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Recombinant adeno-associated virus expression vector and application thereof

A viral vector and expression vector technology, applied in the direction of virus/phage, virus, application, etc., can solve the problems of unsatisfactory chemotherapy effect of glioblastoma, low degree of promoter methylation, etc., achieving less side effects and specificity strong effect

Pending Publication Date: 2022-05-17
THE FIRST AFFILIATED HOSPITAL OF ARMY MEDICAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the use of temozolomide (TMZ) in the treatment of glioblastoma has problems such as the blood-brain barrier (BBB), the low degree of methylation of the O6-methylguanine-DNA methyltransferase (MGMT) gene promoter, etc.
Apparently, glioblastoma chemotherapy with temozolomide as an essential drug is not effective

Method used

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  • Recombinant adeno-associated virus expression vector and application thereof
  • Recombinant adeno-associated virus expression vector and application thereof
  • Recombinant adeno-associated virus expression vector and application thereof

Examples

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Embodiment Construction

[0040] The examples given are for better description of the content of the present invention, but the content of the present invention is not limited to the examples given. Therefore, non-essential improvements and adjustments to the implementation by those skilled in the art based on the content of the invention above still fall within the protection scope of the present invention.

[0041] (1) Construction of hGFAP-promoter-dsacas9-VP64-3flag-polyA vector, specifically:

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Abstract

The invention belongs to the technical field of biology, and particularly relates to a recombinant adeno-associated virus expression vector and application thereof. An expression vector of the recombinant adeno-associated virus comprises an hGFAP-promoter-dsacas9-VP64-3flag-polyA virus vector, a U6-saGRNA (HUWE1) * 3 virus vector, a U6-sagRNA (NC) virus vector, a pAAV-RC vector and a pH (Potential of Hydrogen) elper plasmid, and the expression vector of the recombinant adeno-associated virus comprises a recombinant adeno-associated virus expression vector, a recombinant adeno-associated virus expression vector, a recombinant adeno-associated virus expression vector and a recombinant adeno-associated virus expression vector. The recombinant adeno-associated virus expression vector disclosed by the invention can realize endogenous overexpression of HUWE1 in glioblastoma by utilizing SadCas9, is strong in specificity and small in side effect, and provides a good theoretical and practical basis for treatment of glioblastoma.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a recombinant adeno-associated virus expression vector and its application. Background technique [0002] Tumors that grow in the brain are commonly referred to as brain tumors, including primary brain tumors that originate from the brain parenchyma and secondary brain tumors that metastasize to the brain from other parts of the body. The etiology of brain tumors is unknown. , pituitary gland, cranial nerves, cerebrovascular and embryonic remnants, known as primary intracranial tumors. Secondary intracranial tumors are those that metastasize to the intracranial from malignant tumors in other organs and tissues of the body. [0003] According to biological characteristics, primary brain tumors can be divided into benign brain tumors and malignant brain tumors. Among them, glioma is the most common malignant brain tumor of the central nervous system. According to the Worl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/864C12N15/113C12N15/55C12N15/66C12N15/90A61K38/17A61K48/00A61P35/00
CPCC12N15/86C12N15/113C12N9/22C12N15/66C12N15/907A61K38/1703A61K48/005A61K48/0008A61P35/00C12N2750/14143C12N2310/20Y02A50/30
Inventor 袁野汪黎鸿王姣韦森罗韬卞修武
Owner THE FIRST AFFILIATED HOSPITAL OF ARMY MEDICAL UNIV