold diluted peripheral blood sample in CAR-T cell capture detection module 6, T cells and CAR-T cells are captured by T cell capture antibodies, and then fluorescent CAR-T cells and T cells can be detected at the same time, and the proportion of CAR-T cells can be calculated;
[0054] Most of the T c...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
PUM
Property
Measurement
Unit
Height
aaaaa
aaaaa
Length
aaaaa
aaaaa
Login to View More
Abstract
The invention relates to a micro-fluidic chip, which is used for simultaneously detecting CAR-T cells and various cytokines in peripheral blood of a patient, and comprises a valve layer, a pipeline layer and a substrate layer which are sequentially connected from top to bottom, the pipeline layer comprises a sample mixing module, a CAR-T cell capturing and detecting module, a concentration gradient generating module, a cell filtering module and a cell factor detecting module which are sequentially connected through pipelines; the micro-fluidic chip can be used for simultaneously detecting CAR-T cells and cell factors in peripheral blood of a cancer patient receiving CAR-T cell therapy, a whole blood sample does not need to be transferred into a flow cytometer for detection, samples with different concentrations can be simultaneously detected in the micro-fluidic chip, the detection range is effectively expanded, and the detection cost is reduced. A reaction pipeline of the micro-fluidic chip is a closed environment, so that a relatively stable detection environment is provided for detection of CAR-T cells and cell factors; and the blood sample size required by detection is 50 microliters and is less than that required by the existing detection technology, and the dosage of corresponding reagents is also reduced.
Description
Technical field [0001] The present invention relates to the field of biomedical detection technology, in particular to a microfluidic chip. Background [0002] Chimeric Antigen Receptor T-Cell Immunotherapy (Chimeric Antigen Receptor T-CellImmunootherapy), or CAR-T therapy, is a new type of precision-targeted therapy for the treatment of tumors, which is reshaping the treatment of patients with advanced cancer, especially those with limited treatment options, and is effective in the treatment of relapsed, refractory B-cell non-Hodgkin lymphoma, and may be extended to other solid and liquid tumors. A fast, accurate new tumor immunotherapy, which is considered to have the potential to cure cancer, has brought new hope to cancer treatment. However, CAR-T therapy is also accompanied by a series of life-threatening adverse reactions such as cytokine release storms (CRS) and neurotoxicity (NE). The study found that the aggravation of NE is related to the high car-T cell replication le...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to View More 
