Key enzyme gene MaSUS2.2 for improving amylopectin content of banana fruits and application of key enzyme gene MaSUS2.2

Abstract

The invention provides a key enzyme gene MaSUS2.2 for improving the amylopectin content of banana fruits, the nucleotide sequence of the key enzyme gene MaSUS2.2 is shown as SEQ ID NO: 1, and the invention further provides protein coded by the gene MaSUS2.2 and application. The MaSUS2.2 gene can regulate the expression of the banana MaSUS2.2 gene, regulate the total starch content of fruits and regulate the amylopectin content in the fruits, and basically does not influence the amylose content, for example, when the MaSUS2.2 gene is introduced into the banana fruits, the gene MaSUS2.2 is over-expressed, and the amylopectin content in the fruits is not influenced. The total starch content of the banana fruits and the amylopectin content in the banana fruits are increased and the amylose content is basically not influenced, or the gene MaSUS2.2 is silently expressed, so that the total starch content of the banana fruits and the amylopectin content in the banana fruits are reduced and the amylose content is basically not influenced; a candidate gene resource is provided for improving the amylopectin quality of bananas or other fruits.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a key enzyme gene MaSUS2.2 for increasing the amylopectin content of banana fruit and its application. Background technique [0002] Starch is the main storage form of photosynthetic products and carbohydrates in higher plants, and is also the main source of carbohydrates, calories and energy in human diet (Chen et al., 1984; Vandeputte et al., 2004; Munyikwa et al., 1997); It is widely distributed in food crops such as rice, wheat, corn, and potato, as well as starch-transformed fruits such as banana, kiwi, and mango (Vetrano et al., 2009; Xu Yong, 2011; Miao Hongxia et al., 2013). Starch is divided into amylose and amylopectin according to the different polymerization forms of the basic unit α-D-glucose (Rochat et al., 1992), of which amylopectin accounts for about 70-80% of the total starch. Therefore, the level of amylopectin directly affects the yield, quality and p...

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Problems solved by technology

do Nascimento et al. (2000) reported that during the starch accumulation process of banana fruit, the SUS activity was strong; during the postharvest ripening process, the SUS activity decreased to a very low level; it was speculated that there may be a correlation between SUS activity and starch metabolism, but experimental evidence has been lacking It proves whether the SUS gene directly acts on the starch synthesis of banana fruit. It is not clear which type of starch the SUS gene acts on in the plant, whether it is amylose synthesis or amylopectin synthesis. No relevant reports have been seen so far.

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