Nucleic acid composition for detecting FGF19 gene overexpression, kit and application thereof

Abstract

The invention relates to a nucleic acid composition for detecting FGF19 gene overexpression, a kit and application thereof. Specifically, the nucleic acid composition comprises an upstream primer of the FGF19 gene, a downstream primer of the FGF19 gene and a probe of the FGF19 gene, and the upstream primer of the FGF19 gene is selected from at least one of a nucleic acid sequence shown as SEQ ID NO: 1, a nucleic acid sequence shown as SEQ ID NO: 7, a nucleic acid sequence shown as SEQ ID NO: 8 and / or a nucleic acid sequence shown as SEQ ID NO: 9. A downstream primer of the FGF19 gene has a nucleotide sequence as shown in SEQ ID NO: 2, a nucleotide sequence as shown in SEQ ID NO: 10, a nucleotide sequence as shown in SEQ ID NO: 11 and / or a nucleotide sequence as shown in SEQ ID NO: 12, and a probe of the FGF19 gene has a nucleotide sequence as shown in SEQ ID NO: 3. By adopting the nucleic acid composition, the overexpression multiple of the FGF19 gene can be directly and quantitatively detected, and the rapid detection of the gene overexpression can be completed only by using a common fluorescent quantitative PCR instrument.

Description

technical field [0001] The invention relates to the technical field of molecular diagnosis, in particular to a nucleic acid composition, kit and application for detecting the overexpression of FGF19 gene. Background technique [0002] At present, the method commonly used to detect the overexpression of FGF19 gene is the sandwich method enzyme-linked immunosorbent assay (ELISA), which uses the principle of color development by combining antibodies and enzyme complexes, and finally evaluates the degree of FGF19 in the sample by judging the depth of the color. Express the situation. The detection of FGF19 gene overexpression by sandwich enzyme-linked immunosorbent assay (ELISA) has the following disadvantages: (1) The operation is complicated, time-consuming and laborious, and the antibody cost is high; (2) The accuracy is relatively low, and it is easy to misdetect and miss detection, etc. , poor accuracy and specificity, and low sensitivity; (3) easily affected by cross-cont...

AI Technical Summary

Problems solved by technology

The detection of FGF19 gene overexpression by sandwich enzyme-linked immunosorbent assay (ELISA) has the following disadvantages: (1) The operation is complicated, time-consuming and laborious, and the antibody cost is high; (2) The accuracy is relatively low, and it is easy to misdetect and miss detection, etc. , poor accuracy and specificity, low sensitivity; (3) easily affected by cross-contamination; (4) unable to give quantitative results

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