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Polypeptide and cryopreservation solution containing polypeptide

A cryopreservation solution, cryopreservation technology, applied in the preservation of human or animal body, peptide source, animal/human peptide, etc., to achieve the effect of protecting cell damage, stabilizing cell membrane, and excellent biocompatibility

Inactive Publication Date: 2022-05-27
君创永晟(东莞)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no commercial cryopreservation solution and application that uses the conserved domain of the LEA protein as a cryoprotectant to freeze various types of cells

Method used

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  • Polypeptide and cryopreservation solution containing polypeptide
  • Polypeptide and cryopreservation solution containing polypeptide
  • Polypeptide and cryopreservation solution containing polypeptide

Examples

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preparation example Construction

[0075] The preparation method of the present invention will be described in further detail below with reference to specific examples. It should be understood that the following examples are only for illustrating and explaining the present invention, and should not be construed as limiting the protection scope of the present invention. All technologies implemented based on the above content of the present invention are covered within the intended protection scope of the present invention.

[0076] The experimental methods used in the following examples are conventional methods unless otherwise specified; the reagents, materials, etc. used in the following examples can be obtained from commercial sources unless otherwise specified.

Embodiment 1

[0077] Example 1LEA protein functional domain polypeptide

[0078] Table 2 LEA protein functional area

[0079] label amino acid sequence name Molecular weight (D) LS 1-1 RAEQLGHEGYQEMGQKGGQ LEA1-F 2103.27 LS 2-1 EKKGIMDKIKEKLPG LEA2-K 1714.11 LS 2-2 SSSSSEDD LEA2-S 812.7 LS 2-3 RTDEYGNPVH LEA2-Y 1187.24

[0080] The present invention conducts further research through solid-phase synthesis, taking the LEA protein functional region in Table 2 as an example.

Embodiment 2

[0081] Example 2 LEA protein functional region polypeptide frozen mesenchymal stem cells

[0082] 1.Material:

[0083] Mesenchymal stem cells: purchased from Beijing Botaisi Biotechnology Co., Ltd.;

[0084] Commercial vitrification solution containing 10% DMSO: purchased from Beijing Baicaotech Biotechnology Co., Ltd.

[0085] 2. Method

[0086] (1) Preparation of cryopreservation solution and comparative example

[0087] Cryopreservation solution 1 (lea1-F):

[0088] Prepare the cryopreservation solution according to the following formula, and each 100mL contains the following components:

[0089] substance content Lea1-F(g) 1.0 Ethylene glycol (mL) 10 Sucrose (mol L -1 )

[0090] Cryopreservation solution 2 (lea2-K):

[0091] Prepare the cryopreservation solution according to the following formula, and each 100mL contains the following components:

[0092] substance content Lea2-K(g) 1.0 Ethylene glycol (mL) 10 ...

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PUM

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Abstract

The invention discloses a polypeptide and a cryopreservation solution containing the polypeptide, and the polypeptide is a functional region of LEA protein, has good biocompatibility in an aqueous solution, and is an ideal ice control material. The cryopreservation liquid containing the polypeptide is used for cryopreservation of various cells, has a high survival rate, can only achieve the effectiveness of freezing various cells by a conventional freezing liquid, and has a cryopreservation recovery rate equivalent to that of a commercialized cryopreservation liquid containing 10% of DMSO which is commonly used at present.

Description

technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to a polypeptide and a cryopreservation solution containing the polypeptide and the application of these polypeptides and the cryopreservation solution containing the polypeptide. Background technique [0002] Cryopreservation refers to the preservation of biological materials under ultra-low temperature, which slows down or stops cell metabolism and division, and can continue to develop once the normal physiological temperature is restored. The development of modern medicine has experienced chemical medicine, biological medicine, and has moved towards cellular medicine. However, cells cultured in vitro (ex vivo) undergo replicative senescence and death. Therefore, "cells" become ready-to-use drugs on the shelf, and the first problem to be solved is storage. As the only technology currently expected to realize long-term storage and long-distance transportation of "cells", c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/47C12N15/12A01N1/02
CPCC07K14/47A01N1/0221
Inventor 李娜
Owner 君创永晟(东莞)生物科技有限公司
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